Virulotyping of Shigella spp. isolated from pediatric patients in Tehran, Iran

Ranjbar, Reza; Bolandian, Masoumeh; Behzadi, Payam

doi:10.1556/030.64.2017.007

Cited by 15 publications

(11 citation statements)

References 24 publications

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“…In total, an 8.2% prevalence of Shigellosis was defined using cultures and 11.3% using PCR. The sensitivity of the PCR method is higher than those of the culture methods 15,16. However, our result was almost similar to studies conducted in Kerman, Iran and the North of Ethiopia, which had reported frequencies of 9% and 13.3%, respectively 17,18.…”

Section: Discussionsupporting

confidence: 86%

Multidrug-resistant Shigella infection in pediatric patients with diarrhea from central Iran

Abbasi

Abtahi

Belkum

et al. 2019

IDR

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Background: Shigella spp. are primary pathogens of diarrhea in children worldwide. Emergence of resistance to fluoroquinolones and third-generation cephalosporins is crucial in the management of pediatric shigellosis. We determined the prevalence and the antibiotic resistance patterns of Shigella species isolated from pediatric patients in central Iran. Materials and methods: Pediatric diarrhea samples (n=230) were cultured on MacConkey and XLD agar media and in GN broth. Genus-specific PCR for ipaH was also used for detection directly from fecal specimens. Antibiotic resistance and the frequency of ESBL and AmpC genes were determined. Results: Out of the 230 samples, 19 (8.2%) cases of Shigella spp. were identified using culture. Twenty-six samples were positive by PCR (11.3%), S. flexneri (4/19; 21%) and S. sonnei (15/19; 78.9%) being the most detected. The highest antibiotic resistance rates were found for cotrimoxazole (19/19; 100%), ampicillin (16/19; 84.2%), cefixime (13/19; 68.4%) and ceftriaxone (12/19; 63.1%). Ten cases showed phenotypic ESBL presence and all these strains were positive for bla TEM , bla CTX-M-1 , and bla CTX-M-15 . Three strains were AmpC positive, all of which harbored bla CMY-2 and two contained bla CIT . Of the 19 Shigella isolates 5 (26.3%), 2 (10.5%), and 1 (5.2%) were phenotypically resistant to nalidixic acid, ciprofloxacin, and norfloxacin, respectively. Class 1 integron was found in 18 (94.7%) isolates whereas class 2 integron was found in 19 (100%) strains. Conclusion: We found a considerable presence of Shigella species with elevated antibiotic resistance levels. In particular, the resistance to third-generation cephalosporins (ESBL) and ciprofloxacin must be taken seriously.

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Section: Discussionsupporting

confidence: 86%

Multidrug-resistant Shigella infection in pediatric patients with diarrhea from central Iran

Abbasi

Abtahi

Belkum

et al. 2019

IDR

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“…The use of molecular techniques like PCR is a good choice when the number of samples is low. But in the case of a large number of specimens, an advanced pangenomic diagnostic tool like the DNA microarray is fully recommended [ 28 – 32 ].…”

Section: Discussionmentioning

confidence: 99%

The prevalence of qnr genes of A, B, S in Escherichia coli strains isolated from three major hospitals in Tehran, Iran

Abbasi

Ranjbar

2018

CEJU

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IntroductionEscherichia coli (E.coli) as an opportunistic pathogen is a major cause of the hospital infections. The main goal of this research was to determine the frequency of quinolone resistance genes (qnr) among E.coli pathotypes isolated from patients with urinary tract infections (UTIs).Material and methodsUrine samples were obtained from patients with UTIs in three major hospitals of Mofid, Bu Ali, and Vali-Asr during the year of 2015 in Tehran, Iran. The antibiogram was done for isolated bacterial isolates using nalidixic acid, norfloxacin, gentamicin, streptomycin, and chloramphenicol. Then the plasmids of the bacterial samples were extracted. PCR was used to detect qnr genes. Finally, the PCR products were run on a 1% agarose gel electrophoresis and the results were analyzed by the program SPSS version 22.ResultsOverall, 100 E.coli strains were isolated from patients with UTIs. The highest resistance rate was against Streptomycin. The frequency of the genes of qnrA, qnrB and qnrS were 0%, 25% and 36%, respectively. Moreover, the presence of the both genes of qnrB and qnrS was recognized in 10% of isolated bacterial strains.ConclusionsOur results indicated increasing rates of quinolone resistant E.coli strains circulating in hospitals under the study. Dissemination of these strains harboring qnr determinants is of particular concern.

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“…Shigella also has the ability to carry multiple plasmids in relation to virulence [40]. The ial gene was the most frequent virulence gene among isolated bacterial strains followed by ipaH, set1B, and set1A; and S. flexneri possessed all (ial 66%, ipaH 59%, set1A 12%, and set1B 22%) [35].…”

Section: Discussionmentioning

confidence: 99%

Gastroenteritis in Japanese Females Caused by Shigella flexneri without Definite Infection Route: A Case Report and Mini-Review of Literature

Usuda¹,

Yamada²,

Takeshima³

et al. 2017

J Clin Case Rep

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In Japan, the incidence of Shigellosis is low at approximately 150 cases per year, with the majority of these being imported, mainly from Asia. Sporadic domestic occurrences are also reported, the majority of which absent epidemiological association. We report a case of gastroenteritis caused by Shigella flexneri in a 60-year-old Japanese female who recovered fully following treatment with levofloxacin. While we were unable to identify the route of infection, we suspected imported frozen marine products that may have been handled or processed in an unsafe manner. Shigellosis should be included in the differential diagnosis when presented with a patient exhibiting symptoms of gastrointestinal infection, even in the absence of a history of travel to an endemic area.

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Virulotyping of Shigella spp. isolated from pediatric patients in Tehran, Iran

Cited by 15 publications

References 24 publications

<p>Multidrug-resistant <em>Shigella</em> infection in pediatric patients with diarrhea from central Iran</p>

<p>Multidrug-resistant <em>Shigella</em> infection in pediatric patients with diarrhea from central Iran</p>

The prevalence of qnr genes of A, B, S in Escherichia coli strains isolated from three major hospitals in Tehran, Iran

Gastroenteritis in Japanese Females Caused by Shigella flexneri without Definite Infection Route: A Case Report and Mini-Review of Literature

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