Viruses in the Phytoreovirus Genus of the Reoviridae Family have the Same Conserved Terminal Sequences

Conserved terminal sequences of rice ragged stunt virus genomic RNA

“…The labelled dsRNA genome segments were separated by 10% PAGE (Laemmli, 1970). Individual genome segments were localized by autoradiography and eluted from the crushed gel as described by Kudo et al (1991). The separated labelled genome segments were precipitated by the addition of carrier tRNA and 2-5 volumes of ethanol.…”

“…Two types of conserved tetranucleotide sequence were found to be present at the Asamizu et al (1985). 1" Data taken from Kudo et al (1991). Data taken from Azuhata et aL (1990).…”

“…The 5'-and 3'-terminal nucleotide sequences of the genome segments of wound tumour virus (WTV), rice dwarf virus (RDV), rice gall dwarf virus (RGDV), all members of the Phytoreovirus genus, have been determined (Asamizu et al, 1985;Kudo et al, 1991). A conserved 5'-terminal tetranucleotide sequence [5' GG(U/C)A---] and Y-terminal tetranucleotide sequence [---(U/C)GAU 3'] are shared by all genome segments of viruses in the genus Phytoreovirus.…”

“…Data taken from Azuhata et aL (1990). § Data taken from Marzachi et al (1991). 5" terminus of the three viruses: 5" GGUA---and 5' GGCA--- (Asamizu et al, 1985;Kudo et al, 1991). In the case of rice black-streaked dwarf virus (RBSDV), a Fijivirus, the terminal sequences of segment 10 have been found to be 5' NAGUUU-and -UGUC 3' (Azuhata et al, 1990).…”

Conserved terminal nucleotide sequences in the genome of rice black streaked dwarf virus

“…. -( U / C ) G A U 3' (Asamizu et al, 1985;Kudo et al, 1991). Therefore it is tempting to hypothesize that the conserved terminal oligonucleotide sequences of a virus are genus-specific.…”