Viscoelastic Properties of Healthy Human Artery Measured in Saline Solution by AFM-Based Indentation Technique

Lundkvist, A S; Lilleodden, Erica T.; Siekhaus, Wigbert J.; Kinney, J.H.; Pruitt, Lisa A.; Balooch, M.

doi:10.1557/proc-436-353

Cited by 24 publications

(20 citation statements)

References 9 publications

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“…This is similar to values reported for corneal basement membranes as well as Matrigel, a basement membrane-like complex [34,35]. There are a few publications reporting markedly higher values from 100 + kPa to greater than 1 MPa [14,36]. It is likely that the differences in values for modulus are related to differences in the analytic measurement methods used [5].…”

Section: Discussionsupporting

confidence: 80%

The role of substratum compliance of hydrogels on vascular endothelial cell behavior

Wood¹,

Shah²,

McKee³

et al. 2011

Biomaterials

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Cardiovascular disease (CVD) remains a leading cause of death both within the United States (US) as well as globally. In 2006 alone, over one-third of all deaths in the US were attributable to CVD. The high prevalence, mortality, morbidity, and socioeconomic impact of CVD has motivated a significant research effort; however, there remain significant knowledge gaps regarding disease onset and progression as well as pressing needs for improved therapeutic approaches. One critical area of research that has received limited attention is the role of biophysical cues on the modulation of endothelial cell behaviors; specifically, the impact of local compliance, or the stiffness, of the surrounding vascular endothelial extracellular matrix. In this study, the impact of substratum compliance on the modulation of cell behaviors of several human primary endothelial cell types, representing different anatomic sites and differentiation states in vivo, were investigated. Substrates used within our studies span the range of compliance that has been reported for the vascular endothelial basement membrane. Differences in substratum compliance had a profound impact on cell attachment, spreading, elongation, proliferation, and migration. In addition, each cell population responded differentially to changes in substratum compliance, documenting endothelial heterogeneity in the response to biophysical cues. These results demonstrate the importance of incorporating substratum compliance in the design of in vitro experiments as well as future prosthetic design. Alterations in vascular substratum compliance directly influence endothelial cell behavior and may participate in the onset and/or progression of CVDs.

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Section: Discussionsupporting

confidence: 80%

The role of substratum compliance of hydrogels on vascular endothelial cell behavior

Wood¹,

Shah²,

McKee³

et al. 2011

Biomaterials

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“…The large variability in measurements, spanning the moduli of fibrous tissue and bone, was not expected. While the wide range of Mi:M values associated with the calcifications (1–28) suggests that some of the observed spread in reduced modulus values may be due to variations in tissue composition or incomplete mineralization, other sources of variability associated with the sample preparation methods are also thought to have played a role. Calcifications were dissected with razor blades under a dissecting microscope, and no polishing or microtoming was performed.…”

Section: Discussionmentioning

confidence: 99%

“…Nanoindentation of soft tissues requires modifications to standard indentation techniques, such as the use of a blunt spherical tip rather than a sharp Berkovich tip for indentations, sample hydration during testing, and the implementation of software corrections for large displacements 17. Despite these complications, preliminary micro‐ and nanoindentation studies have validated this technique for application to vascular tissues 18–21…”

Section: Introductionmentioning

confidence: 99%

Nanomechanical properties of calcification, fibrous tissue, and hematoma from atherosclerotic plaques

Ebenstein

Coughlin

Chapman

et al. 2008

J Biomedical Materials Res

138

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Clinical events such as heart attack and stroke can be caused by the rupture of atherosclerotic plaques in artery walls. Computational modeling is often used to better understand atherosclerotic disease progression to identify "vulnerable" plaques (i.e., those likely to rupture) and to tailor treatments according to tissue composition. However, because of the heterogeneity of plaque tissue, there are limited data available on the material properties of individual plaque constituents. The goal of this study was to use nanoindentation to measure the mechanical properties of blood clots, fibrous tissue, partially calcified fibrous tissue, and bulk calcifications from human atherosclerotic plaque tissue. Fourier transform infrared (FTIR) spectroscopy was used to quantify the amount of mineral and lipid in each tissue region tested. The results demonstrate that the stiffness of plaque tissue increases with increasing mineral content. In addition, by providing the first experimental data on atherosclerotic calcifications, these data show that some of the estimated modulus values commonly used in computational models greatly underestimate the stiffness of the fully calcified tissue.

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“…Topographically, the endothelial basement membrane is comprised of nano and submicron scale features arranged in a felt-like meshwork [28,96]. The local compliance (Young’s modulus) of the native basement membrane of vasculature is between 8–70 kPa, though some researchers have reported values greater than 100 kPa and one report is in the MPa range, though this tissue was frozen prior to analysis and the reported modulus is not consistent with the other literature [23,97,98,99,100]. These discrepancies may be the result of vascular stiffening or the differences in the acquisition and analysis of atomic force microscopic data.…”

Section: Basmenet Membranementioning

confidence: 99%

Biophysical Cueing and Vascular Endothelial Cell Behavior

et al. 2010

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Human vascular endothelial cells (VEC) line the vessels of the body and are critical for the maintenance of vessel integrity and trafficking of biochemical cues. They are fundamental structural elements and are central to the signaling environment. Alterations in the normal functioning of the VEC population are associated with a number of vascular disorders among which are some of the leading causes of death in both the United States and abroad. VECs attach to their underlying stromal elements through a specialization of the extracellular matrix, the basement membrane. The basement membrane provides signaling cues to the VEC through its chemical constituents, by serving as a reservoir for cytoactive factors and through its intrinsic biophysical properties. This specialized matrix is composed of a topographically rich 3D felt-like network of fibers and pores on the nano (1–100 nm) and submicron (100–1,000 nm) size scale. The basement membrane provides biophysical cues to the overlying VECs through its intrinsic topography as well as through its local compliance (relative stiffness). These biophysical cues modulate VEC adhesion, migration, proliferation, differentiation, and the cytoskeletal signaling network of the individual cells. This review focuses on the impact of biophysical cues on VEC behaviors and demonstrates the need for their consideration in future vascular studies and the design of improved prosthetics.

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Viscoelastic Properties of Healthy Human Artery Measured in Saline Solution by AFM-Based Indentation Technique

Cited by 24 publications

References 9 publications

The role of substratum compliance of hydrogels on vascular endothelial cell behavior

The role of substratum compliance of hydrogels on vascular endothelial cell behavior

Nanomechanical properties of calcification, fibrous tissue, and hematoma from atherosclerotic plaques

Biophysical Cueing and Vascular Endothelial Cell Behavior

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