Virus PCR Assay Panels: An Alternative to the Mouse Antibody Production Test

Blank, Walter; Henderson, Keith; White, Lisa A.

doi:10.1038/laban0204-26

Cited by 17 publications

(11 citation statements)

References 21 publications

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“…Frequency of testing will depend on local circumstances. recent reports 25,32 suggest that the incidence of infectious agents in biological materials has markedly decreased over the past decades, but the risk still exists especially for those biologicals that have been stored for a long time and the source of which is ill-documented. For example, outbreaks of ectromelia in the USA caused by contaminated serum 33,34 underline the risk of agent transmission by biological materials.…”

Section: Every 3 Months Annuallymentioning

confidence: 99%

FELASA recommendations for the health monitoring of mouse, rat, hamster, guinea pig and rabbit colonies in breeding and experimental units

rabbits¹,

Mähler²,

et al. 2014

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The microbiological quality of experimental animals can critically influence animal welfare and the validity and reproducibility of research data. It is therefore important for breeding and experimental facilities to establish a laboratory animal health monitoring (HM) programme as an integrated part of any quality assurance system. FELASA has published recommendations for the HM of rodent and rabbit colonies in breeding and experimental units (Nicklas et al. Laboratory Animals, 2002), with the intention of harmonizing HM programmes. As stated in the preamble, these recommendations need to be adapted periodically to meet current developments in laboratory animal medicine. Accordingly, previous recommendations have been revised and shall be replaced by the present recommendations. These recommendations are aimed at all breeders and users of laboratory mice, rats, Syrian hamsters, guinea pigs and rabbits as well as diagnostic laboratories. They describe essential aspects of HM, such as the choice of agents, selection of animals and tissues for testing, frequency of sampling, commonly used test methods, interpretation of results and HM reporting. Compared with previous recommendations, more emphasis is put on the role of a person with sufficient understanding of the principles of HM, opportunistic agents, the use of sentinel animals (particularly under conditions of cage-level containment) and the interpretation and reporting of HM results. Relevant agents, testing frequencies and literature references are updated. Supplementary information on specific agents and the number of animals to be monitored and an example of a HM programme description is provided in the appendices.

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Section: Every 3 Months Annuallymentioning

confidence: 99%

FELASA recommendations for the health monitoring of mouse, rat, hamster, guinea pig and rabbit colonies in breeding and experimental units

rabbits¹,

Mähler²,

et al. 2014

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“…PCR assays appear to be most useful for the identification of parvoviruses in contaminated biomaterials (Bauer et al 2004;Blank et al 2004;Bootz et al 2003), target tissues from infected rodents (e.g., mesenteric lymph node and spleen) (Besselsen 1998;Besselsen et al 1995;Redig and Besselsen 2001;Wan et al 2006), and potentially infected feces and environmental surfaces Kunita et al 2006;Ueno et al 1997).…”

Section: Detectionmentioning

confidence: 99%

Lurking in the Shadows: Emerging Rodent Infectious Diseases

Besselsen¹,

Franklin²,

Livingston³

et al. 2008

ILAR Journal

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Rodent parvoviruses, Helicobacter spp., murine norovirus, and several other previously unknown infectious agents have emerged in laboratory rodents relatively recently. These agents have been discovered serendipitously or through active investigation of atypical serology results, cell culture contamination, unexpected histopathology, or previously unrecognized clinical disease syndromes. The potential research impact of these agents is not fully known. Infected rodents have demonstrated immunomodulation, tumor suppression, clinical disease (particularly in immunodeficient rodents), and histopathology. Perturbations of organismal and cellular physiology also likely occur. These agents posed unique challenges to laboratory animal resource programs once discovered; it was necessary to develop specific diagnostic assays and an understanding of their epidemiology and transmission routes before attempting eradication, and then evaluate eradication methods for efficacy. Even then management approaches varied significantly, from apathy to total exclusion, and such inconsistency has hindered the sharing and transfer of rodents among institutions, particularly for genetically modified rodent models that may not be readily available. As additional infectious agents are discovered in laboratory rodents in coming years, much of what researchers have learned from experiences with the recently identified pathogens will be applicable. This article provides an overview of the discovery, detection, and research impact of infectious agents recently identified in laboratory rodents. We also discuss emerging syndromes for which there is a suspected infectious etiology, and the unique challenges of managing newly emerging infectious agents.

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“…Recent experiences have shown that even cell-free biologicals have the potential to introduce agents to rodent colonies when imported (Lipman et al, 2000). However, PCR technology has supplanted mouse antibody production/RAP assays and allows direct testing of the materials themselves for the presence of infectious agents (Bauer et al, 2004;Bootz and Sieber, 2002;Blank et al, 2004). However, PCR technology has supplanted mouse antibody production/RAP assays and allows direct testing of the materials themselves for the presence of infectious agents (Bauer et al, 2004;Bootz and Sieber, 2002;Blank et al, 2004).…”

Section: Screening Of Imported Biological Materialsmentioning

confidence: 99%