Virus-host protein co-expression networks reveal temporal organization and strategies of viral infection

Aguirre, Jacobo; Guantes, Raúl

doi:10.1016/j.isci.2023.108475

Cited by 3 publications

(1 citation statement)

References 86 publications

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“…Moreover, it was shown that viruses may interact with host genes that are risk factors for AD, such as genes regulating the processing of APP (Readhead et al., 2018). In fact, some viruses such as HSV1, Epstein–Barr virus (EBV), and Vaccinia virus (VACV) have all been reported to feature co‐expression networks specific to virus‐host systems (Aguirre & Guantes, 2023; López‐Lastra, 2022). As a result, several host protein interactors are differentially expressed during viral infection (Karunakaran et al., 2022).…”

Section: Alzheimer's Disease and Viral Infectionmentioning

confidence: 99%

Do microbes play a role in Alzheimer's disease?

Williams,

Lang,

Nicolas

et al. 2024

Microbial Biotechnology

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Alzheimer's disease is a complex and progressive condition that affects essential neurological functions such as memory and reasoning. In the brain, neuronal loss, synaptic dysfunction, proteinopathy, neurofibrillary tangles, and neuroinflammation are the hallmarks of Alzheimer's disease pathophysiology. In addition, recent evidence has highlighted that microbes, whether commensal or pathogenic, also have the ability to interact with their host and to regulate its immune system, therefore participating in the exchanges that lead to peripheral inflammation and neuropathology. Because of this intimate relationship, bacteria, viruses, fungi, and protozoa have been implicated in the development of Alzheimer's disease. Here, we bring together current and most recent evidence of the role of microbes in Alzheimer's disease, raising burning questions that need to be addressed to guide therapeutic approaches and potential prophylactic strategies.

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Section: Alzheimer's Disease and Viral Infectionmentioning

confidence: 99%

Do microbes play a role in Alzheimer's disease?

Williams,

Lang,

Nicolas

et al. 2024

Microbial Biotechnology

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Adaptation of turnip mosaic virus toArabidopsis thalianainvolves rewiring of VPg–host proteome interactions

Carrasco,

Ambrós,

Gutiérrez

et al. 2024

Virus Evolution

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The outcome of a viral infection depends on a complex interplay between the host physiology and the virus, mediated through numerous protein-protein interactions. In a previous study, we used high-throughput yeast two-hybrid (HT-Y2H) to identify proteins in Arabidopsis thaliana that bind to the proteins encoded by the turnip mosaic virus (TuMV) genome. Furthermore, after experimental evolution of TuMV lineages in plants with mutations in defense-related or proviral genes, most mutations observed in the evolved viruses affected the VPg cistron. Among these mutations, D113G was a convergent mutation selected in many lineages across different plant genotypes, including cpr5-2 with constitutive expression of systemic acquired resistance. In contrast, mutation R118H specifically emerged in the jin1 mutant with affected jasmonate signaling. Using the HT-Y2H system, we analyzed the impact of these two mutations on VPg’s interaction with plant proteins. Interestingly, both mutations severely compromised the interaction of VPg with the translation initiation factor eIF(iso)4E, a crucial interactor for potyvirus infection. Moreover, mutation D113G, but not R118H, adversely affected the interaction with RHD1, a zinc-finger homeodomain transcription factor involved in regulating DNA demethylation. Our results suggest that RHD1 enhances plant tolerance to TuMV infection. We also discuss our findings in a broad virus evolution context.

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Adaptation of turnip mosaic virus toArabidopsis thalianainvolves rewiring of VPg - host proteome interactions

Carrasco,

Ambrós,

Gutiérrez

et al. 2024

Preprint

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The outcome of a viral infection depends on a complex interplay between the host physiology and the virus, mediated through numerous protein-protein interactions. In a previous study we used high-throughput yeast two-hybrid (HT-Y2H) to identify proteins inArabidopsis thalianathat bind to the proteins encoded by the turnip mosaic virus (TuMV) genome. Furthermore, after experimental evolution of TuMV lineages in plants with mutations in defense-related or proviral genes, most mutations observed in the evolved viruses affected the VPg cistron. Among these mutations, D113G was a convergent mutation selected in many lineages across different plant genotypes. In contrast, mutation R118H specifically emerged in thejin1mutant with affected jasmonate signaling. Using the HT-Y2H system, we analyzed the impact of these two mutations on VPg’s interaction with plant proteins. Interestingly, both mutations severely compromised the interaction of VPg with the translation initiation factor eIF(iso)4E, a crucial interactor for potyvirus infection. Moreover, mutation D113G, but not R118H, adversely affected the interaction with RHD1, a zinc-finger homeodomain transcription factor involved in regulating DNA demethylation. Our results suggest that RHD1 enhances plant tolerance to TuMV infection.

show abstract

Virus-host protein co-expression networks reveal temporal organization and strategies of viral infection

Cited by 3 publications

References 86 publications

Do microbes play a role in Alzheimer's disease?

Do microbes play a role in Alzheimer's disease?

Adaptation of turnip mosaic virus toArabidopsis thalianainvolves rewiring of VPg–host proteome interactions

Adaptation of turnip mosaic virus toArabidopsis thalianainvolves rewiring of VPg - host proteome interactions

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