Viscoelastic properties of doxorubicin-treated HT-29 cancer cells by atomic force microscopy: the fractional Zener model as an optimal viscoelastic model for cells

Rodríguez-Nieto, Maricela; Mendoza-Flores, Priscila; García-Ortiz, David; Montes-de-Oca, Luis M.; Mendoza-Villa, Marco Antonio; Barrón-González, Porfiria; Espinosa, G. P.; Menchaca, Jorge Luis

doi:10.1007/s10237-019-01248-9

Cited by 11 publications

(9 citation statements)

References 56 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Among these parameters, the elasticity of cells is discussed to be the most suitable biomarker for cancer [66]. Estimated elastic moduli in this study are in the same order of magnitude as expected for eukaryotic cells, varying between several hundred Pascal to 10 kPa [3,25], and are comparable to previous studies on cancerous cells with different tissue origins [8]. Yet, comparison of absolute Young's moduli determined in different studies is known to be difficult, as its measurement is highly dependent on several factors such as tip geometry and coating, as well as cell experimental conditions [8].…”

Section: Discussionsupporting

confidence: 52%

“…Biomechanical properties have already been studied for various tissues [20], among them the gastrointestinal tract as well as the colon [21], though this technique has been predominantly applied to study non-pathological tissues [22,23], and less to differentiate between healthy and deteriorated, abnormal tissue from colon biopsies. Data on biomechanical properties of colon carcinoma cell lines measured with AFM are scarce, both regarding their reaction before and after treatment with nutritive as well as pharmacological substances [24,25].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Time- and Zinc-Related Changes in Biomechanical Properties of Human Colorectal Cancer Cells Examined by Atomic Force Microscopy

Maares

Keil

Löher

et al. 2020

Biology

View full text Add to dashboard Cite

Monitoring biomechanics of cells or tissue biopsies employing atomic force microscopy (AFM) offers great potential to identify diagnostic biomarkers for diseases, such as colorectal cancer (CRC). Data on the mechanical properties of CRC cells, however, are still scarce. There is strong evidence that the individual zinc status is related to CRC risk. Thus, this study investigates the impact of differing zinc supply on the mechanical response of the in vitro CRC cell lines HT-29 and HT-29-MTX during their early proliferation (24–96 h) by measuring elastic modulus, relaxation behavior, and adhesion factors using AFM. The differing zinc supply severely altered the proliferation of these cells and markedly affected their mechanical properties. Accordingly, zinc deficiency led to softer cells, quantitatively described by 20–30% lower Young’s modulus, which was also reflected by relevant changes in adhesion and rupture event distribution compared to those measured for the respective zinc-adequate cultured cells. These results demonstrate that the nutritional zinc supply severely affects the nanomechanical response of CRC cell lines and highlights the relevance of monitoring the zinc content of cancerous cells or biopsies when studying their biomechanics with AFM in the future.

show abstract

Section: Discussionsupporting

confidence: 52%

Section: Introductionmentioning

confidence: 99%

Time- and Zinc-Related Changes in Biomechanical Properties of Human Colorectal Cancer Cells Examined by Atomic Force Microscopy

Maares

Keil

Löher

et al. 2020

Biology

View full text Add to dashboard Cite

show abstract

“…Storage and loss moduli must then both be reported when characterizing mechanical properties, as cells respond to both elastic and viscous parts of their naturally viscoelastic substrates modifying their internal structures and nuclear envelope [25]. All this has been demonstrated to be of particular importance in tissue engineering [26], cancer research for treatment [27] or diagnostics [28].…”

Section: Introductionmentioning

confidence: 99%

Determination by Relaxation Tests of the Mechanical Properties of Soft Polyacrylamide Gels Made for Mechanobiology Studies

et al. 2021

View full text Add to dashboard Cite

Following the general aim of recapitulating the native mechanical properties of tissues and organs in vitro, the field of materials science and engineering has benefited from recent progress in developing compliant substrates with physical and chemical properties similar to those of biological materials. In particular, in the field of mechanobiology, soft hydrogels can now reproduce the precise range of stiffnesses of healthy and pathological tissues to study the mechanisms behind cell responses to mechanics. However, it was shown that biological tissues are not only elastic but also relax at different timescales. Cells can, indeed, perceive this dissipation and actually need it because it is a critical signal integrated with other signals to define adhesion, spreading and even more complicated functions. The mechanical characterization of hydrogels used in mechanobiology is, however, commonly limited to the elastic stiffness (Young’s modulus) and this value is known to depend greatly on the measurement conditions that are rarely reported in great detail. Here, we report that a simple relaxation test performed under well-defined conditions can provide all the necessary information for characterizing soft materials mechanically, by fitting the dissipation behavior with a generalized Maxwell model (GMM). The simple method was validated using soft polyacrylamide hydrogels and proved to be very useful to readily unveil precise mechanical properties of gels that cells can sense and offer a set of characteristic values that can be compared with what is typically reported from microindentation tests.

show abstract

“…While both drugs are well known to inhibit DNA synthesis [ 39 , 41 ], a number of action mechanisms have been proposed for drug-mediated cell deaths. For example, doxorubicin was shown to intercalate nucleic acids and participate in depolymerization of actin filaments, destabilization of cytoskeletal structures, thus reducing the biomechanical strength of a cell, such as stiffness [ 42 , 43 ]. A significant decrease of Young’s modulus and a change in cellular morphology of lung cancer cells treated by methotrexate, classified as an antimetabolite like gemcitabine, were also previously reported [ 44 ].…”

Section: Resultsmentioning

confidence: 99%

Dynamic cellular biomechanics in responses to chemotherapeutic drug in hypoxia probed by atomic force spectroscopy

et al. 2021

View full text Add to dashboard Cite

The changes in cellular structure play an important role in cancer cell development, progression, and metastasis.By exploiting single-cell, force spectroscopy methods, we probed biophysical and biomechanical kinetics (stiffness, morphology, roughness, adhesion) of brain, breast, prostate, and pancreatic cancer cells with standard chemotherapeutic drugs in normoxia and hypoxia over 12-24 hours. After exposure to the drugs, we found that brain, breast, and pancreatic cancer cells became approximately 55-75% less stiff, while prostate cancer cells became more stiff, due to either drug-induced disruption or reinforcement of cytoskeletal structure. However, the rate of the stiffness change decreased up to 2-folds in hypoxia, suggesting a correlation between cellular stiffness and drug resistance of cancer cells in hypoxic tumor microenvironment. Also, we observed significant changes in the cell body height, surface roughness, and cytoadhesion of cancer cells after exposure to drugs, which followed the trend of stiffness. Our results show that a degree of chemotherapeutic drug effects on biomechanical and biophysical properties of cancer cells is distinguishable in normoxia and hypoxia, which are correlated with alteration of cytoskeletal structure and integrity during drug-induced apoptotic process.

show abstract

Viscoelastic properties of doxorubicin-treated HT-29 cancer cells by atomic force microscopy: the fractional Zener model as an optimal viscoelastic model for cells

Cited by 11 publications

References 56 publications

Time- and Zinc-Related Changes in Biomechanical Properties of Human Colorectal Cancer Cells Examined by Atomic Force Microscopy

Time- and Zinc-Related Changes in Biomechanical Properties of Human Colorectal Cancer Cells Examined by Atomic Force Microscopy

Determination by Relaxation Tests of the Mechanical Properties of Soft Polyacrylamide Gels Made for Mechanobiology Studies

Dynamic cellular biomechanics in responses to chemotherapeutic drug in hypoxia probed by atomic force spectroscopy

Contact Info

Product

Resources

About