Viruses Infecting Marine Mollusks

Renault, Tristan

doi:10.1002/9781119608370.ch8

Cited by 2 publications

(2 citation statements)

References 148 publications

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“…This promoter, we termed OsHV-1 promoter, was functional not only in scallop cells but also in HEK293 cells and zebrafish embryos. At present, no report exists for OsHV-1 infection in vertebrates, despite the worldwide infection of OsHV-1 in aquaculture bivalve species ( 60 , 61 ).…”

Section: Discussionmentioning

confidence: 99%

Gene delivery available in molluscan cells by strong promoter discovered from bivalve-infectious virus

Yoon

Konuma

et al. 2022

Proc. Natl. Acad. Sci. U.S.A.

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Understanding gene functions in marine invertebrates has been limited, largely due to the lack of suitable assay systems. Such a system requires investigative methods that are reproducible and can be quantitatively evaluated, such as a cell line, and a strong promoter that can drive high expression of a transgene. In this study, we established primary cell culture from a marine bivalve mollusc, Mizuhopecten yessoensis . Using scallop primary cells, we optimized electroporation conditions for transfection and carried out a luciferase-based promoter activity assay to identify strong promoter sequences that can drive expression of a gene of interest. We evaluated potential promoter sequences from genes of endogenous and exogenous origin and discovered a strong viral promoter derived from a bivalve-infectious virus, ostreid herpesvirus-1 (OsHV-1). This promoter, we termed OsHV-1 promoter, showed 24.7-fold and 16.1-fold higher activity than the cytomegalovirus immediate early (CMV IE) promoter and the endogenous EF1α promoter, the two most commonly used promoters in bivalves so far. Our GFP assays showed that the OsHV-1 promoter is active not only in scallop cells but also in HEK293 cells and zebrafish embryos. The OsHV-1 promoter practically enables functional analysis of marine molluscan genes, which can contribute to unveiling gene-regulatory networks underlying astonishing regeneration, adaptation, reproduction, and aging in marine invertebrates.

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Section: Discussionmentioning

confidence: 99%

Gene delivery available in molluscan cells by strong promoter discovered from bivalve-infectious virus

Yoon

Konuma

et al. 2022

Proc. Natl. Acad. Sci. U.S.A.

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“…To date, of the variety of revealed viruses, the best studied (including genomic characteristics) are only representatives of the families Herpesviridae, Iridoviridae and Birnaviridae, which cause great damage to the production of bivalve mollusks (such as oysters Crassostrea gigas, abalones Haliotis diversicolor supertexta, or scallops Chlamys farreri). Studies of highly pathogenic viruses of these families (especially the herpesviruses Ostreavirusostreidmalaco1 and Aurivirus haliotidmalaco1) made it possible to reveal the characteristics of these viruses, the mechanisms of virus-host interaction, and also to develop measures for diagnosis, prevention and reduction in morbidity [3,[19][20][21][22][23].…”

Section: Introductionmentioning

confidence: 99%

Viral Diversity in Samples of Freshwater Gastropods Benedictia baicalensis (Caenogastropoda: Benedictiidae) Revealed by Total RNA-Sequencing

Butina,

Zemskaya,

Bondaryuk

et al. 2023

IJMS

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Previously, the main studies were focused on viruses that cause disease in commercial and farmed shellfish and cause damage to food enterprises (for example, Ostreavirusostreidmalaco1, Aurivirus haliotidmalaco1 and Aquabirnavirus tellinae). Advances in high-throughput sequencing technologies have extended the studies to natural populations of mollusks (and other invertebrates) as unexplored niches of viral diversity and possible sources of emerging diseases. These studies have revealed a huge diversity of mostly previously unknown viruses and filled gaps in the evolutionary history of viruses. In the present study, we estimated the viral diversity in samples of the Baikal endemic gastropod Benedictia baicalensis using metatranscriptomic analysis (total RNA-sequencing); we were able to identify a wide variety of RNA-containing viruses in four samples (pools) of mollusks collected at three stations of Lake Baikal. Most of the identified viral genomes (scaffolds) had only distant similarities to known viruses or (in most cases) to metagenome-assembled viral genomes from various natural samples (mollusks, crustaceans, insects and others) mainly from freshwater ecosystems. We were able to identify viruses similar to those previously identified in mollusks (in particular to the picornaviruses Biomphalaria virus 1 and Biomphalaria virus 3 from the freshwater gastropods); it is possible that picorna-like viruses (as well as a number of other identified viruses) are pathogenic for Baikal gastropods. Our results also suggested that Baikal mollusks, like other species, may bioaccumulate or serve as a reservoir for numerous viruses that infect a variety of organisms (including vertebrates).

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Viruses Infecting Marine Mollusks

Cited by 2 publications

References 148 publications

Gene delivery available in molluscan cells by strong promoter discovered from bivalve-infectious virus

Gene delivery available in molluscan cells by strong promoter discovered from bivalve-infectious virus

Viral Diversity in Samples of Freshwater Gastropods Benedictia baicalensis (Caenogastropoda: Benedictiidae) Revealed by Total RNA-Sequencing

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