Viscoelastic properties of acid‐ and alkaline‐treated human dermis: a correlation between total surface charge and elastic modulus

Seehra, Gurinder P.; Silver, Frederick H.

doi:10.1111/j.0909-752x.2006.00150.x

Cited by 27 publications

(42 citation statements)

References 18 publications

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“…Changes in the number of glutamic acid, threonine, serine, and threonine residues could cause changes in the surface charge of collagen molecules (49,53), whereas changes in the quantity of valine, alanine, and phenylalanine residues could infl uence the level of collagen helix rigidity (49). In addition, changes in glycine residues could affect the number of Arg-GlyAsp domains responsible for the processes of cell adhesion to collagen structures (50)(51)(52).…”

Section: Discussionmentioning

confidence: 99%

CYP2E1 Testis Expression and Alcoholmediated Changes of Rat Spermatogenesis Indices and Type I Collagen

Shayakhmetova

Bondarenko

Коваленко

et al. 2013

Archives of Industrial Hygiene and Toxicology

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This study is a complex investigation of alcohol-mediated changes in CYP2E1 mRNA and protein expression in the testes, as well as spermatogenesis indices and type I collagen amino acid contents, in male rats. Wistar albino male rats were divided into two groups: I -control (intact animals), II -experimental (chronic alcoholism, exposure to a 15 % ethanol aqueous solution during 150 days). The destructive changes in the spermatogenic epithelium were accompanied by a decrease in sperm number and motility time. CYP2E1 mRNA and protein expression were elevated in the testes 3 and 1.4 times, respectively. Also, signifi cantly lower contents of lysine, glutamic acid, serine, proline, alanine, valine, and phenylalanine residues accompanied by an increase of hydroxyproline, glycine, and threonine residue contents were detected in the skin type I collagen of the experimental group. Chronic ethanol consumption caused testicular failure along with an overexpression of CYP2E1 mRNA and protein in the testes as well as quantitative changes in type I collagen amino acid contents. The profound alcohol-mediated changes in collagen type I amino acid contents may have affected the spermatogenic epithelium state. The modulation of testicular cytochrome P450 2E1 mRNA and protein expression could change the functioning of this isozyme in target organs and take part in the mechanism of ethanol gonadotoxicity.

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Section: Discussionmentioning

confidence: 99%

CYP2E1 Testis Expression and Alcoholmediated Changes of Rat Spermatogenesis Indices and Type I Collagen

Shayakhmetova

Bondarenko

Коваленко

et al. 2013

Archives of Industrial Hygiene and Toxicology

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“…In summary, stress-strain protocols reveal that soft tissues may be characterized by a history-independent elastic component and a history-dependent viscous component (137,407,420,421). Under certain conditions, a straindependent plastic component can also be revealed.…”

Section: Basic Spring and Dashpot Constitutive Equationsmentioning

confidence: 97%

“…Although the only stress information obtained from an instantaneous strain-step are the initial and final values, this, along with the subsequent stress relaxation to steady state, are sufficient when applying a sequential series of stretch strain-step/strain-clamps over a long strain-range to separate the stress response into elastic and viscous components ( Fig. 13) for tissues such as artery, vein (420,421), and skin (407).…”

Section: Basic Spring and Dashpot Constitutive Equationsmentioning

confidence: 99%

Mechanics of Vascular Smooth Muscle

Ratz

2015

Comprehensive Physiology

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Vascular smooth muscle (VSM; see Table 1 for a list of abbreviations) is a heterogeneous biomaterial comprised of cells and extracellular matrix. By surrounding tubes of endothelial cells, VSM forms a regulated network, the vasculature, through which oxygenated blood supplies specialized organs, permitting the development of large multicellular organisms. VSM cells, the engine of the vasculature, house a set of regulated nanomotors that permit rapid stress-development, sustained stress-maintenance and vessel constriction. Viscoelastic materials within, surrounding and attached to VSM cells, comprised largely of polymeric proteins with complex mechanical characteristics, assist the engine with countering loads imposed by the heart pump, and with control of relengthening after constriction. The complexity of this smart material can be reduced by classical mechanical studies combined with circuit modeling using spring and dashpot elements. Evaluation of the mechanical characteristics of VSM requires a more complete understanding of the mechanics and regulation of its biochemical parts, and ultimately, an understanding of how these parts work together to form the machinery of the vascular tree. Current molecular studies provide detailed mechanical data about single polymeric molecules, revealing viscoelasticity and plasticity at the protein domain level, the unique biological slip-catch bond, and a regulated two-step actomyosin power stroke. At the tissue level, new insight into acutely dynamic stress-strain behavior reveals smooth muscle to exhibit adaptive plasticity. At its core, physiology aims to describe the complex interactions of molecular systems, clarifying structure-function relationships and regulation of biological machines. The intent of this review is to provide a comprehensive presentation of one biomachine, VSM.

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“…Human decellularized dermis was obtained from allograft tissue as described previously 5,6,[9][10][11] (Table 1). Decellularized human dermal samples were tested after immersion in phosphate buffer solution as described elsewhere.…”

Section: Sample Preparationmentioning

confidence: 99%

“…5,6,[9][10][11] Recently we have reported the use of optical coherence tomography (OCT) and vibrational analysis to non-invasively and non-destructively measure the mechanical properties of decellularized human dermis in vitro 5,6 and human skin in vivo 12 this technique measures the resonant frequency of a tissue or polymer and then converts the resonant frequency into a modulus. In addition, we have reported that moduli measurements made at the resonant frequency of ECMs and silicone rubber are quasi-elastic, since the viscous contribution to the mechanical behavior is as low as 2% to 4%.…”

Section: Introductionmentioning

confidence: 99%

Vibrational Analysis of Extracellular Matrix Scaffolds: Comparison of Skin, Dermis, Cartilage and Subchondral Bone Using Oct and Vibrational Analysis

Silver¹

2017

ATROA

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Viscoelastic properties of acid‐ and alkaline‐treated human dermis: a correlation between total surface charge and elastic modulus

Cited by 27 publications

References 18 publications

CYP2E1 Testis Expression and Alcoholmediated Changes of Rat Spermatogenesis Indices and Type I Collagen

CYP2E1 Testis Expression and Alcoholmediated Changes of Rat Spermatogenesis Indices and Type I Collagen

Mechanics of Vascular Smooth Muscle

Vibrational Analysis of Extracellular Matrix Scaffolds: Comparison of Skin, Dermis, Cartilage and Subchondral Bone Using Oct and Vibrational Analysis

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