Virulence of entomopathogenic nematodes to larvae of the guava weevil, Conotrachelus psidii (Coleoptera: Curculionidae), in laboratory and greenhouse experiments

Dolinski, Cláudia; Valle, Eleodoro Del; Stuart, Robin J.

doi:10.1016/j.biocontrol.2005.12.014

Cited by 31 publications

(20 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Both isolates which brought about the highest rates of mortality in this experiment are from the family Heterorhabditidae, fact also observed in other studies performed with C. sordidus or other Curculionidae (treverrOw;BeDDinG, 1993;DOlinsKi et al, 2006;sepúlveDa-canO et al, 2008;GiOMetti et al, 2011;MwaitulO et al, 2011). All the cited authors deduce that the greatest susceptibility to Heterorhabditis is probably related to the greatest facility of these ones to enter into the insect´s body, in relation to the Steinernema, once they are comparatively smaller and they have a capacity to pierce the cuticle, and their entrance is not restricted to the natural openings of the insect, what is important in the cases in which it is focused the control of adults which are more resistant to the EPNs than larvae (MwaitulO et al, 2011).…”

Section: Arqsupporting

confidence: 85%

Entomopathogenic nematodes and their interaction with chemical insecticide aiming at the control of banana weevil borer, Cosmopolites Sordidus Germar (Coleoptera: Curculionidae)

Bortoluzzi

Alves

et al. 2013

Arq. Inst. Biol.

View full text Add to dashboard Cite

The banana weevil borer (Cosmopolites sordidus) is the main pest of banana crops, causing significant losses in productivity, being recommended control by chemical insecticides which cause several environmental impacts. On the other hand, entomopathogenic nematodes can be an alternative to the pest control, mainly because of their habits. Thus, this study aimed at evaluating isolated entomopathogenic nematodes under laboratory conditions and also their interaction with a chemical insecticide (carbofuran), aiming at their use for the weevil borer control. Sixteen Sterinernematidae and Heterorhabditidae isolates were evaluated, applied over the banana tree pseudo stem (100 JIs/cm 2 ) and they were compared to one another concerning mortality caused in adults individual of C. sordidus. The most infective isolates were subjected to in vivo multiplication at the host Galleria mellonella and interaction with the insecticide carbofuran, including in this case, viability and infectivity analysis of the entomopathogenic nematodes exposed to the product, as well as the effect of the insecticide on the symbiotic bacteria of the entomopathogenic nematodes. The experiments at this stage were conducted in completely randomized design and the data were subjected to ANOVA, with application of the Tukey test (p < 0.05). The most virulent isolates were IBCBn24 and IBCBn40 (respectively 33.3% and 36.7% of confirmed mortality), which also showed high multiplication in corpses. The insecticide did not affect the viability of the isolate, but it really harmed its infectivity, although it did not affect the development of the symbiotic bacterium.KEY WORDS: Heterorhabditis, Steinernema, biological control, chemical insecticide. RESUMONEMATOIDES ENTOMOPATOGÊNICOS E SUA INTERAÇÃO COM INSETICIDA QUÍMI-CO VISANDO AO CONTROLE DA BROCA-DA-BANANEIRA COSMOPOLITES SORDIDUS GERMAR (COLEOPTERA: CURCULIONIDAE). A broca-da-bananeira (Cosmopolites sordidus) é a principal praga dos cultivos de banana, acarretando perdas significativas na produtividade da cultura, sendo recomendados inseticidas químicos para seu controle, os quais causam impacto ambiental. Por outro lado, os nematoides entomopatogênicos podem ser uma alternativa para o controle da praga, principalmente devido aos seus hábitos. Assim, este trabalho teve como objetivo avaliar isolados de nematoides entomopatogênicos em condições de laboratório e a interação com inseticida químico (carbofurano), visando a sua utilização no controle da broca. Foram testados 16 isolados das famílias Sterinernematidae e Heterorhabditidae, aplicados sobre pseudocaule de banananeira (100 JIs/cm 2 ) e comparados entre si quanto à mortalidade causada em indivíduos adultos de C. sordidus. Os isolados mais infectivos foram submetidos a experimentos de multiplicação in vivo no hospedeiro Galleria mellonella e de interação com o inseticida carbofurano incluindo, neste caso, análise de viabilidade e infectividade dos nematoides expostos ao produto, bem como o efeito do inseticida sobre as bactérias simbionte...

show abstract

Section: Arqsupporting

confidence: 85%

Entomopathogenic nematodes and their interaction with chemical insecticide aiming at the control of banana weevil borer, Cosmopolites Sordidus Germar (Coleoptera: Curculionidae)

Bortoluzzi

Alves

et al. 2013

Arq. Inst. Biol.

View full text Add to dashboard Cite

show abstract

“…The two EPN species were multiplied in last-instar Galleria mellonella L. (Lepidoptera: Pyralidae) larvae. G. mellonella larvae were reared in the laboratory as described by Dolinski et al [24] . These larvae were placed in 9-cm Petri dishes lined with two sheets of filter paper moistened with 2 mL of a nematode suspension containing 200 IJs.…”

Section: Maintenance Of Entomopathogenic Nematodes and Nematophagous mentioning

confidence: 99%

Studies on the interaction between tropical nematophagous fungi and entomopathogenic nematodes (Rhabditida) under laboratory conditions

Arêdes¹,

Ribeiro²,

Dias³

et al. 2017

Nematoda

Self Cite

View full text Add to dashboard Cite

HIGHLIGHTS• Arthrobotrys musiformis AM4 and Arthrobotrys sp. CO7 were the most aggressive tropical fungi tested againstHeterorhabditis indica LPP30.• Water agar medium provided the best conditions to test nematode-fungus interaction with lowest environmental impact among the six media tested.• H. indica LPP30 was more apprehended than S. carpocapsae All by both tropical fungi tested.• In a soil environment, there was a repellent action from Arthrobotrys sp. CO7 against S. carpocapsae All.ABSTRACT: This study settles a step toward to understanding the interaction between nematophagous fungi (NFs) generally found in the soil and entomopathogenic nematodes (EPNs) used in the control of agricultural pests. There are very few studies linking EPNs and their natural enemies around the world and none in Brazil. Therefore, we decided to test differences regarding aggressiveness and susceptibility among the fungi and EPNs found and used in this country. The aim of this work was to study the interactions between the tropical native NFs, Arthrobotrys oligospora AM2, Arthrobotrys musiformis AM4, Arthrobotrys sp. CO7, Arthrobotrys sp. CC7 and the EPNs, Heterorhabditis indica LPP30 (Heterorhabditidae), a native nematode, and Steinernema carpocapsae All (Steinernematidae), a exotic nematode, under laboratory conditions. The interaction was studied in four aspects: 1) NF aggressiveness against EPNs; 2) effect of culture medium on NF aggressiveness to infective juveniles (IJs); 3) EPN susceptibility to NFs; and 4) dispersion of IJs in two types of soil in the presence of NFs. The fungi A. musiformis AM4 and Arthrobotrys sp. CO7 were considered the most aggressive among the tested strains, since they trapped more IJs. Water agar medium provided the best conditions to test nematode-fungus interaction among the six media tested, since the fungi tested produced more trapping structures to apprehend IJs on it. Both fungi were considered very aggressive to EPNs due to their apprehension capacity. Regarding susceptibility, H. indica LPP30 IJs were more susceptible than IJs of S. carpocapsae ALL to both A. musiformis AM4 and Arthrobotrys sp. CO7 in the first 24 h of contact. However, after 48 h, the means of IJs captured by the fungi became statistically equal, except for S. carpocapsae All in Assay 2, which presented the highest mean capture rate. The fungus Arthrobotrys sp. CO7 repelled S. carpocapsae All IJs in sand at 15 cm. The more we learn about biotic factors that interfere in biocontrol, the more effective we can make it. Future studies will focus on the characterization of these interactions in the field under tropical conditions.

show abstract

“…Heterorhabditis baujardi LPP7 Laboratory and greenhouse Dolinski et al [13] Fungus gnat Bradysia mabiusi…”

Section: Psidium Guajavamentioning

confidence: 99%

“…In greenhouse experiments, the application of aqueous suspensions has been found to be effective against Conotrachelus psidii Marshall (Coleoptera: Curculionidae) (guava weevil) [13] , Mahanarva spectabilis (Distant) (Hemiptera: Cercopidae) (spittlebug) [86] , Diabrotica speciosa (Germar) (leaf beetle) [25] , Sphenophorus levis Vaurie (Coleoptera: Curculionidae) (sugarcane weevil) [16] and Dysmicoccus texensis (Tinsley) (Hemiptera, Pseudococcidae) [19] . With respect to field trials, good results have been obtained against M. fimbriolata (sugarcane root spittlebug) [11] , C. psidii [13] , Ceratitis capitata (Wiedemann) (Diptera: Tephritidae) (fruit fly), Anastrepha fraterculus (Wied.) (Diptera: Tephritidae) (South American fruit fly), and D. texensis [20] .…”

Section: Applicationmentioning

confidence: 99%

“…This group has investigated the potential of EPNs to manage C. psidii, commonly known as the guava weevil. Initially, laboratory studies were conducted to ascertain the most virulent strains [13] , followed by greenhouse tests and field trials with application of EPNs using the insect cadaver technique [97] . Based on these results, with confirmation of the efficiency of the EPNs, a partnership was established with a group of about 20 farmers organized in an association called GOIACAM (Association of Guava Producers of Cachoeiras de Macacu).…”

Section: Successful Use Of Entomopathogenic Nematodes In Brazilmentioning

confidence: 99%

See 1 more Smart Citation

Studies on entomopathogenic nematodes in Brazil: past and future

Dolinski¹,

Monteiro²,

Andaló³

et al. 2014

Nematoda

Self Cite

View full text Add to dashboard Cite

HIGHLIGHTS• Lauro Travassos in 1927 created genus Steinernema.• Two new species of entomopathogenic nematodes were described.• Studies on ecology, biology, production, formulation and application of native species have been carried out since 2000. ABSTRACT:In Brazil, the first study with entomopathogenic nematodes (EPNs) was conducted by Lauro Travassos in 1927, when he created the genus Steineria, that latter was changed to Steinernema. After important meetings and courses, there was a great development of EPNs research in various states by different research groups in Brazil. Many EPN species were isolated throughout the country and two new species described: Heterorhabditis amazonensis and Steinernema brazilense. The behavioral aspects, life cycle and pathogenicity of native EPNs were analyzed. Also their persistence and survival in the soil and the biotic and abiotic factors that may affect their efficiency as biocontrol agents. Due to the particular native species characteristics, production, formulation and application technologies were developed for them. Here in we summarize main Brazilians studies in different areas using EPNs. This is the first review on the subject in Brazil.Keywords: biology, ecology, behavior, production, formulation.Cite as Dolinski C, Monteiro C, Andaló V, Leite LG. Studies on entomopathogenic nematodes in Brazil: past and future. Nematoda. 2017;4:e102017. http://dx

show abstract

Virulence of entomopathogenic nematodes to larvae of the guava weevil, Conotrachelus psidii (Coleoptera: Curculionidae), in laboratory and greenhouse experiments

Cited by 31 publications

References 15 publications

Entomopathogenic nematodes and their interaction with chemical insecticide aiming at the control of banana weevil borer, Cosmopolites Sordidus Germar (Coleoptera: Curculionidae)

Entomopathogenic nematodes and their interaction with chemical insecticide aiming at the control of banana weevil borer, Cosmopolites Sordidus Germar (Coleoptera: Curculionidae)

Studies on the interaction between tropical nematophagous fungi and entomopathogenic nematodes (Rhabditida) under laboratory conditions

Studies on entomopathogenic nematodes in Brazil: past and future

Contact Info

Product

Resources

About