Virus-Induced Gene Silencing in Olive Tree (Oleaceae)

Κουδούνας, Κωνσταντίνος; Thomopoulou, Margarita; Angeli, Elisavet; Tsitsekian, Dikran; Rigas, Stamatis; Hatzopoulos, Polydefkis

doi:10.1007/978-1-0716-0751-0_13

Cited by 9 publications

(6 citation statements)

References 49 publications

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“…IPP, isopentenyl diphosphate; DMAPP, Dimethylallyl diphosphate; Glu, glucose; OeGES1, geraniol synthase; OeISY, iridoid synthase; OeOMES, oleoside methyl ester synthase; OeSXS, secoxyloganin synthase; OeGLU, oleuropein β-glucosidase; OeEAME1/2, elenolic acid methylesterase 1 and 2. plasmid were mixed 1:1 with cells harboring the pTRV2 constructs in inoculation medium (10 mM 2-N-morpholinoethanesulfonic acid pH 5.6, 10 mM MgCl 2 , and 150 µM acetosyringone). Olive plantlets having at least one pair of fully expanded leaves were Agroinoculated by gently pricking the leaves at the abaxial side as described (Koudounas et al, 2020). This procedure was repeated every 2 weeks until the observation of a phenotype.…”

Section: Agrobacterium-mediated Vigsmentioning

confidence: 99%

“…In this study, we recruited the recently described Agrobacterium-mediated virus-induced gene silencing (VIGS) methodology (Koudounas et al, 2020) to transiently silence OeGLU in olive seedlings and validate whether OeGLU is the major oleuropein β-glucosidase in planta. Besides, downregulation of the OeGLU enzyme, transient silencing of OeGLU unexpectedly affected the biosynthesis of upstream and downstream secoiridoids suggesting the existence of a feedback regulatory loop in this pathway.…”

Section: Introductionmentioning

confidence: 99%

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Silencing of Oleuropein β-Glucosidase Abolishes the Biosynthetic Capacity of Secoiridoids in Olives

et al. 2021

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Specialized metabolism is an evolutionary answer that fortifies plants against a wide spectrum of (a) biotic challenges. A plethora of diversified compounds can be found in the plant kingdom and often constitute the basis of human pharmacopeia. Olive trees (Olea europaea) produce an unusual type of secoiridoids known as oleosides with promising pharmaceutical activities. Here, we transiently silenced oleuropein β-glucosidase (OeGLU), an enzyme engaged in the biosynthetic pathway of secoiridoids in the olive trees. Reduction of OeGLU transcripts resulted in the absence of both upstream and downstream secoiridoids in planta, revealing a regulatory loop mechanism that bypasses the flux of precursor compounds toward the branch of secoiridoid biosynthesis. Our findings highlight that OeGLU could serve as a molecular target to regulate the bioactive secoiridoids in olive oils.

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Section: Agrobacterium-mediated Vigsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Silencing of Oleuropein β-Glucosidase Abolishes the Biosynthetic Capacity of Secoiridoids in Olives

et al. 2021

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“…VIGS has been applied in diverse plants, including herbs and fruit trees ( Dommes et al, 2019 ) but few forest trees ( Cui and Wang, 2017 ; Dommes et al, 2019 ). VIGS technology based on tobacco rattle virus (TRV) was successfully applied in Populus euphratica , Populus canescens ( Shen et al, 2015 ), H. brasiliensis ( Li et al, 2021c ), and Olea europaea ( Koudounas et al, 2020 ). Since VIGS can rapidly reduce the expression of target genes, it facilitates molecular function research in plants, including forest trees.…”

Section: Advancements In Transgenic Technologies For Forest Treesmentioning

confidence: 99%

Advances and Perspectives of Transgenic Technology and Biotechnological Application in Forest Trees

et al. 2021

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Transgenic technology is increasingly used in forest-tree breeding to overcome the disadvantages of traditional breeding methods, such as a long breeding cycle, complex cultivation environment, and complicated procedures. By introducing exogenous DNA, genes tightly related or contributed to ideal traits—including insect, disease, and herbicide resistance—were transferred into diverse forest trees, and genetically modified (GM) trees including poplars were cultivated. It is beneficial to develop new varieties of GM trees of high quality and promote the genetic improvement of forests. However, the low transformation efficiency has hampered the cultivation of GM trees and the identification of the molecular genetic mechanism in forest trees compared to annual herbaceous plants such as Oryza sativa. In this study, we reviewed advances in transgenic technology of forest trees, including the principles, advantages and disadvantages of diverse genetic transformation methods, and their application for trait improvement. The review provides insight into the establishment and improvement of genetic transformation systems for forest tree species. Challenges and perspectives pertaining to the genetic transformation of forest trees are also discussed.

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“…Since then, the term ‘VIGS’ has meant any approach that uses recombinant viruses to inhibit endogenous gene expression [ 4 , 5 ]. This transcription suppression technique has been mainly applied in the field and on horticultural crops [ 1 ], and is now also being applied in forest trees [ 6 ], i.e., Populus euphratica , Populus canescens [ 7 ], Hevea brasiliensis [ 8 ], and Olea europaea [ 9 , 10 ]. VIGS is an RNA-mediated reverse genetics technique in eukaryotes that involves different classes of small RNAs with specialized roles in gene silencing [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Virus-Induced Gene Silencing (VIGS): A Powerful Tool for Crop Improvement and Its Advancement towards Epigenetics

Zulfiqar

Farooq

Zhao

et al. 2023

IJMS

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Virus-induced gene silencing (VIGS) is an RNA-mediated reverse genetics technology that has evolved into an indispensable approach for analyzing the function of genes. It downregulates endogenous genes by utilizing the posttranscriptional gene silencing (PTGS) machinery of plants to prevent systemic viral infections. Based on recent advances, VIGS can now be used as a high-throughput tool that induces heritable epigenetic modifications in plants through the viral genome by transiently knocking down targeted gene expression. As a result of the progression of DNA methylation induced by VIGS, new stable genotypes with desired traits are being developed in plants. In plants, RNA-directed DNA methylation (RdDM) is a mechanism where epigenetic modifiers are guided to target loci by small RNAs, which play a major role in the silencing of the target gene. In this review, we described the molecular mechanisms of DNA and RNA-based viral vectors and the knowledge obtained through altering the genes in the studied plants that are not usually accessible to transgenic techniques. We showed how VIGS-induced gene silencing can be used to characterize transgenerational gene function(s) and altered epigenetic marks, which can improve future plant breeding programs.

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Virus-Induced Gene Silencing in Olive Tree (Oleaceae)

Cited by 9 publications

References 49 publications

Silencing of Oleuropein β-Glucosidase Abolishes the Biosynthetic Capacity of Secoiridoids in Olives

Silencing of Oleuropein β-Glucosidase Abolishes the Biosynthetic Capacity of Secoiridoids in Olives

Advances and Perspectives of Transgenic Technology and Biotechnological Application in Forest Trees

Virus-Induced Gene Silencing (VIGS): A Powerful Tool for Crop Improvement and Its Advancement towards Epigenetics

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