Virus-expressed, recombinant single-chain antibody blocks sporozoite infection of salivary glands in Plasmodium gallinaceum-infected Aedes aegypti.

Capurro, Margareth de Lara; Coleman, Judy; Beerntsen, Brenda T.; Myles, K.M.; Olson, Ken E.; Rocha, Eliana Maria Maurício da; Krettli, Antoniana U.; James, Anthony A.

doi:10.4269/ajtmh.2000.62.427

Cited by 125 publications

(62 citation statements)

References 28 publications

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“…gambiae adult peritrophic matrix protein 1 (AgAperl) promoter fused to PLA2 led to the accumulation of PLA2 in midgut epithelial cells before a blood meal and its release into the lumen upon blood ingestion greatly affecting oocyst formation (Abraham et al, 2005). Transient and stable transformation studies using single-chain antibodies (scFv) targeting important parasite ligands or other expression products have been shown to reduce intensities of infection of specific developmental stages (de Lara Capurro et al, 2000;Yoshida et al, 1999). Stable transgenics used mosquito vitellogenin-encoding (Vgl) and D. melanogaster ubiquitin gene promoters in parasite-infected Ae.…”

Section: Expression Of Antipathogen Effector Genes-a Major Applicatiomentioning

confidence: 99%

Gene Expression Studies in Mosquitoes

Chen¹,

Mathur²,

James³

2008

Advances in Genetics

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Research on gene expression in mosquitoes is motivated by both basic and applied interests. Studies of genes involved in hematophagy, reproduction, olfaction, and immune responses reveal an exquisite confluence of biological adaptations that result in these highly-successful life forms. The requirement of female mosquitoes for a bloodmeal for propagation has been exploited by a wide diversity of viral, protozoan and metazoan pathogens as part of their life cycles. Identifying genes involved in hostseeking, blood feeding and digestion, reproduction, insecticide resistance and susceptibility/ refractoriness to pathogen development is expected to provide the bases for the development of novel methods to control mosquito-borne diseases. Advances in mosquito transgenesis technologies, the availability of whole genome sequence information, mass sequencing and analyses of transcriptomes and RNAi techniques will assist development of these tools as well as deepen the understanding of the underlying genetic components for biological phenomena characteristic of these insect species.

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Section: Expression Of Antipathogen Effector Genes-a Major Applicatiomentioning

confidence: 99%

Gene Expression Studies in Mosquitoes

Chen¹,

Mathur²,

James³

2008

Advances in Genetics

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“…With disease vectors, the intended function of the beneficial gene is typically to disrupt the transmission of pathogens. The prospects for this type of intervention have been boosted by the germline transformation of several mosquito species (Coates et al 1998;Catteruccia et al 2000;Grossman et al 2001;Allen et al 2001) and the demonstration that transgenes can be introduced that reduce or abolish the ability to transmit malaria or dengue (Olson et al 1996;de Lara Capurro et al 2000;Ito et al 2002). If these advances are to be translated into control strategies, mechanisms for driving transgenes through field populations in a self-sustaining manner are essential, and this has been identified as a priority research area (Alphey et al 2002).…”

Section: Introductionmentioning

confidence: 99%

Use ofWolbachiato drive nuclear transgenes through insect populations

Sinkins

Godfray

2004

Proc. R. Soc. Lond. B

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Wolbachia is an inherited intracellular bacterium found in many insects of medical and economic importance. The ability of many strains to spread through populations using cytoplasmic incompatibility, involving sperm modification and rescue, provides a powerful mechanism for driving beneficial transgenes through insect populations, if such transgenes could be inserted into and expressed by Wolbachia. However, manipulating Wolbachia in this way has not yet been achieved. Here, we demonstrate theoretically an alternative mechanism whereby nuclear rather than cytoplasmic transgenes could be driven through populations, by linkage to a nuclear gene able to rescue modified sperm. The spread of a 'nuclear rescue construct' occurs as long as the Wolbachia show imperfect maternal transmission under natural conditions and/or imperfect rescue of modified sperm. The mechanism is most efficient when the target population is already infected with Wolbachia at high frequency, whether naturally or by the sequential release of Wolbachia-infected individuals and subsequently the nuclear rescue construct. The results provide a potentially powerful addition to the few insect transgene drive mechanisms that are available.

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“…A variety of genes conferring disease refractoriness have been identified in nature and engineered in the laboratory. For example, with respect to malaria, Ito et al (2002) engineered a gene that saturates the receptor sites that the malaria parasite requires to pass through the mosquito gut following ingestion; de Lara Capurro et al (2000) developed antibodies that kill malaria parasites; Riehle et al (2006) discovered genes that govern refractoriness in natural populations; and Corby-Harris et al (2010) activated a signaling pathway that dramatically reduces both parasite development and mosquito longevity. Expression of RNAs that induce RNA interference targeting dengue virus has also been shown to reduce dengue transmission (Franz et al 2006).…”

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confidence: 99%

Semele: A Killer-Male, Rescue-Female System for Suppression and Replacement of Insect Disease Vector Populations

et al. 2011

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Two strategies to control mosquito-borne diseases, such as malaria and dengue fever, are reducing mosquito population sizes or replacing populations with disease-refractory varieties. We propose a genetic system, Semele, which may be used for both. Semele consists of two components: a toxin expressed in transgenic males that either kills or renders infertile wild-type female recipients and an antidote expressed in females that protects them from the effects of the toxin. An all-male release results in population suppression because wild-type females that mate with transgenic males produce no offspring. A release that includes transgenic females results in gene drive since females carrying the allele are favored at high population frequencies. We use simple population genetic models to explore the utility of the Semele system. We find that Semele can spread under a wide range of conditions, all of which require a high introduction frequency. This feature is desirable since transgenic insects released accidentally are unlikely to persist, transgenic insects released intentionally can be spatially confined, and the element can be removed from a population through sustained release of wild-type insects. We examine potential barriers to Semele gene drive and suggest molecular tools that could be used to build the Semele system.

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Virus-expressed, recombinant single-chain antibody blocks sporozoite infection of salivary glands in Plasmodium gallinaceum-infected Aedes aegypti.

Cited by 125 publications

References 28 publications

Gene Expression Studies in Mosquitoes

Gene Expression Studies in Mosquitoes

Use ofWolbachiato drive nuclear transgenes through insect populations

Semele: A Killer-Male, Rescue-Female System for Suppression and Replacement of Insect Disease Vector Populations

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