2017
DOI: 10.1128/jvi.00362-17
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Virus-Like Vesicles of Kaposi's Sarcoma-Associated Herpesvirus Activate Lytic Replication by Triggering Differentiation Signaling

Abstract: Virus-like vesicles (VLVs) are membrane-enclosed vesicles that resemble native enveloped viruses in organization but lack the viral capsid and genome. During the productive infection of tumor-associated gammaherpesviruses, both virions and VLVs are produced and are released into the extracellular space. However, studies of gammaherpesvirus-associated VLVs have been largely restricted by the technical difficulty of separating VLVs from mature virions. Here we report a strategy of selectively isolating VLVs by u… Show more

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Cited by 20 publications
(19 citation statements)
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“…These light particles were able to initiate productive infection of axonally delivered PRV 180 as fast as UVPRV and did so in a PKA- and JNK-independent manner. Although herpesvirus infected cells yield large amounts of capsid-less virus-like particles (up to 81% in PRV [ 7 ], and 84% in EBV infection [ 8 ]) that contain viral envelope and tegument proteins and use exocytosis machinery similar to infectious virions [ 6 8 , 32 ], their function is not well understood. Recently, Gong et al, showed that such virus-like particles produced by Kaposi`s sarcoma associated virus (KSHV) induce B-cell differentiation signaling to promote lytic infection suggesting a role for light particles in productive versus latency switch [ 8 ].…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…These light particles were able to initiate productive infection of axonally delivered PRV 180 as fast as UVPRV and did so in a PKA- and JNK-independent manner. Although herpesvirus infected cells yield large amounts of capsid-less virus-like particles (up to 81% in PRV [ 7 ], and 84% in EBV infection [ 8 ]) that contain viral envelope and tegument proteins and use exocytosis machinery similar to infectious virions [ 6 8 , 32 ], their function is not well understood. Recently, Gong et al, showed that such virus-like particles produced by Kaposi`s sarcoma associated virus (KSHV) induce B-cell differentiation signaling to promote lytic infection suggesting a role for light particles in productive versus latency switch [ 8 ].…”
Section: Discussionmentioning
confidence: 99%
“…An important discovery came from complementation studies with light particle (LP) preparations generated after infection by a UL25 null PRV mutant that produced LP but no infectious virions. Herpesvirus infected cells typically produce light particles together with infectious virions [ 6 8 ]. LP contains no capsids or viral genomes, but carries outer tegument proteins and most of the viral envelope proteins.…”
Section: Introductionmentioning
confidence: 99%
“…To make EBV production medium, 25 ng μl −1 of tetradecanoyl phorbol acetate and 0.5 mM sodium butyrate are added to RPMI-1640 medium supplemented with 2% FBS. Tetradecanoyl phorbol acetate and sodium butyrate can both reactivate EBV from latency to virion-producing lytic replication, and the reduced level of FBS can minimize the secretion of EBV-like vesicles 39 . For each batch of EBV virion production, 30 T175 flasks of B95-8 cells at ~90% confluency were replenished with fresh EBV production medium.…”
Section: Methodsmentioning
confidence: 99%
“…VLVs have been described in both RNA and DNA viruses, including herpes simplex virus-1, hepatitis C virus and Kaposi's sarcoma-associated herpes virus. VLVs play functional roles during viral infections by facilitating communication among cells and enhancing viral infection [202][203][204] . Continued virus, and how best to normalize between samples and between sequencing runs-problems that are similar to transcriptome analysis of genetic isoforms.…”
Section: Box 1 | the Expanding Family Of Sub-viral Particlesmentioning
confidence: 99%