Virus-like particles in bovine turbinate cells infected with bovine virus diarrhoea/mucosal disease virus

Chasey, D.; Roeder, P. L.

doi:10.1007/bf01314835

Cited by 7 publications

(6 citation statements)

References 10 publications

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“…TEM-based studies from the times in which the genus Pestivirus was still belonging to the family Togaviridae reported that pestivirus-infected cells exhibited ultrastructural modifications of rER and contained small numbers of virus-like particles (VLPs) [51,52]. Gray and Nettleton (1987) reported that Border Disease Virus (BDV)-infected cells contained several profiles of ER and many dense lamellar bodies, which when transversely sectioned appeared as multiple rows of tubules, 33 nm in diameter [52].…”

Section: Architecture and Properties Of The Replication Factories mentioning

confidence: 99%

Membranous Replication Factories Induced by Plus-Strand RNA Viruses

Romero-Brey

Bartenschlager

2014

Viruses

236

242

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In this review, we summarize the current knowledge about the membranous replication factories of members of plus-strand (+) RNA viruses. We discuss primarily the architecture of these complex membrane rearrangements, because this topic emerged in the last few years as electron tomography has become more widely available. A general denominator is that two “morphotypes” of membrane alterations can be found that are exemplified by flaviviruses and hepaciviruses: membrane invaginations towards the lumen of the endoplasmatic reticulum (ER) and double membrane vesicles, representing extrusions also originating from the ER, respectively. We hypothesize that either morphotype might reflect common pathways and principles that are used by these viruses to form their membranous replication compartments.

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Section: Architecture and Properties Of The Replication Factories mentioning

confidence: 99%

Membranous Replication Factories Induced by Plus-Strand RNA Viruses

Romero-Brey

Bartenschlager

2014

Viruses

236

242

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“…Die Partikel von durchschnittlich 50 nm Durchmesser erscheinen als Cores mit allerdings wenig elektronendichtem Zentrum in der Zytoplasmamatrix, zuerst hauptsachlich der Golgi-Region, reifen durch Budding in intraplasmatische Zisternen hinein und sind spater zahlreich in Plasmavakuolen zu finden. Die reifen EA-Partikel differieren im Schnittbild von den fur BVD-und SP-Virus beschriebenen (4,13). Ein Budding an der Zellmembran haben die Autoren ebenfalls nicht nachweisen konqen.…”

Section: Ultrahistologiscbe Befunde Mit Dern Nichtcytopathogenen Stammunclassified

“…Ultrahistologische Studien an BVD-infizierten Kulturzellen unter ahnlicher Fragestellung liegen nur wenige vor (2,4,11). Sie wurden ausschliefllich an Modellen mit cytopathogenen BVD-Sthmen gewonnen.…”

Section: Introductionunclassified

Untersuchungen über die Vermehrung von cytopathogenem und nichtcytopathogenem BVD‐Kulturvirus II. Ultrahistologische Befunde

Mahnel

1984

Zentralblatt für Veterinärmedizin Reihe B

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Zusammenfassung Die Vermehrung von zwei cytopathogenen und einem nichtcytopathogenen BVD‐Stamm in Kulturzellen aus bovinen embryonalen Lungen wurde ultrahistologisch‐elektronenoptisch untersucht. In mit cytopathogenen Viren infizierten Zellen entstehen in Kernnähe, aber auch in anderen Plasmazonen, besonders jedoch in Bereichen mit vermehrtem rauhem endoplasmatischem Retikulum sowie damit umgebenen Zisternen, behüllte Viruspartikel mit elektronendichtem Core und durchschnittlich etwa 55 nm Größe. Eine Umhüllung an intraplasmatischen Membranen mittels Budding konnte nicht nachgewiesen werden. Wenige Stunden später überwiegen im Zytoplasma infizierter Zellen mit rauhem aber auch glattem endoplasmatischem Retikulum begrenzte kleinere bis sehr große Vakuolen, die einzelne reife Viruspartikel enthalten. Solche Zellen sind bereits weitgehend degeneriert und gehen in Lysis über. Mit nichtcytopathogenem Virus persistierend infizierte embryonale Rinderlungenzellen wiesen nur geringgradige Degenerationsmerkmale auf. Viruspartikel der oben beschriebenen Art wurden nur in sehr geringer Zahl im bereits behüllten Status in kleinen und größeren Vesikeln nachgewiesen, seltener in kernnahen kleinen Vakuolen. Die intraplasmatische Vakuolenbildung war in solchen Zellen wesentlich schwächer ausgeprägt und auf weniger Zellen beschränkt als bei cytopathogenen Virus‐Zellsystemen. Danksagung Frau Ursula Wolf danke ich für die qualifizierte technische Assistenz in Ultrahistologie und Elektronenmikroskopie. Summary Studies on the replication of cytopathic and noncytopathic BVD virus in cell culture II. Ultrahistological findings The replication of two cytopathic and one noncytopathic strains of BVD virus in cell cultures of bovine embryonic lung was investigated by ultrahistology. Enveloped virus particles with electron dense cores and an average size of 55 nm developing near the nucleus as well as in other regions of the cytoplasm, especially in regions with enriched rough endoplasmatic reticulum and in cisternae, were seen in cells infected with cytopathic strains. Formation of envelopes by budding through intraplasmatic membranes could not be found. Small and very large vacuoles limited by rough and smooth endoplasmatic reticulum and containing single mature virus particles predominated in the cytoplasm of infected cells some hours later. Such cells are degenerated and become lytic. Bovine embryonic lung cells persistently infected with noncytopathic virus strains showed only little degeneration. Isolated, enveloped virus particles as described above were found in small and large vesicles and only rarely in small vacuoles near the nucleus. The formation of intraplasmatic vacuoles was less distinct than in systems with cytopathic viruses and mostly limited to a few cells. Résumé Recherches sur la multiplication de la culture du virus cytopathogène et non cytopathogène II. Résultats ultrahistologiques La multiplication de deux souches BVD cytopathogènes et d'une souche BVD cytopathogène en culture de cellules de poumon embryonnaire bovin...

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“…Virus particles in electron micrographs appear as pleomorphic, mostly spherical, membrane-bound structures (Bielefeldt-Ohmann and Bloch, 1982). The viral core is an isometric, electron-dense structure, approximately 20 -30 nm in diameter (Horzinek et al, 1971; Chasey and Roeder, 1981;Gray and Nettleton, 1987). Surface projections cannot be distinguished on purified virus or virus particles in cells and tissues (Stott et al, 1974;Bielefeldt-Ohmann and Bloch, 1982).…”

Section: Physical-chemical Characteristicsmentioning

confidence: 99%

“…Viral replication takes place in the cell cytoplasm, closely associated with the endoplasmic reticulum. Budding of viral particles into cytoplasmic vesicles, endoplasmic reticulum modiHed into tubules (Chasey and Roeder, 1981;Gray and Nettleton, 1987), and autophagosomes or golgi networks (Bielefeldt-Ohmann et al, 1988) has been observed.…”

Section: Viral Growth Characteristicsmentioning

confidence: 99%

Extraction and epitopic analysis of the 53 kd envelope glycoprotein of bovine viral diarrhea virus

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Virus-like particles in bovine turbinate cells infected with bovine virus diarrhoea/mucosal disease virus

Cited by 7 publications

References 10 publications

Membranous Replication Factories Induced by Plus-Strand RNA Viruses

Membranous Replication Factories Induced by Plus-Strand RNA Viruses

Untersuchungen über die Vermehrung von cytopathogenem und nichtcytopathogenem BVD‐Kulturvirus II. Ultrahistologische Befunde

Extraction and epitopic analysis of the 53 kd envelope glycoprotein of bovine viral diarrhea virus

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