Virulent strain associated outer membrane proteins of Borrelia burgdorferi.

Skare, Jon T.; Shang, Ellen S.; Foley, Denise; Blanco, David R.; Champion, Cheryl I.; Mirzabekov, Tajib A.; Sokolov, Yuri; Kagan, Bruce L.; Miller, James N.; Lovett, Michael

doi:10.1172/jci118295

Cited by 99 publications

(167 citation statements)

References 65 publications

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“…These findings are consistent with the possibility that acquired resistance may be generated by molecules other than OspA. We have recently identified sev-eral virulent associated outer membrane proteins that might be targets of protective immunity (33). Other investigators have obtained data that are in agreement with this observation (34,35).…”

Section: Discussionsupporting

confidence: 88%

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Acquired resistance to Borrelia burgdorferi infection in the rabbit. Comparison between outer surface protein A vaccine- and infection-derived immunity.

Foley¹,

Wang²,

Wu³

et al. 1997

J. Clin. Invest.

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Section: Discussionsupporting

confidence: 88%

“…Our view is that recently described outer membrane spanning (Oms) proteins, including porins (33), may prove to be attractive vaccine candidates that might be used alone or in combination with the OspA protein to provide a higher degree of protection against larger inocula and strains of differing OspA serotypes.…”

Section: Discussionmentioning

confidence: 99%

Acquired resistance to Borrelia burgdorferi infection in the rabbit. Comparison between outer surface protein A vaccine- and infection-derived immunity.

Foley¹,

Wang²,

Wu³

et al. 1997

J. Clin. Invest.

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“…A similar 0.6-nS pore-forming activity as the one found here was previously described together with 12.6-nS pores in outer membrane vesicles from B. burgdorferi B31 (53). This 0.6-nS pore-forming activity was afterward attributed to Oms28 (29).…”

Section: Discussionsupporting

confidence: 88%

Study of the Protein Complex, Pore Diameter, and Pore-forming Activity of the Borrelia burgdorferi P13 Porin

Bárcena-Uribarri

Thein

Barbot

et al. 2014

Journal of Biological Chemistry

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Background: B. burgdorferi P13 has unique characteristics compared with other porins. Results: P13 is a homo-oligomer with a 0.6 nS conductance in 1 M KCl and a substrate cut-off of 400 Da. Conclusion: P13 represents a general diffusion pathway for small solutes into Borrelia. Significance: Understanding the molecular transport through P13 may play a role in designing more efficient antibiotic treatments against Borrelia infections.

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“…By using these cytoplasmic membrane and OM detection tools, we have developed a method for isolation of the leptospiral OM in the form of membrane vesicles. Our method is a modification of techniques that use buffers that destabilize the spirochetal OM and facilitate its release in the form of OMVs (4,5,33,34,38). These OM isolation techniques take advantage of the structural lability of the spirochetal OM to produce OMVs that can be separated from protoplasmic-cylinder (PC)components by isopycnic sucrose gradient density ultracentrifugation.…”

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confidence: 99%

Characterization of the Leptospiral Outer Membrane and Description of Three Novel Leptospiral Membrane Proteins

2002

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The outer membrane (OM) of the mammalian pathogen Leptospira kirschneri was isolated in the form of membrane vesicles by alkaline plasmolysis and separated from the protoplasmic cylinder by sucrose density gradient ultracentrifugation. All four components of the alkaline plasmolysis buffer, including 1.0 M NaCl, 27% sucrose (wt/vol), 2 mM EDTA, and 10 mM Tris (pH 9), were required for efficient OM release, as judged by recovery of leptospiral lipopolysaccharide. Two populations of OM vesicles (OMVs) were recovered, with peak concentrations found in the sucrose gradient at densities of 1.16 and 1.18 g/ml. Transmission electron microscopy revealed that the more buoyant OMV population was smaller (<0.1 m in diameter) than the denser OMV population (0.2 to 0.3 m in diameter). The densities of both populations of OMVs were distinct from that of the protoplasmic-cylinder material, which was found in the sucrose gradient at a density of 1.20 g/ml. The OMV fractions were free of protoplasmic-cylinder material, as judged by immunoblotting with antibodies to the endoflagellar sheath protein, heat shock protein GroEL, and two novel cytoplasmic membrane proteins, lipoprotein LipL31 and transmembrane protein ImpL63. The protein components of the OMVs were characterized by one-and two-dimensional immunoblotting and found to include previously described OM proteins (OMPs), including the porin OmpL1; the lipoproteins LipL32, LipL36, and LipL41; and the peripheral membrane protein P31 LipL45 . A number of less well-characterized OMPs were also identified, including those with molecular masses of 16, 21, 21.5, 22, 31, 36, 44, 48, 90, and 116 kDa. The 48-kDa OMP was identified as a novel OM lipoprotein designated LipL48. The use of membrane-specific markers in OM isolation techniques facilitates an accurate description of the leptospiral OM and its components.Identification and characterization of outer membrane (OM) components are essential in the development of a molecular understanding of bacterial structure and function. Development of techniques for isolation of the OM from Leptospira species and other spirochetes has been difficult because of their unique architecture and a lack of sensitive markers for the cytoplasmic membrane. Like enteric gram-negative bacteria, spirochetes have both an OM and a cytoplasmic membrane, separated by a periplasmic space. However, spirochetal architecture differs significantly from that of gram-negative bacteria in that the peptidoglycan layer of spirochetes is associated with the cytoplasmic membrane rather than the OM (22,24). For this reason, techniques developed for isolation of the gram-negative OM from components of the underlying cell wall and cytoplasmic membrane are unlikely to be useful in spirochetes.Several approaches have been used in the isolation of the leptospiral OM, including sodium dodecyl sulfate (SDS) treatment of salt-altered cells (2,7,25,28), Sarkosyl (27), and Triton X-114 extraction and phase partitioning (20,45). On the basis of these approaches, three classes of lep...

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Virulent strain associated outer membrane proteins of Borrelia burgdorferi.

Cited by 99 publications

References 65 publications

Acquired resistance to Borrelia burgdorferi infection in the rabbit. Comparison between outer surface protein A vaccine- and infection-derived immunity.

Acquired resistance to Borrelia burgdorferi infection in the rabbit. Comparison between outer surface protein A vaccine- and infection-derived immunity.

Study of the Protein Complex, Pore Diameter, and Pore-forming Activity of the Borrelia burgdorferi P13 Porin

Characterization of the Leptospiral Outer Membrane and Description of Three Novel Leptospiral Membrane Proteins

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