Virulence of six capsular serotypes of <i>Porphyromonas gingivalis</i> in a mouse model

Laine, ML; Winkelhoff, AJ Van

doi:10.1111/j.1399-302x.1998.tb00714.x

Cited by 140 publications

(162 citation statements)

References 14 publications

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“…It is already well established that P. gingivalis strains exhibit different degrees of pathogenicity based on their ability to invade host cells, disseminate to various organ/tissue sites, and induce disease [77,[98][99][100]. This phenomenon also appears to occur in APOs, as indicated by experimental infection in pregnant rats [48].…”

Section: P Gingivalis Strain-specific Effects On Apomentioning

confidence: 94%

Porphyromonas gingivalisand adverse pregnancy outcome

Reyes

Phillips

Wolfe

et al. 2017

Journal of Oral Microbiology

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Porphyromonas gingivalis is a Gram-negative, anaerobic bacterium considered to be an important pathogen of periodontal disease that is also implicated in adverse pregnancy outcome (APO). Until recently, our understanding of the role of P. gingivalis in APO has been limited and sometimes contradictory. The purpose of this review is to provide an overview of past and current research on P. gingivalis that addresses some of the controversies concerning the role of this organism in the pathogenesis of APO.ARTICLE HISTORY

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Section: P Gingivalis Strain-specific Effects On Apomentioning

confidence: 94%

Porphyromonas gingivalisand adverse pregnancy outcome

Reyes

Phillips

Wolfe

et al. 2017

Journal of Oral Microbiology

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“…Strains W83 and ATCC 33277 were selected as the virulent and avirulent strains, respectively, since many previous reports have compared their virulence-associated activities and disease-promoting characteristics in vitro and in vivo (12,20,31). To determine the degree of divergence, we used an approach that determines and ranks gene variations between the two strains based on the shape of the signal ratio distribution (19), thus alleviating the empirical determination of a cutoff.…”

Section: Discussionmentioning

confidence: 99%

Comparative Whole-Genome Analysis of Virulent and Avirulent Strains ofPorphyromonas gingivalis

Chen¹,

Hosogi²,

Nishikawa³

et al. 2004

J Bacteriol

100

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We used Porphyromonas gingivalis gene microarrays to compare the total gene contents of the virulent strain W83 and the avirulent type strain, ATCC 33277. Signal ratios and scatter plots indicated that the chromosomes were very similar, with approximately 93% of the predicted genes in common, while at least 7% of them showed very low or no signals in ATCC 33277. Verification of the array results by PCR indicated that several of the disparate genes were either absent from or variant in ATCC 33277. Divergent features included already reported insertion sequences and ragB, as well as additional hypothetical and functionally assigned genes. Several of the latter were organized in a putative operon in W83 and encoded enzymes involved in capsular polysaccharide synthesis. Another cluster was associated with two paralogous regions of the chromosome with a low G؉C content, at 41%, compared to that of the whole genome, at 48%. These regions also contained conserved and species-specific hypothetical genes, transposons, insertion sequences, and integrases and were located adjacent to tRNA genes; thus, they had several characteristics of pathogenicity islands. While this global comparative analysis showed the close relationship between W83 and ATCC 33277, the clustering of genes that are present in W83 but divergent in or absent from ATCC 33277 is suggestive of chromosomal islands that may have been acquired by lateral gene transfer.The identification of virulent strains of pathogenic bacteria, and consequently their virulence genes, is a basic doctrine of the microbial pathogenesis field. Historically, identification has depended on phenotypic properties, biochemical activities, and immunological classifications. Increasingly, these tests have been replaced by genomic DNA-based analyses that can be successfully adapted to identify species, strains, and even mutants within strains. The availability of complete genome sequences for many bacterial pathogens has further increased the accuracy and specificity of such tests. A new addition to the existing repertoire of DNA analyses is comparative genome profiling using DNA microarrays, and this technology has been adapted to identify genes associated with pandemic strains of Vibrio cholerae (9) and to distinguish virulent strains of group A Streptococcus (30), Helicobacter pylori (4), and Salmonella species (6).Porphyromonas gingivalis is a gram-negative oral anaerobe associated with periodontal disease in adults. The organism is the most-studied oral pathogen, partly because it produces several virulence factors that can be isolated and studied biochemically (reviewed in reference 16) and partly because it is relatively easy to grow and manipulate genetically. According to animal models of disease, strains are classified as virulent and avirulent, and studies with bacterial strains and defined mutants have validated both the models and putative virulence factors (2, 3, 11). Strains of P. gingivalis have been differentiated by restriction fragment length polymorphism analysis of...

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“…Confirmando estos datos, Jotwani y Cutler demostraron que la cepa P. gingivalis 381 con fimbria es más eficaz que la cepa mutante P. gingivalis DPG3 carente de fimbria en invadir y penetrar células dendríticas, inducir la expresión de HLA-DR, CD83, CD80 y CD86 y la secreción de IL-6, IL-10, IL-12 y TNF-α en células dendríticas, y estimular la proliferación y secreción de IFN-γ en linfocitos T (23) . Sobre la base de la antigenicidad de la cápsula extracelular de P. gingivalis, se han descrito 6 serotipos capsulares (K) diferentes: K1-K6 (25,26) . Al analizar la respuesta de las células dendríticas humanas ante estos distintos serotipos K de P. gingivalis se demostró que existen diferencias cuantitativas en la síntesis de citoquinas; en particular, los serotipos K1 y K2 mostraron un mayor potencial inmuno-estimulador comparado con los otros serotipos bacterianos (25) .…”

Section: Introductionunclassified

“…Sobre la base de la antigenicidad de la cápsula extracelular de P. gingivalis, se han descrito 6 serotipos capsulares (K) diferentes: K1-K6 (25,26) . Al analizar la respuesta de las células dendríticas humanas ante estos distintos serotipos K de P. gingivalis se demostró que existen diferencias cuantitativas en la síntesis de citoquinas; en particular, los serotipos K1 y K2 mostraron un mayor potencial inmuno-estimulador comparado con los otros serotipos bacterianos (25) . La variable inmunogenicidad de los distintos serotipos K de P. gingivalis también ha sido demostrada en linfocitos TCD4 + , en los que indujeron una respuesta Th distinta.…”

Section: Introductionunclassified