Virulence of Raffaelea quercivora isolates inoculated into Quercus serrata logs and Q. crispula saplings

Kusumoto, Dai; Masuya, Hayato; Ohmura, Keiji; Kamata, Naoto

doi:10.1007/s10310-011-0302-5

Cited by 12 publications

(9 citation statements)

References 12 publications

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“…These results suggest that the effect of inoculation with RA67 on susceptible Q. crispula trees was primarily a consequence of wounding rather than of fungal virulence. Previously, the virulence of R. quercivora to host trees differed among isolates based on the extent of discoloration (Kusumoto et al 2012(Kusumoto et al , 2014(Kusumoto et al , 2015. However, the non-conductive sapwood appears to form earlier than the discoloration (Murata et al 2009).…”

Section: Discussionmentioning

confidence: 99%

“…In fact, the pathogenicity of R. quercivora to Q. crispula and Q. serrata was demonstrated by death of the trees after multiple-inoculation tests Murata et al 2005). Moreover, the fungal virulence against Japanese fagaceous species, including Q. crispula, appears to differ among the isolates collected from Japan (Kusumoto et al 2012) and other Asian countries (Kusumoto et al 2014(Kusumoto et al , 2015 based on comparisons of the widths of discoloured tissue after single-inoculation tests with R. quercivora. However, no studies have evaluated the virulence of the fungus in terms of the wilting mechanism.…”

Section: Introductionmentioning

confidence: 99%

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Variations in virulence and hyphal growth of four Raffaelea quercivora isolates within Quercus crispula

et al. 2016

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Mass mortality of fagaceous trees caused by Japanese oak wilt has occurred widely in Japan. Although virulence of the causal fungus, Raffaelea quercivora, appeared to differ among isolates, its relation to the fungal growth within trees was unknown. To clarify the differences in fungal virulence against susceptible Quercus crispula, we examined fungal growth of four R. quercivora isolates within trees and the resulting virulence. In our study, the isolates were multiple-inoculated in seedlings and single-inoculated in twigs of mature trees. In the multiple-inoculation test, mortality rates were examined by the observation of external symptoms. In the single-inoculation test, water conductance and hyphal growth within the trees were examined by applying aqueous dyes and fluorescence microscopy, respectively. Mortality rates, the proportion of the cross-sectional area comprising non-conductive sapwood and horizontal hyphal growth differed significantly among the isolates. Univariate logistic regression analyses showed that both the proportion of non-conductive sapwood and hyphal growth were significantly positively related to mortality rates. For three isolates, hyphal growth was significantly positively correlated with the proportion of non-conductive sapwood. These results suggested that the virulence against Q. crispula varies among R. quercivora isolates and that the extent of fungal colonization of the tree determines fungal virulence.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Variations in virulence and hyphal growth of four Raffaelea quercivora isolates within Quercus crispula

et al. 2016

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“…These differences in discoloration may relate to the lower mortality levels of evergreen oaks in the field, because most R. quercivora isolates used in our previous studies were categorized as having low virulence (16,17). The sapwood discoloration widths of these species were comparatively smaller than the deciduous ring-porous Quercus spp.…”

Section: Discussionmentioning

confidence: 86%

“…However, our understanding about tree-fungus interactions remains limited compared with tree-beetle interactions. Furthermore, Kusumoto et al (16,17) collected fungal isolates from Japan and other Asian countries and showed that the most virulent isolate caused twice as much discoloration in Q. serrata logs than the least virulent isolate. In addition, the authors suggested that the xylem vessel arrangement in fagaceous trees (i.e., ring-, radial-, and diffuse-porous wood) is associated with differences in the tangential spread of sapwood discoloration among host species.…”

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confidence: 99%

Comparison of Sapwood Discoloration in Fagaceae Trees After Inoculation with Isolates of Raffaelea quercivora, Cause of Mass Mortality of Japanese Oak Trees

et al. 2015

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“…Phytophthora cinnamomi (sudden oak death) (Brasier, 1996;Juzwik , 2011), Raffaelea quercivora (Kusumoto , 2012;Kubono Ito, 2002;Murata , 2005;2007 (Kim, 2009;Korea Forest Research Institute, 2010). (Platypus koryoensis) Raffaelea sp.…”

Section: Ceratocystis Fagacearum (Oak Wilt)mentioning

confidence: 99%

Assessment of Virulence of Raffaelea quercus-mongolicae and Raffaelea spp. Isolates by Artificial Inoculation of Quercus mongolica Logs

Son¹,

Seo²,

Park³

2014

Research in Plant Disease

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The objective of this study was to evaluate the virulence of geographically different isolates of oak wilt pathogen, Raffaelea quercus-mongolicae and other Raffaelea species. In this study, mature trees of Quercus mongolica were inoculated with the various isolates of Raffaelea spp. and their virulence was evaluated by measuring the extent of sapwood discoloration resulting from the inoculation. The average length of discolored sapwood in a lateral direction was longest in the trees inoculated with the isolates from Korea (8.69 cm) followed by R. quercivora (7.51 cm) and the other Raffaelea spp. (3.35 cm). The lateral length of discolored sapwood caused by the inoculation with Korean strains varied from 4.71 to 14.90 cm indicating their differences in virulence. The area of discolored sapwood caused by the inoculation with Raffaelea spp. varied from 1.57 to 8.42 cm 2 indicating their differences in virulence. Based on the length and area of the discolored sapwoods, isolated YY and wj43 appeared to have the highest virulence among all the Raffaelea isolates tested. Each of the two isolates was obtained from Gangwon Province and Jeonbuk Province, respectively.

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Virulence of Raffaelea quercivora isolates inoculated into Quercus serrata logs and Q. crispula saplings

Cited by 12 publications

References 12 publications

Variations in virulence and hyphal growth of four Raffaelea quercivora isolates within Quercus crispula

Variations in virulence and hyphal growth of four Raffaelea quercivora isolates within Quercus crispula

Comparison of Sapwood Discoloration in Fagaceae Trees After Inoculation with Isolates of Raffaelea quercivora, Cause of Mass Mortality of Japanese Oak Trees

Assessment of Virulence of Raffaelea quercus-mongolicae and Raffaelea spp. Isolates by Artificial Inoculation of Quercus mongolica Logs

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