Virulence of oral Fusobacterium nucleatum from humans and non-human primates in mice

Júnior, Élerson Gaetti-Jardim; Luvizotto, Maria Cecília Rui; Ávila-Campos, Mário Júlio

doi:10.1590/s1517-83822000000200016

Cited by 5 publications

(3 citation statements)

References 33 publications

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“…Besides higher bacterial counts in periodontal diseased sites, we found more virulent bacterial strains. In addition, isolates from healthy subjects also showed pathogenic activity (17). …”

Section: Discussionmentioning

confidence: 99%

Periodontal profile and presence of periodontal pathogens in young African-Americans from Salvador, BA, Brazil

Victor¹,

Cortelli²,

Aquino³

et al. 2008

Braz. J. Microbiol.

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This cross-sectional study evaluated the periodontal status and the presence of periodontopathogens in 132 young, black ethnic subjects who live in Salvador/Bahia-Brazil and have never smoked. Periodontal Probing Depth (PPD), Clinical Attachment Level (CAL), Plaque Index (PI) and Gingival Index (GI) were measured and analyzed by ANOVA and Wilcoxon tests (p<0.05) according to gender and age. The presence of A.actinomycetemcomitans, P.gingivalis, E.corrodens and F.nucleatum was determined by PCR and was analyzed by ANOVA, Wilcoxon, Student-t tests (p<0.05). Mean values of PPD and CAL were 2.18 and 1.0mm, respectively. Clinical parameters did not show differences between subjects of varying gender and age. The microbial prevalence was observed to be 95.45% for E.corrodens followed by F.nucleatum with 68.18%, A.actinomycetemcomitans with 45.45% and P gingivalis with 40.9%. An association between the presence of pathogens and gender and age was not observed (p<0.05). PPD, CAL and PI were not associated with P.gingivalis; however, GI appeared in higher frequencies among subjects without P.gingivalis. In this young, black ethnic, Brazilian population, a high percentage (96.96%) of subjects harbored at least one selected periodontal pathogen, but most subjects showed a healthy periodontal status. Further investigations are required to evaluate the actual influence of the presence of these bacterial species.

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“…Besides higher bacterial counts in periodontal diseased sites, we found more virulent bacterial strains. In addition, isolates from healthy subjects also showed pathogenic activity (17). …”

Section: Discussionmentioning

confidence: 99%

Periodontal profile and presence of periodontal pathogens in young African-Americans from Salvador, BA, Brazil

Victor¹,

Cortelli²,

Aquino³

et al. 2008

Braz. J. Microbiol.

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“…To ensure log-phase growth, bacteria were passaged at least once before use in experiments. Previous literature determined that a 0.1 absorbance at 600 nm of Treponema denticola [ 34 ], Porphyromonas gingivalis [ 37 , 38 ], or Fusobacterium nucleatum [ 39 ] suspension contains 2.4 × 10 8 CFU/mL, 2.4 × 10 8 CFU/mL, and 1 × 10 8 CFU/mL of each of the bacteria, respectively. Purity of the cultures was confirmed by 16S sequencing prior to their use in the experiments.…”

Section: Methodsmentioning

confidence: 99%

Nisin and Nisin Probiotic Disrupt Oral Pathogenic Biofilms and Restore Their Microbiome Composition towards Healthy Control Levels in a Peri-Implantitis Setting

et al. 2022

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Peri-implantitis is characterized by chronic inflammation of the peri-implant supporting tissues that progressively and irreversibly leads to bone loss and, consequently, implant loss. Similar to periodontal disease, oral dysbiosis is thought to be a driver of peri-implantitis. However, managing peri-implantitis with traditional treatment methods, such as nonsurgical debridement or surgery, is not always successful. Thus, novel strategies have been proposed to address these shortcomings. One strategy is the use of probiotics as antimicrobial agents since they are considered safe for humans and the environment. Specifically, the probiotic Lactococcus lactis produces nisin, which has been used worldwide for food preservation. The objective of this study was to determine whether nisin and the wild-type (WT) nisin-producing L. lactis probiotic can disrupt oral pathogenic biofilms and promote a healthier oral microbiome within these oral biofilms on titanium discs. Using confocal imaging and 16S rRNA sequencing, this study revealed that nisin and WT L. lactis probiotic disrupt oral pathogenic biofilms in a peri-implantitis setting in vitro. More specifically, nisin decreased the viability of the pathogen-spiked biofilms dose-dependently from 62.53 ± 3.69% to 54.26 ± 3.35% and 44.88 ± 2.98%, respectively. Similarly, 105 CFU/mL of WT L. lactis significantly decreased biofilm viability to 52.45 ± 3.41%. Further, both treatments shift the composition, relative abundance, and diversity levels of these biofilms towards healthy control levels. A total of 1 µg/mL of nisin and 103 CFU/mL of WT L. lactis were able to revert the pathogen-mediated changes in the Proteobacteria (from 80.5 ± 2.9% to 75.6 ± 2.0%, 78.0 ± 2.8%, and 75.1 ± 5.3%, respectively) and Firmicutes (from 11.6 ± 1.6% to 15.4 ± 1.3%, 13.8 ± 1.8%, and 13.7 ± 2.6%, respectively) phyla back towards control levels. Thus, nisin and its nisin-producing L. lactis probiotic may be useful in treating peri-implantitis by promoting healthier oral biofilms, which may be useful for improving patient oral health.

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“…Previously, others have determined that 0.1 OD 600 is equivalent to 2.4 × 10 8 CFU/mL of T. denticola [15] or P. gingivalis [18,19], 1 × 10 8 CFU/mL of F. nucleatum [20] and to 6 × 10 7 CFU/mL of L. lactis [21].…”

Section: Bacteria and Biofilm Growthmentioning

confidence: 99%

Modulation of pathogenic oral biofilms towards health with nisin probiotic

Radaic

Parks

et al. 2020

Journal of Oral Microbiology

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Background: Oral dysbiosis is an imbalance in the oral microbiome and is associated with a variety of oral and systemic diseases, including periodontal disease, caries, and head and neck/oral cancer. Although antibiotics can be used to control this dysbiosis, they can lead to adverse side effects and superinfections. Thus, novel strategies have been proposed to address these shortcomings. One strategy is the use of probiotics as antimicrobial agents, since they are considered safe for humans and the environment. Specifically, the Grampositive Lactococcus lactis, a species present in the oral and gut microbiota, is able to produce nisin, which has been used worldwide for food preservation. Objective: The objective of this study was to test whether a nisin probiotic can promote a healthier oral microbiome in pathogen-spiked oral biofilms. Results: We found that L. lactis can prevent oral biofilm formation and disrupt 24-h and 48h pre-formed biofilms. Finally, we demonstrate that both treatments, a nisin-producing L. lactis probiotic and nisin can decrease the levels of pathogens in the biofilms and return the diversity levels back to control or 'healthy' levels. Conclusion: A nisin-producing probiotic, can be used to treat 'disease-altered' biofilms and promote healthier oral biofilms, which may be useful for improving patient oral health.

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Virulence of oral Fusobacterium nucleatum from humans and non-human primates in mice

Cited by 5 publications

References 33 publications

Periodontal profile and presence of periodontal pathogens in young African-Americans from Salvador, BA, Brazil

Periodontal profile and presence of periodontal pathogens in young African-Americans from Salvador, BA, Brazil

Nisin and Nisin Probiotic Disrupt Oral Pathogenic Biofilms and Restore Their Microbiome Composition towards Healthy Control Levels in a Peri-Implantitis Setting

Modulation of pathogenic oral biofilms towards health with nisin probiotic

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