Virus infection in<i>Ipomoea batatas</i>and a method for its elimination

Linden, A.J. Over De; Elliott, Ruth F.

doi:10.1080/00288233.1971.10421665

Cited by 16 publications

(1 citation statement)

References 2 publications

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“…In vitro plantlet regeneration of sweet potato (Ipomoea batatas L.) has been reported utilizing storage root tissue [11,21], leaf tissue [18], and meristem tip culture [1,9,13,17]. In those studies regeneration occurred only from primary explants, and except for the meristem cultures, quantitative data were not presented.…”

Section: Introductionmentioning

confidence: 99%

Root and shoot initiation by leaf, stem, and storage root explants of sweet potato

Carswell

Locy

1984

Plant Cell Tiss Organ Cult

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Explants from stem, leaf, and storage root tissue of sweet potato (lpomoea batatas L.) cv. Jewel, were placed on media conairdng 0.1, 1.0, and 10mg/INAA with 0.1, 1.0, or 10 mg/IBA in a factorial experiment. Some callus formed in every treatment, but the best callus growth was on media containing 1.0 mg/1NAA and 10 mg/1BA. Roots formed over a range of treatments but were most prolific on the medium containing 1.0 mg/1NAA and 0.1 mg/IBA. Some de novo formed roots subsequently produced shoot buds in culture. Shoot formation increased the longer the original explants remained in culture without subculture. Roots could be subcultured indefinitely on agar solidified medium, but shoot regeneration did not occur after two subcultures. Shoot formation was greatest when the roots were subcultured on medium containing 1.0 mg/NAA and 0.1 mg]lBA. The cultivar 'Caromex' followed the same regeneration pathway, but the number of shoots formed was considerably less. Regeneration in both 'Jewel' and 'Caromex' explants was enhanced by light.

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