Virus isolation and full-length genome sequencing of a representative canine distemper virus wild type strain of the South America 2 clade

Romanutti, Carina; Keller, Leticia; Torre, J.L. La; Panzera, Yanina; Fuques, Eddie; Pérez, Ruben; Calderon, Marina Beatriz Gallo

doi:10.1016/j.jviromet.2020.113857

Cited by 7 publications

(6 citation statements)

References 32 publications

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“…3c). A previous study suggested that all wild type strains of CDV clustered in a similar way to the groups obtained as in H gene analysis, which is normally used to identify geographically distinct CDV lineages (Romanutti et al 2020). We observed a similar grouping in this study.…”

Section: Whole Genome Sequencing Based Evolutionary Dynamicssupporting

confidence: 87%

A Comprehensive Molecular Survey of Viral Pathogens Associated with Canine Gastroenteritis During 2018-21

Dema

Tallapally

Ganji

et al. 2022

Preprint

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Viral pathogens account for major aetiology of canine gastroenteritis in India. However, till date, there was no structured comprehensive study reported on the viral aetiology of canine gastroenteritis in India. To elucidate this, 475 rectal swabs collected over a period of three years (2018-2021) from clinical canine gastroenteritis cases were screened by real-time PCR for the presence of six majorly known enteric viruses (Canine parvovirus -2 (CPV-2), canine distemper virus (CDV), canine adenovirus-2 (CAdV-2), canine corona virus (CCoV), canine astrovirus (CaAstV), canine rotavirus (CRV)). 71.6% of the samples were found to harbour at least one of the five viruses tested (CPV-2/2a/2b/2c, CDV, CAdV-2, CCoV and CaAstV), whereas, there was no evidence of CRV. The overall incidence rate for each virus was found highest at 64.8% for CPV-2/2a/2b/2c (2a: 21.1%, 2b: 77.4%, 2c: 1.5%), followed by 8% for CDV, 7.2% for CaAstV, 5.9% for CCoV and 4.6% for CAdV-2 with 16.8% incidence for coinfections ranging from two to four viruses in different combinations. Further, for the first time we report the whole genome sequences of CDV, CCoV and CaAstV Indian isolates from canine gastroenteritis cases. Phylogenetic analysis of whole genome revealed that CDV clustered with Asia-1 lineage, CaAstV clustered with group III that consists of China and Brazil isolates, and CCoV clustered with group II that consists of China and Taipei isolates. This study adds to our knowledge on the prevalence of these pathogens in the Indian subcontinent and highlights the need for relevant and routine vaccine prophylactic measures. It is alarming to note that 70.3% of animals vaccinated with DHPPIL were found positive for at least one virus. Hence, regular molecular analysis of the prevalent viruses is crucial to address vaccination failures.

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Section: Whole Genome Sequencing Based Evolutionary Dynamicssupporting

confidence: 87%

A Comprehensive Molecular Survey of Viral Pathogens Associated with Canine Gastroenteritis During 2018-21

Dema

Tallapally

Ganji

et al. 2022

Preprint

View full text Add to dashboard Cite

show abstract

“…3c). A previous study suggested that all wild type strains of CDV clustered in a similar way to the groups obtained as in H gene analysis, which is normally used to identify geographically distinct CDV lineages [57]. We observed a similar grouping in this study.…”

Section: Whole Genome Sequencing Based Evolutionary Dynamicssupporting

confidence: 87%

“…Worldwide, there were limited reports of whole-genome sequences of CDV, CCoV and CaAstV isolated from dogs [55][56][57]; and we found no reports from India, till date. Hence, the whole genome of the three viruses (CDV, CaAstV, CCoV) was sequenced, extracted and the ORF regions were predicted.…”

Section: Whole Genome Sequencing Based Evolutionary Dynamicsmentioning

confidence: 72%

A Comprehensive Molecular Survey of Viral Pathogens Associated with Canine Gastroenteritis

Dema¹,

Tallapally²,

Ganji³

et al. 2022

SSRN Journal

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“…Worldwide, there have been a limited number of reports of whole-genome sequences of CDV, CCoV, and CaAstV isolated from dogs [ 54 – 56 ], and we found no reports from India to date. Hence, the whole genome sequences of these three viruses were determined, and ORFs were predicted.…”

Section: Resultsmentioning

confidence: 75%

A comprehensive molecular survey of viral pathogens associated with canine gastroenteritis

et al. 2023

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Viral pathogens are the primary cause of canine gastroenteritis. However, few structured comprehensive studies on the viral etiology of canine gastroenteritis have been conducted. In this study, 475 rectal swabs collected over three years (2018-2021) from clinical canine gastroenteritis cases were screened for the presence of six major enteric viruses – canine parvovirus 2 (CPV-2), canine distemper virus (CDV), canine adenovirus 2 (CAdV-2), canine coronavirus (CCoV), canine astrovirus (CaAstV), and canine rotavirus (CRV) – by real-time PCR. The most frequently detected virus was CPV-2, which was present in 64.8% of the samples (subtype 2a, 21.1%; 2b, 77.4%; 2c, 1.5%), followed by CDV (8%), CaAstV (7.2%), CCoV (5.9%), and CAdV-2 (4.6%). Two to four of these viruses in different combinations were found in 16.8% of the samples, and CRV was not detected. The complete genome sequences of Indian isolates of CDV, CCoV, and CaAstV were determined for the first time, and phylogenetic analysis was performed. This study highlights the need for routine prophylactic vaccination with the appropriate vaccines. Notably, 70.3% of animals vaccinated with DHPPiL were found to be positive for at least one virus. Hence, regular molecular analysis of the prevalent viruses is crucial for addressing vaccination failures. Supplementary Information The online version contains supplementary material available at 10.1007/s00705-022-05674-6.

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Virus isolation and full-length genome sequencing of a representative canine distemper virus wild type strain of the South America 2 clade

Cited by 7 publications

References 32 publications

A Comprehensive Molecular Survey of Viral Pathogens Associated with Canine Gastroenteritis During 2018-21

A Comprehensive Molecular Survey of Viral Pathogens Associated with Canine Gastroenteritis During 2018-21

A Comprehensive Molecular Survey of Viral Pathogens Associated with Canine Gastroenteritis

A comprehensive molecular survey of viral pathogens associated with canine gastroenteritis

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