Virulence of Enterococcus Species Isolated from the Nile Tilapia and Human: Evaluation of MALDI-TOF-MS Diagnostic Techniques Accuracy in Comparison to PCR

al., Mohamed E. Enany et

doi:10.21608/ejabf.2022.234268

Cited by 3 publications

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…faecalis and E . faecium ) are widely distributed in farmed tilapia and are a cause of sepsis, urinary tract infections, and wounds in humans [ 33 ]. According to Regulation (EC) No 852/2004 [ 34 ], food business operators are required to comply with microbiological criteria.…”

Section: Discussionmentioning

confidence: 99%

Enterococcus spp. in fish: Analysis of the presence and resistance in samples from Tri-City, Poland

Kukułowicz,

Steinka,

Gardocka

2024

PLoS ONE

View full text Add to dashboard Cite

The growing concern over antibiotic resistance in foodborne pathogens necessitates comprehensive assessments of its prevalence and associated risks in various food products. The present study aimed to assess the occurrence of Enterococcus spp. in samples of fish purchased at various points of sale in the Tricity region. The selection of products (n = 74) was based on their availability and included both fish caught in the Baltic region and products imported from, Vietnam, China, Norway, and European Union (EU) countries. For bacterial isolation, samples were inoculated into selective broth, and the growth of enterococci was assessed based on turbidity. Positive cultures were confirmed by a change in color in bromocresol purple broth and were isolated on Slanetz-Bartley agar. Bacteria were present in all tested samples regardless of the degree of raw material processing as follows: frozen (F)– 55%, fresh/raw (FS)– 70.6%, thawed (DF)– 30%, smoked (S)– 50%, and the packaging methods, modified atmosphere packaging (MAP)– 34.4%, unit packaging (UP)– 75%, and sold in bulk (SB)– 76.9%, with an overall frequency of occurrence of 58.1%. The number of bacteria ranged from not detected to 4.28-log cfu/g, with the lowest mean values for thawed fish and those packed in MAP. Tests conducted on 24 strains isolated from samples showed their varied sensitivity to tetracyclines. Single cases of multidrug resistance of the tested strains were also observed. The conducted statistical analysis did not show statistically significant differences in the count of enterococci based on the origin, degree of processing, or packaging (p < 0.05). Moreover, differences in strain sensitivity to ampicillin were observed. Detected cases of resistance, especially to tetracycline, require careful monitoring and action to limit the health risks associated with resistant bacterial strains in food products.

show abstract

Section: Discussionmentioning

confidence: 99%

Enterococcus spp. in fish: Analysis of the presence and resistance in samples from Tri-City, Poland

Kukułowicz,

Steinka,

Gardocka

2024

PLoS ONE

View full text Add to dashboard Cite

show abstract

Dissemination, virulence characteristic, antibiotic resistance determinants of emerging linezolid and vancomycin-resistant Enterococcus spp. in fish and crustacean

Abdel-Raheem,

Khodier,

Almathen

et al. 2024

International Journal of Food Microbiology

View full text Add to dashboard Cite

Phenotypic and genotypic characterization of Enterococcus faecalis and Enterococcus faecium isolated from fish, vegetables, and humans

Mubarak,

El-Zamkan,

Younis

et al. 2024

Sci Rep

View full text Add to dashboard Cite

Enterococci, common hospital-acquired infections in immunocompromised patients, have garnered attention in clinical microbiology. To determine the clinical relevance of enterococci as food-borne pathogens, 116 fish, 90 vegetables, and 120 human diarrheal samples were tested for E. faecalis and E. faecium pathogenicity. Conventionally, 69 of 326 (21.17%) samples were positive for Enterococcus species, 52 (15.95%) of which were molecularly classified as E. faecalis and 13 (3.99%) as E. faecium. The E. faecalis contamination percentage of fresh fish (19.70%) was higher than frozen fish (4%). Cauliflower had the highest E. faecalis percentage (16.67%) when fish and vegetable samples didn’t harbor the E. faecium atpA gene. 23.33% and 10.83% of participants’ samples were molecularly confirmed as E. faecalis and E. faecium positive, respectively. E. faecalis isolates had all virulence genes, with gels being the most common (65.38%), while cylA and asa1 genes couldn’t be detected in E. faecium isolates. E. faecalis showed the highest resistance against vancomycin and tetracycline (69.23%), whereas E. faecium extremely resisted tetracycline (76.92%) and erythromycin (69.23%) with the recognition of MDR among 44.2% of E. faecalis and 38.5% of E. faecium isolates. The great similarity of our isolates showed the clinical importance of food-borne antibiotic-resistant enterococci.

show abstract

Virulence of Enterococcus Species Isolated from the Nile Tilapia and Human: Evaluation of MALDI-TOF-MS Diagnostic Techniques Accuracy in Comparison to PCR

Cited by 3 publications

References 0 publications

Enterococcus spp. in fish: Analysis of the presence and resistance in samples from Tri-City, Poland

Enterococcus spp. in fish: Analysis of the presence and resistance in samples from Tri-City, Poland

Dissemination, virulence characteristic, antibiotic resistance determinants of emerging linezolid and vancomycin-resistant Enterococcus spp. in fish and crustacean

Phenotypic and genotypic characterization of Enterococcus faecalis and Enterococcus faecium isolated from fish, vegetables, and humans

Contact Info

Product

Resources

About