Viscoelastic Properties Measurement of Human Lymphocytes by Atomic Force Microscopy Based on Magnetic Beads Cell Isolation

Li, Mi; Liu, Lianqing; Xiao, Xiubin; Xi, Ning; Wang, Yuechao

doi:10.1109/tnb.2016.2547639

Cited by 39 publications

(33 citation statements)

References 64 publications

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“…The conical tip can discriminate the diverse mechanical properties of different sub-cellular structures (such as cytoskeleton [35]), which facilitates investigating the relationship between cell structures and cell mechanics [44]. While the spherical tip can better represent the mechanical properties of the whole cell [45]. The loading force F is calculated according to Hooke's law:…”

Section: A Cellular Elastic Propertiesmentioning

confidence: 99%

Atomic Force Microscopy in Characterizing Cell Mechanics for Biomedical Applications: A Review

Dang

Liu

et al. 2017

IEEE Trans.on Nanobioscience

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104

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-Cell mechanics is a novel label-free biomarker for indicating cell states and pathological changes. The advent of atomic force microscopy (AFM) provides a powerful tool for quantifying the mechanical properties of single living cells in aqueous conditions. The wide use of AFM in characterizing cell mechanics in the past two decades has yielded remarkable novel insights in understanding the development and progression of certain diseases, such as cancer, showing the huge potential of cell mechanics for practical applications in the field of biomedicine. In this paper, we reviewed the utilization of AFM to characterize cell mechanics. First, the principle and method of AFM single-cell mechanical analysis was presented, along with the mechanical responses of cells to representative external stimuli measured by AFM. Next, the unique changes of cell mechanics in two types of physiological processes (stem cell differentiation, cancer metastasis) revealed by AFM were summarized. After that, the molecular mechanisms guiding cell mechanics were analyzed. Finally the challenges and future directions were discussed.

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Section: A Cellular Elastic Propertiesmentioning

confidence: 99%

Atomic Force Microscopy in Characterizing Cell Mechanics for Biomedical Applications: A Review

Dang

Liu

et al. 2017

IEEE Trans.on Nanobioscience

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104

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“…Primary B lymphocytes were isolated from the peripheral anticoagulated blood of healthy volunteers (Li et al, 2016a). First, the peripheral blood mononuclear cells (PBMCs) were obtained by density gradient centrifugation.…”

Section: Primary B Lymphocyte Isolationmentioning

confidence: 99%

“…The AFM tip was moved to the central area of cell under the guidance of optical microscopy ( Figure 7B). Then the AFM tip was controlled to indent the cells in the approach-reside-retract mode to measure the elastic and viscoelastic properties of cells (Li et al, 2016a); the process of measuring the cellular elastic and viscoelastic properties is shown in Figure 7D. During the approach-reside-retract mode, the AFM tip first approached (at a loading rate 8 μm/s) and touched the cell until the maximal loading force (5 nN) was achieved.…”

Section: Afm Indentingmentioning

confidence: 99%

“…The spherical probe was fabricated by gluing a sphere to an AFM cantilever according to an established procedure of AFM micromanipulations (Li et al, 2016a). Briefly, a drop of two-part epoxy adhesive was placed on a glass slide and a drop of sphere solution was placed at another position on the same glass slide, which was then put on the stage of AFM.…”

Section: Spherical Probe Fabricationmentioning

confidence: 99%

“…The latter result can be attributed to the diverse viscoelastic behaviors of cytoplasm, whose flow enables the relaxation process of cells after indentation by the AFM tip (Moeendarbary et al, 2013); cytoplasm comprises heterogeneous components such as actin filaments, intermediate filaments, microtubules, nucleus, and organelles (Kirmizis and Logothetidis, 2010). Hence, a two-order Maxwell model can match the experimental stress relaxation curves well (Li et al, 2016a;Li et al, 2016b). Figure 2 shows the statistical contrast of elastic and viscoelastic properties between MCF-10A cells and MCF-7 cells measured by AFM approach-reside-retract experiments.…”

Section: Introductionmentioning

confidence: 96%

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Atomic force microscopy studies on cellular elastic and viscoelastic properties

Liu

et al. 2017

Sci. China Life Sci.

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In this work, a method based on atomic force microscopy (AFM) approach-reside-retract experiments was established to simultaneously quantify the elastic and viscoelastic properties of single cells. First, the elastic and viscoelastic properties of normal breast cells and cancerous breast cells were measured, showing significant differences in Young's modulus and relaxation times between normal and cancerous breast cells. Remarkable differences in cellular topography between normal and cancerous breast cells were also revealed by AFM imaging. Next, the elastic and viscoelasitc properties of three other types of cell lines and primary normal B lymphocytes were measured; results demonstrated the potential of cellular viscoelastic properties in complementing cellular Young's modulus for discerning different states of cells. This research provides a novel way to quantify the mechanical properties of cells by AFM, which allows investigation of the biomechanical behaviors of single cells from multiple aspects.

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Nanoparticle–Cell Interaction: A Cell Mechanics Perspective

et al. 2018

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Progress in the field of nanoparticles has enabled the rapid development of multiple products and technologies; however, some nanoparticles can pose both a threat to the environment and human health. To enable their safe implementation, a comprehensive knowledge of nanoparticles and their biological interactions is needed. In vitro and in vivo toxicity tests have been considered the gold standard to evaluate nanoparticle safety, but it is becoming necessary to understand the impact of nanosystems on cell mechanics. Here, the interaction between particles and cells, from the point of view of cell mechanics (i.e., bionanomechanics), is highlighted and put in perspective. Specifically, the ability of intracellular and extracellular nanoparticles to impair cell adhesion, cytoskeletal organization, stiffness, and migration are discussed. Furthermore, the development of cutting-edge, nanotechnology-driven tools based on the use of particles allowing the determination of cell mechanics is emphasized. These include traction force microscopy, colloidal probe atomic force microscopy, optical tweezers, magnetic manipulation, and particle tracking microrheology.

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Viscoelastic Properties Measurement of Human Lymphocytes by Atomic Force Microscopy Based on Magnetic Beads Cell Isolation

Cited by 39 publications

References 64 publications

Atomic Force Microscopy in Characterizing Cell Mechanics for Biomedical Applications: A Review

Atomic Force Microscopy in Characterizing Cell Mechanics for Biomedical Applications: A Review

Atomic force microscopy studies on cellular elastic and viscoelastic properties

Nanoparticle–Cell Interaction: A Cell Mechanics Perspective

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