Virus Structure

Samson, A. C. R.

doi:10.1007/978-1-4613-1759-3_3

Cited by 7 publications

(5 citation statements)

References 95 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The virus possesses a nonsegmented negative-strand RNA genome of 15 186 nucleotides (Phillips et al, 1998) which encodes six main structural proteins (Samson, 1988): nucleocapsid protein (NP), phosphoprotein (P), matrix protein (M), fusion protein (F), haemagglutinin-neuraminidase (HN) and large protein (L). The NP protein is the most abundant protein in the virus particles and together with genomic RNA makes up the core helical nucleocapsid structure of NDV.…”

Section: Newcastle Disease Virus (Ndv) Is a Member Of The Genusmentioning

confidence: 99%

Newcastle disease virus nucleocapsid protein: self-assembly and length-determination domains

Kho

Tan

Tey

et al. 2003

Journal of General Virology

View full text Add to dashboard Cite

The nucleocapsid protein (NP) of Newcastle disease virus expressed in E. coli assembled as ring-and herringbone-like particles. In order to identify the contiguous NP sequence essential for assembly of these particles, 11 N-or C-terminally deleted NP mutants were constructed and their ability to self-assemble was tested. The results indicate that a large part of the NP N-terminal end, encompassing amino acids 1 to 375, is required for proper folding to form a herringbone-like structure. In contrast, the C-terminal end covering amino acids 376 to 489 was dispensable for the formation of herringbone-like particles. A region located between amino acids 375 to 439 may play a role in regulating the length of the herringbone-like particles. Mutants with amino acid deletions further from the C-terminal end (84, 98, 109 and 114 amino acids) tended to form longer particles compared to mutants with shorter deletions (25 and 49 amino acids). Newcastle disease virus (NDV) is a member of the genusRubulavirus of the family Paramyxoviridae (Rima et al., 1995) and causes the highly contagious Newcastle disease (ND) in many avian species. The virus possesses a nonsegmented negative-strand RNA genome of 15 186 nucleotides (Phillips et al., 1998) which encodes six main structural proteins (Samson, 1988): nucleocapsid protein (NP), phosphoprotein (P), matrix protein (M), fusion protein (F), haemagglutinin-neuraminidase (HN) and large protein (L). The NP protein is the most abundant protein in the virus particles and together with genomic RNA makes up the core helical nucleocapsid structure of NDV. The genomic RNA is associated with the NP, P and L proteins to form the ribonucleoprotein complex (RNP), which serves as a template for RNA synthesis (Yusoff & Tan, 2001).The NP protein of paramyxoviruses has been the focus of many studies for years due to its crucial functional role during replication of the genomic RNA (Horikami et al., 1992; Curran et al., 1993;Myers et al., 1999). Much structural and functional information on this protein has been derived, mainly from other paramyxoviruses such as Sendai, measles and human parainfluenza type 2 (hPIV-2) viruses (Spehner et al., 1991;Buchholz et al., 1993Buchholz et al., , 1994Bankamp et al., 1996;Nishio et al., 1999). Like many other paramyxoviruses, the nucleocapsid of NDV possesses a herringbone-like structure (Alexander, 1988). Recombinant NP protein of Newcastle disease virus expressed in E. coli (Kho et al., 2001a) or the baculovirus expression system (Errington & Emmerson, 1997) is able to self-assemble to form ring-and herringbone-like structures which morphologically resemble the authentic nucleocapsid structures. Insertion of a 29 residue peptide containing the Myc epitope and His-tag sequences onto the C terminus of the NP protein inhibits assembly of the herringbone-like structures but does not impair formation of the ring-like particles (Kho et al., 2001a). Furthermore, this foreign peptide was shown to be exposed on the surface of the ring-like particle (Kho et al., 2001a;...

show abstract

Section: Newcastle Disease Virus (Ndv) Is a Member Of The Genusmentioning

confidence: 99%

Newcastle disease virus nucleocapsid protein: self-assembly and length-determination domains

Kho

Tan

Tey

et al. 2003

Journal of General Virology

View full text Add to dashboard Cite

show abstract

“…The genome of NDV has a single-stranded, negative-sense RNA genome of 15,200 nucleotides that contain six genes, which encode seven proteins. The matrix (M gene), the fusion (F gene), the hemagglutinin-neuraminidase (HN gene) and the RNA-dependent RNA polymerase (L gene) proteins, the nucleoprotein (NP gene), the phosphoprotein (P gene), and the V protein resulting from mRNA editing of the P gene [ 5 , 6 , 7 ].…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization of full fusion protein (F) of Newcastle disease virus genotype VIId isolated from Egypt during 2012-2016

Selim¹,

Selim²,

Arafa³

et al. 2018

Vet World

View full text Add to dashboard Cite

Aim:The aim of this work was to study the full F gene sequence of Newcastle disease virus (NDV) in regard to pathotyping and genotyping and to study the evolution of this NDV in Egypt.Materials and Methods:The present study was conducted using samples from seven suspected NDV flocks of vaccinated chickens during 2012-2016 from six governorates in Egypt. The NDV was successfully isolated from pathological specimens through inoculation in specific pathogen-free embryonated chicken eggs.Results:Pathogenicity of the NDV isolates has been estimated through intracerebral pathogenicity index and ranged from 1.66 to 1.73 which indicates the velogenic type of NDV isolates. Pathotyping and genotyping of these isolates were done through sequencing of full-length F gene. Results indicated that the seven NDV isolates showed characteristic cleavage site motif (112RRQKRF117) for the velogenic strains of NDV. Phylogenetic analysis of the F gene clustered these isolates within Group I of genotype VIId within Israeli strains NDV/IS/2015, NDV-Ch/SD883, and most of the Middle East strains. Six of seven sequenced isolates have six potential N-linked glycosylation sites. The neutralization epitope on the five antigenic sites of fusion is conserved in all Egyptian strains of this study except NDV-KFR-B7-2012 which has a substitution at D 170 N in epitope A4. In all our strains, 10 cysteine residues are recorded, except one loss of cysteine at residue 370 in both NDV-EG-35-2014 and NDV-GHB-328F-2016.Conclusion:All viruses in this study have 52 amino acid substitutions within fusion gene in compared with Lasota strain that reveals importance for its antigenic and structural function. The present work highlights the important need to sequence F gene of NDV genotype VIId to investigate the evolution of this NDV in Egypt.

show abstract

“…Newcastle disease has caused severe economic loss in the poultry industry worldwide due to the high costs of vaccinations and diagnostic laboratory investigations [10, 11]. The causative agent, Newcastle disease virus (NDV), is a single-strand, unsegmented negative-sense RNA virus comprised of six proteins: a large protein (L), hemagglutinin–neuraminidase (HN) protein, fusion protein (F), matrix protein (M), phosphoprotein (P), and nucleocapsid protein (NP) [12]. To date, the haemagglutination inhibition (HI) test is still the most widely used serological method for measuring anti-NDV antibody levels in poultry sera [13].…”

Section: Introductionmentioning

confidence: 99%

Nanobody-horseradish peroxidase fusion protein as an ultrasensitive probe to detect antibodies against Newcastle disease virus in the immunoassay

Sheng

Wang

et al. 2019

J Nanobiotechnol

View full text Add to dashboard Cite

Background Sensitive and specific antibodies can be used as essential probes to develop competitive enzyme-linked immunosorbent assay (cELISA). However, traditional antibodies are difficult to produce, only available in limited quantities, and ineffective as enzymatic labels. Nanobodies, which are single-domain antibodies (sdAbs), offer an alternative, more promising tool to circumvent these limitations. In the present work, a cELISA using nanobody-horseradish peroxidase (HRP) fusion protein firstly designed as a probe was developed for detecting anti-Newcastle disease virus (NDV) antibodies in chicken sera. Results In the study, a platform for the rapid and simple production of nanobody-HRP fusion protein was constructed. First, a total of 9 anti-NDV-NP protein nanobodies were screened from a immunised Bactrian camel. Then, the Nb5-HRP fusions were produced with the platform and used for the first time as sensitive reagents for developing cELISA to detect anti-NDV antibodies. The cut-off value of the cELISA was 18%, and the sensitivity and specificity were respectively 100% and 98.6%. The HI test and commercial ELISA kit (IDEXX) separately agreed 97.83% and 98.1% with cELISA when testing clinical chicken sera and both agreed 100% when testing egg yolks. However, for detecting anti-NDV antibodies in the sequential sera from the challenged chickens, cELISA demonstrated to be more sensitive than the HI test and commercial ELISA kit. Moreover, a close correlation (R 2 = 0.914) was found between the percent competitive inhibition values of cELISA and HI titers. Conclusions A platform was successfully designed to easily and rapidly produce the nanobody-HRP fusion protein, which was the first time to be used as reagents for establishing cELISA. Results suggest that the platform supports the development of a cELISA with high sensitivity, simplicity, and rapid detection of anti-NDV antibodies. Overall, we believe that the platform based on nanobody-HRP fusions can be widely used for future investigations and treatment other diseases and viruses. Electronic supplementary material The online version of this article (10.1186/s12951-019-0468-0) contains supplementary material, which is available to authorized users.

show abstract

Virus Structure

Cited by 7 publications

References 95 publications

Newcastle disease virus nucleocapsid protein: self-assembly and length-determination domains

Newcastle disease virus nucleocapsid protein: self-assembly and length-determination domains

Molecular characterization of full fusion protein (F) of Newcastle disease virus genotype VIId isolated from Egypt during 2012-2016

Nanobody-horseradish peroxidase fusion protein as an ultrasensitive probe to detect antibodies against Newcastle disease virus in the immunoassay

Contact Info

Product

Resources

About