Virulence of the plant-associated endophytic fungus &lt;i&gt;Lecanicillium muscarium &lt;/i&gt;to diamondback moth larvae

Kuchár, Michal; Glare, Travis R.; Hampton, J. G.; Dickie, Ian A.; Christey, M. C.

doi:10.30843/nzpp.2019.72.257

Cited by 12 publications

(8 citation statements)

References 20 publications

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“…Previous studies have shown variability in lepidopteran mortality after the ingestion of EPF-colonized plants. Some of these studies have reported high mortality values after the ingestion of colonized discs [ 25 , 31 , 32 ], but others have only shown a reduction in fitness [ 33 , 34 ]. In the current study, the fitness of S. littoralis larvae feeding on Metarhizium -colonized discs and adults emerging from pupae developing from surviving larvae was reduced in terms of food consumption and reproductive potential, respectively.…”

Section: Discussionmentioning

confidence: 99%

Delving into the Causes and Effects of Entomopathogenic Endophytic Metarhizium brunneum Foliar Application-Related Mortality in Spodoptera littoralis Larvae

Garrido‐Jurado

Montes-Moreno

Sanz-Barrionuevo

et al. 2020

Insects

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The aim of the current study was to delve into the causes of mortality of Spodoptera littoralis larvae feeding on Metarhizium-colonized plants in the absence of fungal outgrowth on the cadavers as previous studies reported and to elucidate the possible indirect effects of this fungus-colonized diet. The effect was evaluated in experiments conducted using leaf discs of colonized plants and in planta using fungus-colonized whole plants. The mortality rates of larvae fed on Metarhizium-colonized melon leaves were 45.0% and 87.5%, and the average survival times were 6.6 and 3.1 days in experiments performed with discs and in planta, respectively. Notably, these mortality levels were not associated with observed apoptosis mediated by caspases 1, 3-7 and 8; thus, further investigation into the possible immune system reaction of the insect after the ingestion of colonized plants is required. The leaf consumption of S. littoralis larvae fed on melon-colonized leaves was lower than that on control plants in the disc experiments but not in experiments conducted in planta. In this regard, in experiments performed in planta, plant damage increased larval mortality in both fungally challenged and control larvae. There was also a meaningful effect of exposure to Metarhizium-colonized melon leaf discs on S. littoralis fitness, with significant reductions in 39.0% and 22.0% in female fecundity and egg fertility, respectively, detected in females emerging from pupae developing from larvae surviving exposure to colonized plant discs; all larvae died in the in planta experiments. Hence, the present work presents new findings revealing the high potential of endophytic entomopathogenic fungi to improve the outcome of foliar applications against chewing insects in the short, mid- and long term, by the reduction of the reproductive potential of surviving adults and reveals new insights into the development of bioassays with whole plants for more detailed evaluation of the impact of these fungi as endophytes used for plant protection.

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Section: Discussionmentioning

confidence: 99%

Delving into the Causes and Effects of Entomopathogenic Endophytic Metarhizium brunneum Foliar Application-Related Mortality in Spodoptera littoralis Larvae

Garrido‐Jurado

Montes-Moreno

Sanz-Barrionuevo

et al. 2020

Insects

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“…Originally, A. muscarius ARSEF 5128 was isolated from a greenhouse whitefly in Littlehampton (UK) ( Hall, 1982 ), B. bassiana ARSEF 3097 from a boll weevil in the Rio Grande Valley (USA) ( Wright, 1996 ) and C. fumosorosea ARSEF 3682 from a mealy bug in a greenhouse in Apopka (USA) ( Vidal et al., 1998 ). All strains have been shown to colonize plants as an endophyte upon artificial inoculation in various crops, including sweet pepper ( Kuchár et al., 2019 ; Rondot and Reineke, 2019 ; Nicoletti and Becchimanzi, 2020 ; Doherty et al., 2021 ; Wilberts et al., 2022 ). The strains were acquired from the Agricultural Research Service Collection of Entomopathogenic Fungal Cultures (ARSEF; New York, USA), and were stored as agar plugs in glycerol at -80°C.…”

Section: Methodsmentioning

confidence: 99%

Fungal strain and crop cultivar affect growth of sweet pepper plants after root inoculation with entomopathogenic fungi

Wilberts,

Rojas-Preciado,

Jacquemyn

et al. 2023

Front. Plant Sci.

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As endophytes, entomopathogenic fungi can protect plants against biotic and abiotic stresses and at the same time promote plant growth and plant health. To date, most studies have investigated whether Beauveria bassiana can enhance plant growth and plant health, while only little is known about other entomopathogenic fungi. In this study, we evaluated whether root inoculation of the entomopathogenic fungi Akanthomyces muscarius ARSEF 5128, B. bassiana ARSEF 3097 and Cordyceps fumosorosea ARSEF 3682 can promote plant growth of sweet pepper (Capsicum annuum L.), and whether effects are cultivar-dependent. Plant height, stem diameter, number of leaves, canopy area, and plant weight were assessed four weeks following inoculation in two independent experiments using two cultivars of sweet pepper (cv. ‘IDS RZ F1’ and cv. ‘Maduro’). Results showed that the three entomopathogenic fungi were able to enhance plant growth, particularly canopy area and plant weight. Further, results showed that effects significantly depended on cultivar and fungal strain, with the strongest fungal effects obtained for cv. ‘IDS RZ F1’, especially when inoculated with C. fumosorosea. We conclude that inoculation of sweet pepper roots with entomopathogenic fungi can stimulate plant growth, but effects depend on fungal strain and crop cultivar.

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“…Our results showed that larvae and pupae had a prolonged developmental period, and both fecundity and survival rates were reduced. Previous studies have also reported the effectiveness of various EPFs, such as B. bassiana and M. anisopliae, in suppressing insect pests [14,52,[61][62][63][64].…”

Section: Discussionmentioning

confidence: 99%

Endophytic Colonization by Beauveria bassiana and Metarhizium anisopliae in Maize Plants Affects the Fitness of Spodoptera frugiperda (Lepidoptera: Noctuidae)

et al. 2023

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The fall armyworm, Spodoptera frugiperda (Noctuidae; Lepidoptera), is a serious threat to food security as it has the potential to feed on over 353 plant species. To control this insect pest, endophytic colonization of entomopathogenic fungi (EPF) in plants is being considered as a safer and more effective alternative. This study evaluated the efficacy of two EPFs, Beauveria bassiana and Metarhizium anisopliae, for endophytic colonization using foliar spray and seed treatment methods on maize plants, and their impact on the survival, development, and fecundity of S. frugiperda. Both EPF effectively colonized the maize plants with foliar spray and seed treatment methods, resulting in 72–80% and 50–60% colonization rates, respectively, 14 days after inoculation. The EPF negatively impacted the development and fecundity of S. frugiperda. Larvae feeding on EPF-inoculated leaves had slower development (21.21 d for M. anisopliae and 20.64 d for B. bassiana) than the control treatment (20.27 d). The fecundity rate was also significantly reduced to 260.0–290.1 eggs/female with both EPF applications compared with the control treatment (435.6 eggs/female). Age-stage-specific parameters showed lower fecundity, life expectancy, and survival of S. frugiperda when they fed on both EPF-inoculated leaves compared with untreated leaves. Furthermore, both EPFs had a significant effect on population parameters such as intrinsic (r = 0.127 d−1 for B. bassiana, and r = 0.125 d−1 for M. anisopliae) and finite rate (λ = 1.135 d−1 for B. bassiana, and λ = 1.1333 d−1 for M. anisopliae) of S. frugiperda compared with the control (r = 0.133 d−1 and λ = 1.146 d−1). These findings suggest that EPF can be effectively used for the endophytic colonization of maize plants to control S. frugiperda. Therefore, these EPFs should be integrated into pest management programs for this pest.

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Virulence of the plant-associated endophytic fungus <i>Lecanicillium muscarium </i>to diamondback moth larvae

Cited by 12 publications

References 20 publications

Delving into the Causes and Effects of Entomopathogenic Endophytic Metarhizium brunneum Foliar Application-Related Mortality in Spodoptera littoralis Larvae

Delving into the Causes and Effects of Entomopathogenic Endophytic Metarhizium brunneum Foliar Application-Related Mortality in Spodoptera littoralis Larvae

Fungal strain and crop cultivar affect growth of sweet pepper plants after root inoculation with entomopathogenic fungi

Endophytic Colonization by Beauveria bassiana and Metarhizium anisopliae in Maize Plants Affects the Fitness of Spodoptera frugiperda (Lepidoptera: Noctuidae)

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