Virus adhesion to archetypal fomites: A study with human adenovirus and human respiratory syncytial virus

Wang, Xunhao; Tarabara, Volodymyr V.

doi:10.1016/j.cej.2021.132085

Cited by 13 publications

(16 citation statements)

References 90 publications

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“…For example, recent molecular dynamics simulations have suggested that numerous stable direct contacts between nanoparticles and polymer brushes can occur, even when the brushes are extremely phobic to the nanoparticles . Wang and Tarabara used quartz crystal microbalance with dissipation analysis to show the importance of interfacial interactions to the adsorption of viruses to surfaces . Other theoretical models and simulations have provided insights into the penetration of biomolecules and colloids into zwitterionic polymer brush systems and the interactions between them. − Therefore, a fundamental investigation is needed on the partitioning and transport of viruses permeating into polymer brushes grafted to membranes and the nature of the interactions of the brushes with biomolecules or colloids.…”

Section: Resultsmentioning

confidence: 99%

Microporous Polyethersulfone Membranes Grafted with Zwitterionic Polymer Brushes Showing Microfiltration Permeance and Ultrafiltration Bacteriophage Removal

Qin

Ziemann

Bar‐Zeev

et al. 2023

ACS Appl. Mater. Interfaces

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Virus removal from water using microfiltration (MF) membranes is of great interest but remains challenging owing to the membranes' mean pore sizes typically being significantly larger than most viruses. We present microporous membranes grafted with polyzwitterionic brushes (N-dimethylammonium betaine) that combine bacteriophage removal in the range of ultrafiltration (UF) membranes with the permeance of MF membranes. Brush structures were grafted in two steps: free-radical polymerization followed by atom transfer radical polymerization (ATRP). Attenuated total reflection Fourier transform infrared (ATR−FTIR) and X-ray photoelectron (XPS) verified that grafting occurred at both sides of the membranes and that the grafting increased with increasing the zwitterion monomer concentration. The log reduction values (LRVs) of the pristine membrane increased from less than 0.5 LRV for T4 (∼100 nm) and NT1 (∼50 nm) bacteriophages to up to 4.5 LRV for the T4 and 3.1 LRV for the NT1 for the brush-grafted membranes with a permeance of about 1000 LMH/bar. The high permeance was attributed to a high-water fraction in the ultra-hydrophilic brush structure. The high measured LRVs of the brush-grafted membranes were attributed to enhanced bacteriophages exclusion from the membrane surface and entrapment of the ones that penetrated the pores due to the membranes' smaller mean pore-size and crosssection porosity than those of the pristine membrane, as seen by scanning electron microscopy (SEM) and measured using liquid− liquid porometry. Micro X-ray fluorescence (μ-XRF) spectrometry and nanoscale secondary ion mass spectrometry showed that 100 nm Si-coated gold nanospheres accumulated on the surface of the pristine membrane but not on the brush-coated membrane and that the nanospheres that penetrated the membranes were entrapped in the brush-grafted membrane but passed the pristine one. These results corroborate the LRVs obtained during filtration experiments and support the inference that the increased removal was due to a combined exclusion mechanism and entrapment. Overall, these microporous brush-grafted membranes show potential for use in advanced water treatment.

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Section: Resultsmentioning

confidence: 99%

Microporous Polyethersulfone Membranes Grafted with Zwitterionic Polymer Brushes Showing Microfiltration Permeance and Ultrafiltration Bacteriophage Removal

Qin

Ziemann

Bar‐Zeev

et al. 2023

ACS Appl. Mater. Interfaces

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“…Recent modelling of the adhesion of enveloped and nonenveloped viruses on a variety of architype fomites (silica, nylon, stainless steel and polypropylene) has shown that under conditions when a virus and the fomite have surface charge of the same polarity, adsorption is stronger at a higher ionic strength, where the repulsive electrostatic interactions are screened out [83]. Whereas, if the polarity of a virus and surface are opposite, cleaning solutions with a high ionic strength can be used to screen out attractive electrostatic interactions.…”

Section: Stability Of Coronaviruses On Porous Versus Non-porous Surfacesmentioning

confidence: 99%

“…It is further argued that the efficiency of virus attachment to fomites is correlated with interfacial free energy, where anti-adhesion surfaces should have a high positive value of the interfacial free energy of its interaction with a virus (where high positive values indicate hydrophilicity). Therefore, for a virus with known surface tension parameters, it may be possible to select surfaces with as high an interfacial free energy as is practical to reduce virus adhesion, and hence aid recovery [83].…”

Section: Stability Of Coronaviruses On Porous Versus Non-porous Surfacesmentioning

confidence: 99%

Porous surfaces: stability and recovery of coronaviruses

et al. 2021

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The role of indirect contact in the transmission of SARS-CoV-2 is not clear. SARS-CoV-2 persists on dry surfaces for hours to days; published studies have largely focused on hard surfaces with less research being conducted on different porous surfaces, such as textiles. Understanding the potential risks of indirect transmission of COVID-19 is useful for settings where there is close contact with textiles, including healthcare, manufacturing and retail environments. This article aims to review current research on porous surfaces in relation to their potential as fomites of coronaviruses compared to non-porous surfaces. Current methodologies for assessing the stability and recovery of coronaviruses from surfaces are also explored. Coronaviruses are often less stable on porous surfaces than non-porous surfaces, for example, SARS-CoV-2 persists for 0.5 h–5 days on paper and 3–21 days on plastic; however, stability is dependent on the type of surface. In particular, the surface properties of textiles differ widely depending on their construction, leading to variation in the stability of coronaviruses, with longer persistence on more hydrophobic materials such as polyester (1–3 days) compared to highly absorbent cotton (2 h–4 days). These findings should be considered where there is close contact with potentially contaminated textiles.

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“…4−7 Various strategies for the detection of viruses have been developed and achieved fruitful results. These include electrochemistry, 8,9 Raman spectroscopy, 10−12 quartz crystal microbalance (QCM), 13,14 the polymerase chain reaction (PCR), 15,16 the enzyme-linked immunosorbent assay, 17,18 resonance light scattering (RLS), 19 fluorescence spectroscopy, 20,21 and so on. Among them, real-time fluorescence quantitative PCR is a commonly employed method that has achieved good results in the rapid and quantitative detection of SARS-CoV-2.…”

mentioning

confidence: 99%

“…The history of human development is also the history of its fight against viruses, and countless people died in this endless struggle. − The rapid and highly sensitive detection of viruses is a key factor in reducing losses and ultimately winning the war against viruses, and this effort has drawn widespread attention from governments and scientists worldwide. − Various strategies for the detection of viruses have been developed and achieved fruitful results. These include electrochemistry, , Raman spectroscopy, − quartz crystal microbalance (QCM), , the polymerase chain reaction (PCR), , the enzyme-linked immunosorbent assay, , resonance light scattering (RLS), fluorescence spectroscopy, , and so on. Among them, real-time fluorescence quantitative PCR is a commonly employed method that has achieved good results in the rapid and quantitative detection of SARS-CoV-2. , The method has been used to successfully screen many positive samples, thereby making a major contribution to limiting the spread of the virus.…”

mentioning

confidence: 99%

Construction of an Ultrasensitive Molecularly Imprinted Virus Sensor Based on an “Explosive” Secondary Amplification Strategy for the Visual Detection of Viruses

et al. 2022

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Viral outbreaks have caused great disruptions to the economy and public health in recent years. The accurate detection of viruses is a key factor in controlling and overcoming epidemics. In this study, an ultrasensitive molecularly imprinted virus sensor was developed based on an “explosive” secondary amplification strategy. Magnetic particles coated with carbon quantum dots (Fe3O4@CDs) were used as carriers and fluorescent probes, while aptamers were introduced into the imprinting layer to enhance the specific recognition of the target virus enterovirus 71 (EV71). When EV71 was captured by the imprinted particles, the fluorescence of the CDs was quenched, especially after binding to the aptamer-modified ZIF-8 loaded with a large amount of phenolphthalein, thereby resulting in signal amplification. Then, when adjusting the pH of the solution to 12, the decomposition of ZIF-8 released phenolphthalein, which turned the solution red, leading to the second “explosive” amplification of the signal. Therefore, the detection of EV71 with ultrasensitivity was achieved, which allows for visual detection by the naked eye in the absence of any instruments. The detection limits for fluorescence and visualization detection were 8.33 fM and 2.08 pM, respectively. In addition, a satisfactory imprinting factor of 5.4 was achieved, and the detection time only needed 20 min. It is expected that this fluorescence-colorimetric dual-mode virus molecularly imprinted sensor will show excellent prospects in epidemic prevention and rapid clinical diagnosis.

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Virus adhesion to archetypal fomites: A study with human adenovirus and human respiratory syncytial virus

Cited by 13 publications

References 90 publications

Microporous Polyethersulfone Membranes Grafted with Zwitterionic Polymer Brushes Showing Microfiltration Permeance and Ultrafiltration Bacteriophage Removal

Microporous Polyethersulfone Membranes Grafted with Zwitterionic Polymer Brushes Showing Microfiltration Permeance and Ultrafiltration Bacteriophage Removal

Porous surfaces: stability and recovery of coronaviruses

Construction of an Ultrasensitive Molecularly Imprinted Virus Sensor Based on an “Explosive” Secondary Amplification Strategy for the Visual Detection of Viruses

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