Virulence Potential, Antimicrobial Susceptibility and Phylogenetic Analysis of Corynebacterium pseudotuberculosis Isolated from Caseous Lymphadenitis in Sheep and Goats in Duhok City, Iraq

Abdulrahman, Rezheen F.

doi:10.17582/journal.aavs/2021/9.6.919.925

Cited by 2 publications

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“…Different genes, such as 16s rRNA ( Çetinkaya et al , 2002 ) and rpoB genes ( Khamis et al , 2004 ; Abdulrahman et al ., 2020 ; Abdulrahman, 2021 ), have been used to generate a phylogenetic tree in C. pseudotuberculosis isolated from CLA cases in sheep and goats. According to the phylogenetic analysis of 16s rRNA gene sequences, out of 20 isolates of C. pseudotuberculosis , 16 isolates were genetically identical clones, which is not in line with what was found by ERIC fingerprinting analysis, where all strains were grouped into 11 genotypes (11 ERIC types), resulting in a significant level of genetic variation.…”

Section: Discussionmentioning

confidence: 99%

First study on molecular epidemiology of caseous lymphadenitis in slaughtered sheep and goats in Duhok Province, Iraq

Khanamir,

Issa,

Abdulrahman

2023

Open Vet J

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Background: Caseous lymphadenitis (CLA) is a chronic suppurative bacterial infection caused by Corynebacterium pseudotuberculosis (C. pseudotuberculosis) affecting superficial and internal lymph nodes and internal organs of small ruminants. Aims: Through the use of molecular methods, this study aimed to estimate the prevalence of caseous lymphadenitis and it’s contributing factors as well as the degree of genetic diversity and epidemiological relationships among C. pseudotuberculosis isolates from slaughtered sheep and goats in various districts of Duhok Province, Iraq. Methods: A total of 18836 carcasses (15775 sheep and 3061 goats) were inspected by veterinarians at slaughterhouses (Duhok-Sumel (2453 sheep+627 goats), Zakho (6000 sheep), Bardarash (1117 sheep+379 goats), Amedi (413 sheep+178 goats) and Akre (5792 sheep+1877 goats)) in Duhok Province for detection the prevalence rate of CLA using molecular techniques. Results: The prevalence of the disease was 0.94% and 1.93% in sheep and goats, respectively. Sheep in Duhok-Sumel and goats in Amedi were at the higher risk of infection than the animals in another location, with a prevalence rate of 4.31% and 6.18%, respectively. Sheep and goats of older age were more susceptible. Females were more susceptible than males in all districts except Duhok-Sumel where the reverse was true. ERIC-PCR analysis grouped the bacterial isolates into 11 different genotypes. Maximum Likelihood phylogenetic tree of partial sequences of the 16S rRNA gene sequences of C. pseudotuberculosis revealed no divergent sequences discovered in this study. Conclusion: A strict control program needs to be applied to reduce the entrance of the pathogen from the neighboring countries.

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Section: Discussionmentioning

confidence: 99%

First study on molecular epidemiology of caseous lymphadenitis in slaughtered sheep and goats in Duhok Province, Iraq

Khanamir,

Issa,

Abdulrahman

2023

Open Vet J

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The Etiological and Antimicrobial Susceptibility Profiles of the Bacteria Obtained from Ovine Caseous Lymphadenitis Cases in the Çankırı Region, Türkiye

Tarhane,

Büyük

2024

Life

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Sheep caseous lymphadenitis (CLA) causes significant economic losses in the livestock sector by causing a loss in the quantity and quality of animal products and a loss in the breeding value of animals. Although the primary agent in CLA’s etiology is Corynebacterium pseudotuberculosis, some other opportunistic microorganisms also play a role. Therefore, the control and treatment of CLA necessitates the identification of the relevant etiological agents. This study aimed to conduct an in vitro culture and molecular characterization (PCR analysis and 16S rRNA sequencing) of the bacteria involved in sheep CLA cases reported in the Çankırı province of Türkiye and determine the antibiotic susceptibility of the case isolates. In total, 82 (16.4%) of 500 sheep in five farms were diagnosed with CLA. Following the culture of the superficial abscesses samples, C. pseudotuberculosis was identified in 30 (36.59%) as a result of PCR, Pseudomonas spp. in 8 (9.76%), and Enterobacter cancerogenus in 1 (1.22%), as a result of 16S rRNA sequencing. These data revealed extensive heterogeneity among the Pseudomonas isolates, with hints of derivation from a common ancestry for some and phylogenetic similarity to isolates from Germany, Malaysia, and India. In contrast to the high susceptibility to cefoperazone and lincomycin, the high resistances of C. pseudotuberculosis and Pseudomonas spp. isolates to cephalothin, ceftiofur, cloxacillin, amoxicillin, and bacitracin were remarkable. Based on these findings, it was concluded that for an effective treatment and control of ovine CLA cases, there is a need to consider the possible involvement of opportunistic bacteria other than the primary causative agent, C. pseudotuberculosis. It also contributed to increasing the country-specific sequence data and establishing new taxa from a universal perspective.

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Virulence Potential, Antimicrobial Susceptibility and Phylogenetic Analysis of Corynebacterium pseudotuberculosis Isolated from Caseous Lymphadenitis in Sheep and Goats in Duhok City, Iraq

Abstract: C . pseudotuberculosis is a Gram-positive, pleomorphic, non-motile, non-capsulated, non-spore forming, facultative intracellular microorganism causing caseous lymphadenitis (CLA) in sheep and goats (

Cited by 2 publications

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First study on molecular epidemiology of caseous lymphadenitis in slaughtered sheep and goats in Duhok Province, Iraq

First study on molecular epidemiology of caseous lymphadenitis in slaughtered sheep and goats in Duhok Province, Iraq

The Etiological and Antimicrobial Susceptibility Profiles of the Bacteria Obtained from Ovine Caseous Lymphadenitis Cases in the Çankırı Region, Türkiye

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