Visão geral sobre preservação da fertilidade feminina depois do câncer

Carvalho, Bruno Ramalho de; Rodrigues, Jhenifer Kliemchen; Campos, Jacira Ribeiro; Marinho, Ricardo Mello; Caetano, João Pedro Junqueira; Rosa‐e‐Silva, Ana Carolina Japur de Sá

doi:10.1016/j.recli.2015.04.003

Cited by 9 publications

(22 citation statements)

References 53 publications

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“…However, chemo and radiation therapy used in cancer treatment may compromise future fertility (Smitz et al ., 2010; González et al ., 2012; Donnez & Dolmans, 2015; Larsen et al ., 2003; Carvalho et al ., 2014; Rodrigues et al ., 2015; Frydman & Grynberg, 2016) on account of their negative effects on ovarian follicular reserve, in addition to possibly causng infertility or even amenorrhea with hypoestrogenism symptoms (González et al ., 2012). Oocyte cryopreservation is one of the most widely used strategies to preserve the fertility of cancer patients today, with potentially significant pregnancy rates after thawing and IVF (Alvarez & Ramanathan, 2018).…”

Section: Introductionmentioning

confidence: 99%

Oocyte cryopreservation for future fertility: comparison of ovarian response between cancer and non-cancer patients

Moraes¹,

Marinho²,

Campos³

et al. 2019

JBRA Assisted Reproduction

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Objective: This study aimed to assess whether a diagnosis of cancer interferes with ovarian function prior to the treatment of the disease. Methods: This observational retrospective study used data from medical records of ovarian stimulation cycles performed for purposes of oocyte cryopreservation. Results: The included patients had a mean age of 35.13±3.72 years and 51.6% of them were aged between 36 and 40 years. More than half of the patients (57.6%) were single and 82.1% had a normal body mass index (BMI). Most women had not become pregnant (85.5%) or had babies (95.1%) or miscarriages (89.6%) prior to cryopreservation. The mean number of oocytes obtained from non-cancer patients was 11.4±8, while for cancer patients the number was 13.8±9. The mean number of frozen mature oocytes was 9.7±7 for the non-cancer group and 11.2±7.2 for the cancer group. The majority (63.1%) of the patients had up to 10 oocytes frozen per cycle. Breast cancer had the highest incidence among the included patients. There was no significant difference in ovarian response between patients with different types of cancer. Conclusion: The number of harvested and frozen oocytes from cancer and non-cancer patients indicated that in the two groups response to ovarian stimulation was similar.

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Section: Introductionmentioning

confidence: 99%

Oocyte cryopreservation for future fertility: comparison of ovarian response between cancer and non-cancer patients

Moraes¹,

Marinho²,

Campos³

et al. 2019

JBRA Assisted Reproduction

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“…1 The main treatments are those used in oncology (surgery, chemotherapy, or radiotherapy). 2 However, with early diagnosis and the newly available therapies, most of these patients will have a good chance of cure or long survival, 3 but may have impaired fertility.…”

Section: Introductionmentioning

confidence: 99%

Comparison between Slow Freezing and Vitrification in Terms of Ovarian Tissue Viability in a Bovine Model

Campos

Guedes

Rodrigues³

et al. 2016

Rev Bras Ginecol Obstet

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Objective To assess the viability of bovine ovarian tissue after cryopreservation through either slow freezing or vitrification, and to compare it to that of control tissue by performing morphological analyses. Methods The study included 20 bovine ovarian cortex fragments that were divided into control, vitrification, and slow freezing groups. Each group consisted of four fragments of the same ovary, two fixed without cultivation, and two fixed with cultivation. Tissues were evaluated based on follicular morphology immediately after heating and after 7 days of culture, and compared with the control group. Results A total of 240 fragments were analyzed, generating a sample of 1,344 follicles without cultivation and 552 with cultivation. When the non-cultivated samples were classified as non-atretic follicles, 572 were found in the control group, 289 in the vitrification group, and 373 in the slow freezing group, showing no significant differences. When classified as atretic, 46 follicles were found in the control group, 23 in the vitrification group, and 41 in the slow freezing group, also showing no statistical difference. In the post-culture sample, an evolution of the follicular stages could be observed. This finding was important to support that the follicles considered non-atretic in the non-cultivated group were actually viable in the morphological evaluation. Conclusion With no differences between the protocols, vitrification was shown to be an advanced and alternative method for patients who will undergo treatments that

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“…Nevertheless, the chemotherapy, the radiotherapy or even the surgery performed in the treatment may compromise their future fertility. [1][2][3][4] Cryopreservation of oocytes and embryos are alternatives for young adult women who can wait $ 2-3 weeks for ovarian stimulation and oocyte collection.…”

Section: Introductionmentioning

confidence: 99%

“…The only option for the preservation of the fertility of the patients that require immediate treatment, such as children and prepubescent adolescents, is the cryopreservation of ovarian tissue. [1][2][3][4][5][6] Studies have shown that freezing ovarian tissue fragments may preserve the gametes and also the ability to produce hormones. 2,7,8 The collection procedure can be readily programmed at any phase of the cycle, and the patient's exposure to hormone treatment is not required.…”

Section: Introductionmentioning

confidence: 99%

“…4 The maturation of existing ovarian follicles in cryopreserved ovarian tissue fragments in the laboratory to obtain mature oocytes to be fertilized in vitro would avoid the risk of cancer recurrence. [3][4][5] In vitro follicular maturation techniques have been developed for humans, but their improvement becomes difficult due to the enormous difficulty in obtaining tissue and due to ethical issues. 12 The results of human studies are still limited to the study by Amorim et al, 13 in which pre-antral ovarian follicles survived for a period of 7 days after cryopreservation.…”

Section: Introductionmentioning

confidence: 99%

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Follicle Viability after Vitrification of Bovine Ovarian Tissue

Guedes

Rodrigues

Campos

et al. 2017

Rev Bras Ginecol Obstet

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Keywords► in vitro follicular maturation ► fertility preservation ► vitrification AbstractPurpose The present study aimed to evaluate the impact of vitrification on the viability of follicles using a three-dimensional (3D) in vitro culture. Methods Bovine ovarian tissue samples (n ¼ 5) obtained from slaughterhouses were utilized. The cortex was cut into small fragments of 2 Â 3 Â 0.5 mm using a tissue slicer. From these fragments, secondary follicles were first isolated by mechanical and enzymatic methods, then encapsulated in alginate gel and individually cultured for 20 days. Additional fragments of the same ovarian tissue were vitrified in a solution containing 25% glycerol and 25% ethylene glycol. After warming, the follicles underwent the same follicular isolation process that was performed for the fresh follicles. Results A total of 61 follicles were isolated, 51 from fresh ovarian tissue, and 10 from vitrified tissue. After the culture, the vitrified and fresh follicles showed 20% and 43.1% survival rates respectively (p ¼ 0.290), with no significant differences. At the end of the culture, there were no significant differences in follicular diameter between the vitrified (422.93 AE 85.05 µm) and fresh (412.99 AE 102.55 µm) groups (p ¼ 0.725). Fresh follicles showed higher mean rate of antrum formation when compared with vitrified follicles (47.1% and 20.0% respectively), but without significant difference (p ¼ 0.167). Conclusions The follicles were able to develop, grow and form antrum in the 3D system after vitrification, despite the lower results obtained with the fresh tissue. ResumoObjetivo O presente estudo teve como objetivo avaliar o impacto da vitrificação na viabilidade dos folículos utilizando a cultura in vitro tridimensional (3D). Métodos Foi utilizado tecido ovariano bovino (n ¼ 5) obtido de abatedouros. O córtex foi cortado em pequenos fragmentos de 2 Â 3 Â 0,5 mm, utilizando o tissue slicer e a partir destes fragmentos foram isolados folículos secundários por meio de

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Visão geral sobre preservação da fertilidade feminina depois do câncer

Cited by 9 publications

References 53 publications

Oocyte cryopreservation for future fertility: comparison of ovarian response between cancer and non-cancer patients

Oocyte cryopreservation for future fertility: comparison of ovarian response between cancer and non-cancer patients

Comparison between Slow Freezing and Vitrification in Terms of Ovarian Tissue Viability in a Bovine Model

Follicle Viability after Vitrification of Bovine Ovarian Tissue

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