Virus Resistance in Rabbit Kidney Cell Cultures Contaminated by a Protozoan Resembling Encephalitozoon cuniculi

Armstrong, John A.; Yh, Ke; Breinig, Mary C.; Ople, L.

doi:10.3181/00379727-142-37209

Cited by 11 publications

(1 citation statement)

References 2 publications

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“…In the present study, we explored the effect of experimental encephalitozoonosis on murine NK cell activity in vitro. Two pieces of evidence led us to believe that encephalitozoonosis might stimulate NK cell activity: (i) mice infected with E. cuniculi demonstrate increased resistance to transplanted tumors (1); and (ii) tissue-cultured rabbit kidney cells elaborate an interferon-like molecule after in vitro infection with E. cuniculi (2).…”

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confidence: 99%

Enhanced natural killer cell activity in experimental murine encephalitozoonosis

Niederkorn¹,

Brieland²,

Mayhew³

1983

Infect Immun

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Spleen cells from mice infected with the protozoan parasite Encephalitozoon cuniculi demonstrated enhanced in vitro cytolysis of YAC-1 lymphoma cells. Selective cell depletion experiments showed that the dominant cell population mediating cytolysis of YAC-1 tumor cells expressed the characteristic phenotype of murine natural killer (NK) cells because (i) pretreatment of spleen cells with anti-asialo GM 1 antiserum plus complement abolished the cytotoxic activity; (ii) augmented cytolysis was found in athymic nude mice; (iii) pretreatment of spleen cells with anti-Thy 1.2 plus complement did not affect the level of cytolysis; and (iv) nylon wool removal of adherent cells did not reduce the augmented cytolysis. The augmented cytolysis peaked 7 days after infection, gradually diminished, and finally returned to control levels by 21 days postinfection. The parasite-induced augmentation of NK cell activity was dose-dependent: inoculation of 107 parasites gave maximum enhancement, whereas 105 or 104 parasites had an insignificant

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confidence: 99%