Virus-like particles nanoreactors: from catalysis towards bio-applications

Su, Yuqing; Liu, Beibei; Huang, Zhenkun; Teng, Zihao; Yang, Liulin; Zhu, Jie; Huo, Shuaidong; Liu, Aijie

doi:10.1039/d3tb01112g

Cited by 4 publications

(1 citation statement)

References 181 publications

(267 reference statements)

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“…So far, a number of different protein-based compartmentalization systems have been used for enzyme nanoreactor applications. These include ferritins 23 , viral capsids 24 , bacterial microcompartments (BMCs) 25 , lumazine synthase 26 , de novo designed protein shells 27 , and encapsulins 28 . The P22 viral capsid in particular has been used to create nanoreactors for alcohol oxidation 29 , decarboxylation 30 , hydrogen production 31 , and the degradation of endocrine disruptors 32 .…”

Section: Introductionmentioning

confidence: 99%

Pore engineering as a general strategy to improve protein-based enzyme nanoreactor performance

Kwon,

Andreas,

Giessen

2024

Preprint

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Enzyme nanoreactors are nanoscale compartments consisting of encapsulated enzymes and a selectively permeable barrier. Sequestration and co-localization of enzymes can increase catalytic activity, stability, and longevity, highly desirable features for many biotechnological and biomedical applications of enzyme catalysts. One promising strategy to construct enzyme nanoreactors is to repurpose protein nanocages found in nature. However, protein-based enzyme nanoreactors often exhibit decreased catalytic activity, partially caused by a mismatch of protein shell selectivity and the substrate requirements of encapsulated enzymes. No broadly applicable and modular protein-based nanoreactor platform is currently available. Here, we introduce a pore-engineered universal enzyme nanoreactor platform based on encapsulins - microbial self-assembling protein nanocompartments with programmable and selective enzyme packaging capabilities. We structurally characterize our protein shell designs via cryo-electron microscopy and highlight their polymorphic nature. Through fluorescence polarization assays, we show their improved molecular flux behavior and highlight their expanded substrate range via a number of proof-of-concept enzyme nanoreactor designs. This work lays the foundation for utilizing our encapsulin-based nanoreactor platform for future biotechnological and biomedical applications.

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Section: Introductionmentioning

confidence: 99%

Pore engineering as a general strategy to improve protein-based enzyme nanoreactor performance

Kwon,

Andreas,

Giessen

2024

Preprint

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Advancements in Functional Nanomaterials Inspired by Viral Particles

Sun,

Lian,

Tian

et al. 2024

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Virus‐like particles (VLPs) are nanostructures composed of one or more structural proteins, exhibiting stable and symmetrical structures. Their precise compositions and dimensions provide versatile opportunities for modifications, enhancing their functionality. Consequently, VLP‐based nanomaterials have gained widespread adoption across diverse domains. This review focuses on three key aspects: the mechanisms of viral capsid protein self‐assembly into VLPs, design methods for constructing multifunctional VLPs, and strategies for synthesizing multidimensional nanomaterials using VLPs. It provides a comprehensive overview of the advancements in virus‐inspired functional nanomaterials, encompassing VLP assembly, functionalization, and the synthesis of multidimensional nanomaterials. Additionally, this review explores future directions, opportunities, and challenges in the field of VLP‐based nanomaterials, aiming to shed light on potential advancements and prospects in this exciting area of research.

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Pore Engineering as a General Strategy to Improve Protein-Based Enzyme Nanoreactor Performance

Kwon,

Andreas,

Giessen

2024

ACS Nano

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Virus-like particles nanoreactors: from catalysis towards bio-applications

Abstract: Viral protein cages are self-assembled supramolecular structures that can be found in nature for compartmentalization. Exploiting their inherent properties, including precise nanoscale structures, monodispersity, and high stability, these architectures have...

Cited by 4 publications

References 181 publications

Pore engineering as a general strategy to improve protein-based enzyme nanoreactor performance

Pore engineering as a general strategy to improve protein-based enzyme nanoreactor performance

Advancements in Functional Nanomaterials Inspired by Viral Particles

Pore Engineering as a General Strategy to Improve Protein-Based Enzyme Nanoreactor Performance

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