Virulence of Chick Embryo Fibroblast‐Passaged Flury HEP Rabies Virus and Its Revertants in Mice

Arai, Yohko T.

doi:10.1111/j.1348-0421.1985.tb00884.x

Cited by 9 publications

(3 citation statements)

References 17 publications

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“…Los neonatos podrían actuar como amplificadores del VR; se ha observado que dosis subletales para los adultos pueden afectar a los lactantes dada su alta susceptibilidad, además de que cepas vacunales atenuadas, inocuas para adultos, pueden enfermar a los lactantes. 11 Así, podríamos establecer un mecanismo hipotético de preservación del VR en una población reservorio, en el cual los neonatos amplificarían bajas dosis de virus y podrían diseminarlo con mayor éxito entre congéneres susceptibles, en un proceso representado en la Figura 1. Apoyando esta hipótesis está el hecho de que en los quirópteros la prevalencia de anticuerpos contra los Lyssavirus coincide con la época reproductiva.…”

Section: Discussionunclassified

Transmisión vertical del virus de la rabia cría-madre, fenómeno que podría mantener al virus en especies reservorios de vida silvestre

Aréchiga-Ceballos

Almazán-Marín

Aguilar-Setién

2019

GMM

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el virus de la rabia consiste en inocular vía intracraneal ratones lactantes para detectar signos de rabia en un periodo de 21 días. Objetivo: Constatar el contagio viral en las madres de ratones lactantes inoculados con virus de la rabia. Método: Veintisiete aislados mexicanos de virus de la rabia se inocularon vía intracraneal en ratones lactantes, los cuales fueron observados por 21 días y sus madres, por 60 días. El diagnóstico se llevó a cabo mediante inmunofluorescencia en cerebro. El virus se caracterizó por secuenciación y anticuerpos monoclonales. Resultados: Todas las camadas presentaron rabia entre siete y 15 días posinoculación (p. i.); tres de las 27 hembras (11 %), a los días 33, 37 y 39 p. i. de sus crías. La caracterización viral mostró que las madres se infectaron con la misma variante de sus crías, dos procedían de murciélago hematófago y una de perro. Las camadas que trasmitieron rabia a sus madres fueron nueve individuos. Conclusiones: En la naturaleza, el virus de la rabia podría preservarse mediante la transmisión de los neonatos (más susceptibles de contraer y amplificar el virus) a sus madres.

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Section: Discussionunclassified

Transmisión vertical del virus de la rabia cría-madre, fenómeno que podría mantener al virus en especies reservorios de vida silvestre

Aréchiga-Ceballos

Almazán-Marín

Aguilar-Setién

2019

GMM

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“…passages in foxes and mice, respectively. It was shown that the consensus strain of SAD B19 was extremely stable after serial passages in vivo , in contrast to some other attenuated rabies virus strains that can revert to more pathogenic variants by a few passages in mouse brain (8).…”

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confidence: 99%

Genetic stability (in vivo) of the attenuated oral rabies virus vaccine SAD B19

Beckert

Geue

Vos

et al. 2009

Microbiology and Immunology

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The distribution of oral rabies vaccine baits containing replication-competent live viruses poses certain environmental safety risks; among others, the possibility of reversion to or an increase in virulence. Hence, the genetic stability of the complete genome of the most widely used oral rabies vaccine virus, SAD B19, was examined after four and 10 serial i.c. passages in foxes and mice, respectively. It was shown that the consensus strain of SAD B19 was extremely stable in vivo. After 10 consecutive passages in mice not a single mutation was observed. In foxes, seven single nucleotide exchanges were found between the first and fourth passage, of which only one resulted in an amino acid exchange at position 9240 of the L-gene. This mutation was not observed during the first three passages and, furthermore, it was shown that this mutation was not linked to enhanced virulence.

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“…Neonates might act as RV amplifiers; sub-lethal doses for adults have been observed to be able to affect infants given their high susceptibility, in addition to the fact that attenuated vaccine strains, which are harmless to adults, can make infants sick. 11 Thus, we might establish a hypothetical mechanism of RV preservation in a reservoir population, where neonates would amplify low doses of the virus and could disseminate it with greater success among susceptible congeners, in a process depicted in Figure 1. Supporting this hypothesis is the fact that, in Chiroptera, the prevalence of antibodies against Lyssavirus coincides with the mating season.…”

Section: Discussionmentioning

confidence: 99%

Rabies virus litter-to-mother vertical transmission, a phenomenon that could preserve the virus in wildlife reservoirs

Aréchiga-Ceballos¹,

Almazán-Marín²,

Aguilar-Setién³

2023

GMM

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Introduction:The biological test established by the World Health Organization to isolate and amplify the rabies virus consists in inoculating lactating mice by intracranial route and detecting rabies signs for 21 days. Objective: To verify viral transmission in mothers of rabies virus-inoculated lactating mice. Method: Twenty-seven Mexican rabies virus isolates were inoculated by intracranial route in lactating mice, which were observed for 21 days. The mothers were observed for 60 days. The diagnosis was established by immunofluorescence in brain tissue. The virus was characterized by sequencing and with monoclonal antibodies. Results: All litters showed rabies at between 7 and 15 days post-inoculation (p.i.). Three of the 27 females (11 %) had developed rabies at days 33, 37 and 39 p.i. of their litters. Viral characterization showed that the mothers were infected with the same variant of their offspring, two of them stemming from hematophagous bat and one from dog. The litters that transmitted rabies to their mothers were nine individuals. Conclusions: In nature, the rabies virus could be preserved by transmission from neonates (more susceptible to contracting and amplifying the rabies virus) to their mothers.

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Virulence of Chick Embryo Fibroblast‐Passaged Flury HEP Rabies Virus and Its Revertants in Mice

Cited by 9 publications

References 17 publications

Transmisión vertical del virus de la rabia cría-madre, fenómeno que podría mantener al virus en especies reservorios de vida silvestre

Transmisión vertical del virus de la rabia cría-madre, fenómeno que podría mantener al virus en especies reservorios de vida silvestre

Genetic stability (in vivo) of the attenuated oral rabies virus vaccine SAD B19

Rabies virus litter-to-mother vertical transmission, a phenomenon that could preserve the virus in wildlife reservoirs

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