2019
DOI: 10.1128/iai.00688-18
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Virulence Traits of a Serogroup C Meningococcus and Isogenic cssA Mutant, Defective in Surface-Exposed Sialic Acid, in a Murine Model of Meningitis

Abstract: In serogroup C Neisseria meningitidis, the cssA (siaA) gene codes for an UDP-N-acetylglucosamine 2-epimerase that catalyzes the conversion of UDP-N-acetyl-α-d-glucosamine into N-acetyl-d-mannosamine and UDP in the first step in sialic acid biosynthesis. This enzyme is required for the biosynthesis of the (α2→9)-linked polysialic acid capsule and for lipooligosaccharide (LOS) sialylation. In this study, we have used a reference serogroup C meningococcal strain and an isogenic cssA knockout mutant to investigate… Show more

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Cited by 10 publications
(5 citation statements)
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“…VVA6 also carried the N-acetylneuraminate cytidylyltransferase ( NeuA ), which involved the final step of adding cytidine 5′-monophosphate to activate Neu5Ac ( 47 ). Modifying Neu5Ac has been employed by various bacteria, including Neisseria meningitidis , Neisseria gonorrhoeae , Helicobacter pylori , and Salmonella enterica , in order to evade the host immune response and enhance survival in vivo ( 48 52 ). Surprisingly, VVA6 also harbored putative N-acetylgalactosaminyl-diphosphoundecaprenol glucuronosyltransferase responsible for teichuronic acid biosynthesis, which is usually found in cell wall component of Gram-positive ( Fig.…”
Section: Resultsmentioning
confidence: 99%
“…VVA6 also carried the N-acetylneuraminate cytidylyltransferase ( NeuA ), which involved the final step of adding cytidine 5′-monophosphate to activate Neu5Ac ( 47 ). Modifying Neu5Ac has been employed by various bacteria, including Neisseria meningitidis , Neisseria gonorrhoeae , Helicobacter pylori , and Salmonella enterica , in order to evade the host immune response and enhance survival in vivo ( 48 52 ). Surprisingly, VVA6 also harbored putative N-acetylgalactosaminyl-diphosphoundecaprenol glucuronosyltransferase responsible for teichuronic acid biosynthesis, which is usually found in cell wall component of Gram-positive ( Fig.…”
Section: Resultsmentioning
confidence: 99%
“…Therefore, dynein could be important to get to the cell body and near the nucleus but also to get to dendrites, depending on the type of neuron used, suggesting that the HrpA interaction with dynein could be fundamental for N. meningitidis spreading inside the neurons. Indeed, the marked neurotropism of this microorganism was confirmed in a mouse model of meningitis showing preferential localization of N. meningitidis in the corpus callosum [ 97 ]. Furthermore, the frequent neurological sequelae in patients who survive meningococcal meningitis are well known, including focal deficits, hearing loss, cognitive impairment and epilepsy [ 98 ].…”
Section: Discussionmentioning
confidence: 99%
“…Interestingly, feeding mice used in this viral–bacterial infection model with Lactobacillus paracasei CNCM I-1518 appeared to boost host defences against infection [ 191 ]. There is also a mouse model of meningococcal meningitis based on intracisternal infection of adult mice, which has been used to demonstrate the virulence of the L-glutamate transporter GltT protein [ 192 ] and of siaA [ 193 ]. Inhibition of matrix metalloproteinases has been shown to attenuate brain damage in this experimental meningitis model [ 194 ].…”
Section: Vertebrate Animal Modelsmentioning
confidence: 99%