Virulence genes of Shiga toxin-producing Escherichia coli O157:H7 strains isolated from calves and cattle

;ŞEKER, KUYUCUOĞLU Yahya

doi:10.1501/vetfak_0000002484

Ankara Üniversitesi Veteriner Fakültesi Dergisi

2011

DOI: 10.1501/vetfak_0000002484

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Virulence genes of Shiga toxin-producing Escherichia coli O157:H7 strains isolated from calves and cattle

KUYUCUOĞLU Yahya ;ŞEKER¹

Abstract: Summary:The aim of this study was to detect E. coli O157:H7 serotype in the faeces samples collected from calves and cattle farms located of Afyonkarahisar province in Turkey and to determine the stx(1) (Shiga toxin 1) and stx(2) (Shiga toxin 2) virulence genes in the strains of E.coli O157:H7 by multiplex PCR (mPCR). In this study, E. coli O157:H7 was isolated from the 3.1% (14 out 457 faeces samples) of the calves and cattle examined, in particular 2.3% of the cattle, 2.6% of non-diarrhoeic calves and 10.6% … Show more

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RETRACTED ARTICLE: Molecular detection of pathogenic Escherichia coli strains and their antibiogram associated with risk factors from diarrheic calves in Jimma Ethiopia

Ali

Tesema

Belachew

2021

Sci Rep

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Diarrheagenic Escherichia coli are a number of pathogenic E. coli strains that cause diarrheal infection both in animal and human hosts due to their virulence factors. A cross sectional study was conducted between November, 2016 and April, 2017 to isolate and molecularly detect pathogenic E. coli from diarrheic calves to determine the pathogenic strains, antibiogram and associated risk factors in Jimma town. Purposive sampling technique was used to collect 112 fecal samples from diarrheic calves. Conventional culture and biochemical methods were conducted to isolate E. coli isolates. Molecular method was followed to identify virulence factors of pathogenic E. coli strains. Antimicrobial sensitivity patterns of the isolates were tested using the Kirby–Bauer disk diffusion method. A structured questionnaire was also used to collect information from dairy farms and socio-demographic data. The overall isolation rate of E. coli in calves was 51.8% (58/112) (95% CI 42.0–61.0). The occurrence of the bacterium differed significantly by age, colostrum feeding time, amount of milk given per time and navel treatment (P < 0.05). Multivariable analysis revealed that the odds of being infected was significantly highest in calves which fed 1–1.5 L amount of milk per a time (OR 5.38, 95% CI 1.66–17.45, P = 0.005). The overall virulence genes detection rate was 53.5% (95% CI 40.0–67.0). Eleven (19.6%) of eaeA, 6 (10.7%) of Stx1 and 13 (23.2%) of Stx2 genes were detected from calves isolates. Except ciprofloxacillin, all isolates were resistant to at least one drug. Multi drug resistance was recorded in 68.0% (38/56) of calves isolates. Neomycin, 83.3% (25/30), followed by amoxicillin, 53.3% (16/30) were the highest resisted virulence genes. The study demonstrated considerable isolation rate, multiple antimicrobial resistant isolates and high resistant virulent genes in diarrheic calves. It also indicated that the potential importance of calves as source of pathogenic E. coli strains and resistant genes for human diarrhea infection. Improving the hygienic practice of farms and wise use of antimicrobials could help to reduce the occurrence of pathogenic E. coli in farms. Hence, further studies are needed to describe all virulent factors and serotypes associated with the emergence of drug resistant pathogenic E. coli strains in calves.

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RETRACTED ARTICLE: Molecular detection of pathogenic Escherichia coli strains and their antibiogram associated with risk factors from diarrheic calves in Jimma Ethiopia

Ali

Tesema

Belachew

2021

Sci Rep

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Molecular characterisation of Shiga toxin-producing Escherichia coli O157:H7 isolates from cattle in eastern Turkey

Kalender¹,

Kılıç²

2016

Vet. Med. - Czech

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Shiga toxin-producing Escherichia coli (STEC) O157:H7 is an important foodborne pathogen worldwide. In Turkey, little is known about the molecular characteristics of STEC O157:H7 isolates. The aim of this study was to examine the genetic diversity of STEC O157:H7 isolates from cattle in Turkey using pulsed-field gel electrophoresis (PFGE). A total of 18 STEC O157:H7 strains were isolated from 540 rectal swab samples of slaughtered cattle in two abattoirs (coded A and B) located in eastern Turkey. The presence of virulence genes (stx1, stx2, hlyA and eae) was detected using the polymerase chain reaction. Of the 18 STEC O157:H7 isolates, 14 had stx2, hlyA, and eae genes. Four of the isolates were positive for stx1, stx2, hlyA, and eae genes. Thirteen different PFGE patterns were observed among the STEC O157:H7 isolates. The most common PFGE pattern was X1, which was detected in six STEC O157:H7 isolates. These six isolates contained the same virulence genes (stx2, eae and hlyA). However, some of the isolates containing the same virulence genes showed different PFGE profiles. Eleven different PFGE patterns were detected among the isolates from abattoir B, and two different PFGE patterns were detected among the isolates from abattoir A. The results of PFGE revealed a wide genetic diversity among the STEC O157:H7 isolates from cattle in Turkey. This is the first study on PFGE typing of STEC O157:H7 isolates from cattle in Turkey and further studies are needed.

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Investigating the presence and antibiotic susceptibilities of Escherichia coli O157 and Listeria monocytogenes in ruminant feces and feed in Balıkesir province

Babacan

2024

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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In this study, which was conducted for the first time in Balıkesir, it was purposed to determine the existense, virulence factors and antibiotic susceptibility of Escherichia coli O157, which is zoonotic in ruminant feces and feed, and the pathogen Listeria monocytogenes, which causes diseases in humans and animals, and to use these results as epidemiological data in our province, region and country. Feces and animal feed samples were analyzed simultaneously for in order of E. coli O157 and L. Monocytogenes according to ISO 16654: 2001 / Amd 1: 2017 and ISO 11290-1. 38 E.coli O157 was isolated from a total of one hunderd stool samples. 18 L. monocytogenes were isolated from a total of one hunderd stool samples. 6 L. monocytogenes were isolated from 50 silage samples. Three of these isolates were isolated from faeces and silage samples taken from the same farm with L. monocytogenes isolates isolated from sheep feces. E. coli O157 could not be isolated from a total of 100 silage and feed samples. All L. monocytogenes isolates were susceptible to sulfamethoxazole / trimethoprim, tetracycline, streptomycin, meropenem and erythromycin. The highest resistance was detected against Sulbactam / ampicillin. 3 E. coli O157 isolates were found resistant to Gentamicin and 7 isolates to Tobramycin. 21 isolates were resistant to erythromycin, and 12 isolates were intermediate. According to PCR results of fliCH7, Stx1, Stx2, eaeA and EhlyA genes, EhlyA gene was found in 20 E. coli O157 isolates. Of these isolates, 4 were isolated from sheep feces and 16 from calf feces. The stx1 gene was found in a total of 5 E. coli O157 isolates, one from sheep feces and four from calf feces. EhlyA gene was also found in all isolates with stx1 gene. The stx2 gene was found in a total of 3 E. coli O157 isolates, one from sheep dung and two from calf dung. Intimin gene was found in 8 E. coli O157 isolates, two of which are sheep faeces and six calf faecal isolates. EhlyA gene was found in all isolates with intimin gene. In this study, enterohaemolysin is the predominant virulence factor among the isolates. Epidemiologically, silage was thought to be the main source of L. monocytogenes contamination, and recently, silage contamination continued in Balikesir.

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Virulence genes of Shiga toxin-producing Escherichia coli O157:H7 strains isolated from calves and cattle

Cited by 3 publications

References 25 publications

RETRACTED ARTICLE: Molecular detection of pathogenic Escherichia coli strains and their antibiogram associated with risk factors from diarrheic calves in Jimma Ethiopia

RETRACTED ARTICLE: Molecular detection of pathogenic Escherichia coli strains and their antibiogram associated with risk factors from diarrheic calves in Jimma Ethiopia

Molecular characterisation of Shiga toxin-producing Escherichia coli O157:H7 isolates from cattle in eastern Turkey

Investigating the presence and antibiotic susceptibilities of Escherichia coli O157 and Listeria monocytogenes in ruminant feces and feed in Balıkesir province

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