Virus Excretion and Antibody Response in Saliva in Natural Mumps

Effect of age on the incidence of aseptic meningitis following immunization with monovalent mumps vaccine

“…After diagnosing aseptic meningitis, the cerebrospinal fluid was frozen and transferred to the clinical laboratory at the Kitasato Institutes on dry ice within 48 h after sampling. Mumps virus infection was confirmed by positive virus isolation in Vero cells and/or by the detection of the mumps virus genome through nested real time-PCR [8,9]. Nested PCR was performed targeting the part of genome regions of the phosphoprotein and/or hemagglutinin-neuraminidase gene [10][11][12][13][14].…”

“…Nested PCR was performed targeting the part of genome regions of the phosphoprotein and/or hemagglutinin-neuraminidase gene [10][11][12][13][14]. Genomic differentiation of the vaccine strain from wild strains was performed by a sequence analysis of the phosphoprotein and hemagglutinin-neuraminidase genes in all hospitalized meningitis cases [9][10][11]15,16]. We purified the PCR products and analyzed the DNA sequences bidirectionally with a Dye Terminator Sequencing Kit (Applied Biosystems Japan Inc., Tokyo, Japan) using an automated nucleotide analyzer (377A DNA Sequencer, Applied Biosystems, Foster City, CA, USA).…”

Guidance for Isolation Precautions for Mumps in the United States: A Review of the Scientific Basis for Policy Change

“…[3,26,[28][29][30][31][32][33][34][35][36][37][38][39][40] (Table 1). Of the 15 studies, 8 (7 conducted in the United States and Japan between 1934 and 1975 before availability of mumps vaccine) included only unvaccinated mumps case patients, 1 included vaccinated and unvaccinated case patients, and for 6 studies vaccination status of case patients was not reported [3,26,[28][29][30][31][32][33][34][35][36][37][38][39][40]. Four of the 15 studies summarized virus isolation from multiple saliva specimens collected prospectively after exposure, before and after onset of disease or infection [3,26,31,38] (Figure 1).…”

Characterization of Large Mumps Outbreak among Vaccinated Palestinian Refugees

“…Mumps virus (MuV)-specific immunoglobulin M (IgM) response usually precedes the IgG response early in the infection and wanes within the first 2 to 6 months (20). MuV is present in the saliva of infected individuals for several days before the onset of clinical disease and for up to 5 days afterwards (9,27). The virus can also be detected in urine for several weeks after the onset of mumps (33).…”