Virulence Pattern Analysis of Three Listeria monocytogenes Lineage I Epidemic Strains with Distinct Outbreak Histories

Wagner, Martin; Slaghuis, Jörg; Göbel, Werner; Vázquez‐Boland, José A.; Rychli, Kathrin; Schmitz‐Esser, Stephan

doi:10.3390/microorganisms9081745

Cited by 2 publications

(1 citation statement)

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“…Almost all dairy isolates sequenced in this study contained ami except for several lineage I isolates from facilities #71 (n = 4) and #131 (n = 1), and one lineage II isolate from facility #122 (WRLP88; ST399/CC14) (Figure 3). When looking at L. monocytogenes isolates from different global outbreaks, there are examples of isolates that had intact ami genes, truncated ami genes, and an isolate without the gene (Wagner et al, 2021). Truncated ami was also identified in some ST14 and ST121 L. monocytogenes isolates considered to be potentially persistent from a rabbit meat processing plant and 23 publicly available L. monocytogenes genomes (Palma et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

Genomic characterization of Listeria monocytogenes recovered from dairy facilities in British Columbia, Canada from 2007 to 2017

Brown,

Bland,

McIntyre

et al. 2024

Front. Microbiol.

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Listeria monocytogenes is a foodborne pathogen of concern in dairy processing facilities, with the potential to cause human illness and trigger regulatory actions if found in the product. Monitoring for Listeria spp. through environmental sampling is recommended to prevent establishment of these microorganisms in dairy processing environments, thereby reducing the risk of product contamination. To inform on L. monocytogenes diversity and transmission, we analyzed genome sequences of L. monocytogenes strains (n = 88) obtained through the British Columbia Dairy Inspection Program. Strains were recovered from five different dairy processing facilities over a 10 year period (2007–2017). Analysis of whole genome sequences (WGS) grouped the isolates into nine sequence types and 11 cgMLST types (CT). The majority of isolates (93%) belonged to lineage II. Within each CT, single nucleotide polymorphism (SNP) differences ranged from 0 to 237 between isolates. A highly similar (0–16 SNPs) cluster of over 60 isolates, collected over 9 years within one facility (#71), was identified suggesting a possible persistent population. Analyses of genome content revealed a low frequency of genes associated with stress tolerance, with the exception of widely disseminated cadmium resistance genes cadA1 and cadA2. The distribution of virulence genes and mutations within internalin genes varied across the isolates and facilities. Further studies are needed to elucidate their phenotypic effect on pathogenicity and stress response. These findings demonstrate the diversity of L. monocytogenes isolates across dairy facilities in the same region. Findings also showed the utility of using WGS to discern potential persistence events within a single facility over time.

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Section: Discussionmentioning

confidence: 99%