Virulence mechanisms of Tannerella forsythia

Sharma, Ashu

doi:10.1111/j.1600-0757.2009.00332.x

Cited by 189 publications

(233 citation statements)

References 96 publications

(155 reference statements)

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“…The predicted structure of the BspA protein indicates that the protein contains LRR and bacterial immunoglobulin-like domains (Big_2) (Sharma, 2010). These domains have been implicated in protein-protein interactions and thus may be involved in receptor binding.…”

Section: Discussionmentioning

confidence: 99%

“…While the contribution of T. forsythia in periodontitis has been well established through epidemiological and clinical intervention studies, the mechanisms underlying T. forsythia virulence are poorly understood (Sharma, 2010). T. forsythia has been detected intracellularly in buccal and crevicular epithelium of patients with periodontitis (Colombo et al, 2007;Rudney et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

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Tannerella forsythia invasion in oral epithelial cells requires phosphoinositide 3-kinase activation and clathrin-mediated endocytosis

Mishima

Sharma

2011

Microbiology

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Tannerella forsythia, a Gram-negative anaerobe implicated in periodontitis, has been detected within human buccal epithelial cells and shown to invade oral epithelial cells in vitro. We have previously shown that this bacterium triggers host tyrosine kinase-dependent phosphorylation and actin-dependent cytoskeleton reorganization for invasion. On the bacterial side, the leucine-rich repeat cell-surface BspA protein is important for entry. The present study was undertaken to identify host signalling molecules during T. forsythia entry into human oral and cervical epithelial cells. Specifically, the roles of phosphatidylinositol 3-kinase (PI3K), Rho-family GTPases, cholesterol-rich membrane microdomains and the endocytic protein clathrin were investigated. For this purpose, cell lines were pretreated with chemical inhibitors or small interfering RNAs (siRNAs) that target PI3Ks, Rho GTPases, clathrin and cholesterol (a critical component of ‘lipid rafts’), and the resulting effects on T. forsythia uptake were determined. Our studies revealed that T. forsythia entry is dependent on host PI3K signalling, and that purified BspA protein causes activation of this lipid kinase. Bacterial entry also requires the cooperation of host Rac1 GTPase. Finally, our findings indicate an important role for clathrin and cholesterol-rich lipid microdomains in the internalization process

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Tannerella forsythia invasion in oral epithelial cells requires phosphoinositide 3-kinase activation and clathrin-mediated endocytosis

Mishima

Sharma

2011

Microbiology

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“…Consistent with this T. denticola has also been designated as a keystone pathogen. Excellent reviews, too many to list here, are available detailing the roles that P. gingivalis, T. denticola and other bacteria play in periodontal disease (Darveau 2010;Dashper et al 2010;How et al 2016;McDowell et al 2012;Mysak et al 2014;Podzimek et al 2015;Sharma 2010). …”

Section: Expansion Of the Bacterial Complex Conceptmentioning

confidence: 99%

Gene Regulation, Two Component Regulatory Systems, and Adaptive Responses in Treponema Denticola

Marconi

2017

Current Topics in Microbiology and Immunology

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“…P. gingivalis, T. forsythia and T. denticola were frequently co-isolated from lesion of periodontitis [5]. T. denticola and T. forsythia have fastidious growth requirements for culture [6,11]. Several bacterial pairs were reptorted to required cell to cell contact for enhancement of biofilm formation [41,42].…”

Section: Discussionmentioning

confidence: 99%

“…forsythia is also an important and consistent member of the microbial biofilm in periodontal disease and possessed several potential pathogenic factors such as Bacteroides surface protein A (BspA) [11]; however, there is limited information regarding its colonization strategy.…”

Section: Introductionmentioning

confidence: 99%

Dentilisin involvement in coaggregation between Treponema denticola and Tannerella forsythia

et al. 2014

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Periodontitis arises from a biofilm consisting of gram-negative anaerobic rods and spirochetes. Porphyromonas gingivalis, Treponema denticola, and Tannerella forsythia, termed the Red complex, have been co-isolated with high frequency from chronic periodontitis lesions, and these microorganisms are thought to be major pathogens of the disease. Coaggregation is an important strategy in the colonization of dental plaque biofilm by these bacteria. In the present study, we investigated the coaggregation of T. denticola strains with T. forsythia ATCC 43037 by use of visual grading or spectrophotometry. T. denticola ATCC 35405 coaggregated with T. forsythia, reaching a plateau at approximately 60 min. This coaggregation was inhibited by heat treatment of T. denticola ATCC 35405, but not of T. forsythia. Disaccharides such as sucrose, maltose, and lactose inhibited coaggregation by approximately 50%. The coaggregation reaction varied among T. denticola strains. There was somewhat less coaggregation between T. denticola ATCC 33520 and T. forsythia than between T. denticola ATCC 35405 and T. forsythia, although this difference was not statistically significant; T. denticola ATCC33521 showed a trace level of coaggregation with T. forsythia. The magnitude of coaggregation among the three T. denticola strains was proportional to their dentilisin activities. Inactivation of dentilisin abolished coaggregation activity, but inactivation of the major outer sheath protein did not. Phenylmethylsulfonyl fluoride (PMSF) also did not affect coaggregation. These results indicate that dentilisin is involved indirectly in the coaggregation between T. denticola and T. forsythia, because its proteolytic activity is not required, possibly via ligand maturation.2

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Virulence mechanisms of Tannerella forsythia

Cited by 189 publications

References 96 publications

Tannerella forsythia invasion in oral epithelial cells requires phosphoinositide 3-kinase activation and clathrin-mediated endocytosis

Tannerella forsythia invasion in oral epithelial cells requires phosphoinositide 3-kinase activation and clathrin-mediated endocytosis

Gene Regulation, Two Component Regulatory Systems, and Adaptive Responses in Treponema Denticola

Dentilisin involvement in coaggregation between Treponema denticola and Tannerella forsythia

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