Virulence of six heterogeneous-origin Newcastle disease virus isolates before and after sequential passages in domestic chickens

Kommers, Gláucia D.; King, Daniel J.; Seal, Bruce S.; Brown, Corrie C.

doi:10.1080/0307945021000070750

Cited by 52 publications

(34 citation statements)

References 35 publications

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“…Moreover, mild lymphocytic myocarditis and slight nonpurulent encephalitis were detected only in chickens, and these lesions were described previously. 19,20 Immunohistochemical NDV-NP-positive signals were detected in infected chickens at a late stage in the degenerated pancreatic acinar cells and degenerated renal tubules, at the middle of the experimental period in the myocardium, but not in the brain, indicating no or too low virus replication in the brain tissue to be detected by IHC in addition to the direct effect of NDV on the pancreatic acinar cells, renal epithelial cells, and myocardial fibers. This finding is consistent with a previous observation in SPF chickens infected with NDV isolates of different virulence.…”

Section: Discussionmentioning

confidence: 99%

“…The cerebellum had multifocal gliosis with Purkinje cell loss and epithelial necrosis in the comb as well as NDV-NP-positive signals in some organs, including brain. 20 Chickens are the most susceptible to NDV infection among poultry species, whereas ducks and geese are the least susceptible; 17 for this reason, wild birds are considered to be the natural reservoir of NDV. 43 Unfortunately, pathological studies of Newcastle disease in ducks are rare, so it is difficult to compare our results with previous data.…”

Section: Discussionmentioning

confidence: 99%

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Comparative Study on the Pathogenesis of the Generated 9a5b Newcastle Disease Virus Mutant Isolate Between Chickens and Waterfowl

et al. 2012

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Lentogenic Newcastle disease viruses (NDVs), circulating among waterfowl, have the potential to become highly pathogenic by replication in chickens. The pathological studies that compare NDV infections in chickens and waterfowl are rare. The virulent 9a5b mutant NDV isolate was generated by passaging the lentogenic Goose/Alaska/415/91 NDV isolate in chickens. The pathogenesis of the virulent 9a5b mutant isolate is unknown in both chickens and waterfowl. In this study, the virulent 9a5b mutant NDV isolate was inoculated intranasally in 32-day-old specific pathogen-free white Leghorn chickens and Japanese commercial ducks. Unlike ducks, which remained clinically normal throughout the study, chickens had depression, gasping, oral discharges, and greenish-white soft feces. Gross and histologic lesion patterns as well as viral replication supported the differing clinical outcome. Ducks had slight inflammation mainly in respiratory and digestive tracts, whereas slight nonpurulent encephalitis, necrotizing pancreatitis, tubulointerstitial nephritis, and mild inflammation in respiratory and digestive tracts were detected in chickens. In agreement, interferon-beta (IFN-b)-immunopositive signals were more intense in lung tissue of ducks than that of chickens, and NDV replications were detected intensively in chicken tissues. These results suggest that the 9a5b mutant NDV isolate is more virulent in chickens, and slight histological lesions were induced in ducks even with virulent NDVs.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Comparative Study on the Pathogenesis of the Generated 9a5b Newcastle Disease Virus Mutant Isolate Between Chickens and Waterfowl

et al. 2012

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“…Clinicopathologically, the phenotype of Long Bien was similar to other previously described virulent NDV strains belonging to genotypes II, V, VI, and VIIa. 7,[19][20][21]37 Long Bien strain caused severe disease, with birds succumbing rapidly after infection. By IHC, the viral tropism was mainly for lymphoid tissues, but viral protein was also very evident in the respiratory epithelium of trachea, lung, and air sacs and in the renal epithelium.…”

Section: Discussionmentioning

confidence: 99%

Clinicopathological Characterization in Poultry of Three Strains of Newcastle Disease Virus Isolated From Recent Outbreaks

et al. 2010

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Newcastle disease is a severe threat to the poultry industry and is caused by Newcastle disease virus, a member of the genus Avulavirus, family Paramyxoviridae. The virus is rapidly evolving, and several new genotypes have been discovered in the past few years. Characterization of these strains is important to evaluate field changes, anticipate new outbreaks, and develop adequate control measures. Three Newcastle disease isolates (APMV-1/duck/Vietnam, Long Bien/78/2002, APMV-1/chicken/ Australia/9809-19-1107/1998, and APMV-1/double-crested cormorant/USA, Nevada/19529-04/2005) from recent outbreaks were investigated via clinicopathological assessment, immunohistochemistry (IHC), in situ hybridization, virus isolation, and serology in experimentally infected 4-week-old chickens. Phylogenetic studies showed that Australia isolate belongs to class II genotype I, Long Bien to class II genotype VIId, and Nevada cormorant to class II genotype V. Even though all 3 viruses had a virulent fusion protein cleavage site and ICPI values greater than 1.5, they all differed in their ability to cause clinical signs, in their lesions, and in their viral distribution in body tissues. The Long Bien isolate showed the most severe clinicopathological picture and the most widespread viral distribution. The Australia and Nevada cormorant isolates had a milder pathological phenotype, with viral replication restricted to only a few organs. The variability in clinicopathological characteristics despite the similarity in ICPI suggests that full clinicopathological assessment is necessary to fully characterize new isolates and that there are differences in pathogenesis among viruses of different genotypes.

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“…One of the other great scourges of poultry, Newcastle disease virus, has also been studied not only in the context of disease within domestic fowl (Meulemans et al ., 2002;Nakamura et al ., 2004), but also in turkeys (Wehmann et al ., 2003), pigeons and doves (Aldous et al ., 2003(Aldous et al ., , 2004Barbezange & Jestin, 2003, 2005Terregino et al ., 2003), geese (Wan et al ., 2004), ducks (Aldous et al ., 2003), and other species, including sea birds (Lee et al ., 2004b) and parrots (Kommers et al ., 2003).…”

Section: Viruses Of Wild Birds and Minor Domestic Speciesmentioning

confidence: 99%

Coronaviruses in poultry and other birds

2005

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Virulence of six heterogeneous-origin Newcastle disease virus isolates before and after sequential passages in domestic chickens

Cited by 52 publications

References 35 publications

Comparative Study on the Pathogenesis of the Generated 9a5b Newcastle Disease Virus Mutant Isolate Between Chickens and Waterfowl

Comparative Study on the Pathogenesis of the Generated 9a5b Newcastle Disease Virus Mutant Isolate Between Chickens and Waterfowl

Clinicopathological Characterization in Poultry of Three Strains of Newcastle Disease Virus Isolated From Recent Outbreaks

Coronaviruses in poultry and other birds

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