Viscoelasticity of Thin Biomolecular Films: A Case Study on Nucleoporin Phenylalanine-Glycine Repeats Grafted to a Histidine-Tag Capturing QCM-D Sensor

Eisele, Nico B.; Andersson, Fredrik I.; Frey, Steffen; Richter, Ralf P.

doi:10.1021/bm300577s

Cited by 86 publications

(118 citation statements)

References 54 publications

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“…QCM-D measurements were performed as described in detail elsewhere (67, 68). The QCM-D response is sensitive to the mass (including coupled water) and the viscoelastic properties of the surface adlayer.…”

Section: Methodsmentioning

confidence: 99%

Incorporation of Pentraxin 3 into Hyaluronan Matrices Is Tightly Regulated and Promotes Matrix Cross-linking

Baranova

Inforzato

Briggs

et al. 2014

Journal of Biological Chemistry

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Background: The proteins pentraxin 3 (PTX3) and TNF-stimulated gene-6 (TSG-6) and the proteoglycan inter-α-inhibitor (IαI) are known to be involved in the stabilization of hyaluronan (HA)-rich extracellular matrices.Results: PTX3 incorporation into HA matrices is tightly regulated.Conclusion: PTX3, TSG-6, and IαI are sufficient to cross-link HA matrices.Significance: The results provide mechanistic insights into the regulation of HA-protein interactions.

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Section: Methodsmentioning

confidence: 99%

Incorporation of Pentraxin 3 into Hyaluronan Matrices Is Tightly Regulated and Promotes Matrix Cross-linking

Baranova

Inforzato

Briggs

et al. 2014

Journal of Biological Chemistry

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“…Initially the film density ρ was also fixed to 1.0 g.cm -3 , without this affecting the generality of the modeling (see below). Details of the fitting procedure have been described previously, 21 and the specified errors represent a confidence level of one standard deviation (68%).…”

Section: Technicalmentioning

confidence: 99%

Sensor Based on Aptamer Folding to Detect Low-Molecular Weight Analytes

et al. 2015

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Aptamers have emerged as promising biorecognition elements in the development of biosensors. The present work focuses on the application of quartz crystal microbalance with dissipation monitoring (QCM-D) for the enantioselective detection of a low molecular weight target molecule (less than 200 Da) by aptamer-based sensors. While QCM-D is a powerful technique for label-free, real-time characterization and quantification of molecular interactions at interfaces, the detection of small molecules interacting with immobilized receptors still remains a challenge. In the present study, we take advantage of the aptamer conformational changes upon the target binding that induces displacement of water acoustically coupled to the sensing layer. As a consequence, this phenomenon leads to a significant enhancement of the detection signal. The methodology is exemplified with the enantioselective recognition of a low molecular weight model compound, L-tyrosinamide (L-Tym). QCM-D monitoring of L-Tym interaction with the aptamer monolayer leads to an appreciable signal that can be further exploited for analytical purposes or thermodynamics studies. Furthermore, in situ combination of QCM-D with spectroscopic ellipsometry unambiguously demonstrates that the conformational change induces a nanometric decrease of the aptamer monolayer thickness. Since QCM-D is sensitive to the whole mass of the sensing layer including water that is acoustically coupled, a decrease in thickness of the highly hydrated aptamer layer induces a sizable release of water that can be easily detected by QCM-D.

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“…The system was operated in flow mode with a flow rate of typically 10 mL/min using a peristaltic pump (ISM935C, Ismatec, Zurich, Switzerland [30,31] as described in detail elsewhere [32]. These thickness values are provided as average ± standard deviation from at least three independent experiments.…”

Section: Quartz Crystal Microbalance With Dissipation Monitoring (Qcm-d)mentioning

confidence: 99%

Well-defined biomimetic surfaces to characterize glycosaminoglycan-mediated interactions on the molecular, supramolecular and cellular levels

et al. 2014

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a b s t r a c tGlycosaminoglycans (GAGs) are ubiquitously present at the cell surface and in extracellular matrix, and crucial for matrix assembly, cellecell and cell-matrix interactions. The supramolecular presentation of GAG chains, along with other matrix components, is likely to be functionally important but remains challenging to control and to characterize, both in vivo and in vitro. We present a method to create welldefined biomimetic surfaces that display GAGs, either alone or together with other cell ligands, in a background that suppresses non-specific binding. Through the design of the immobilization platform e a streptavidin monolayer serves as a molecular breadboard for the attachment of various biotinylated ligands e and a set of surface-sensitive in situ analysis techniques (including quartz crystal microbalance and spectroscopic ellipsometry), the biomimetic surfaces are tailor made with tight control on biomolecular orientation, surface density and lateral mobility. Analysing the interactions between a selected GAG (heparan sulphate, HS) and the HS-binding chemokine CXCL12a (also called SDF-1a), we demonstrate that these surfaces are versatile for biomolecular and cellular interaction studies. T-lymphocytes are found to adhere specifically to surfaces presenting CXCL12a, both when reversibly bound through HS and when irreversibly immobilized on the inert surface, even in the absence of any bona fide cell adhesion ligand. Moreover, surfaces which present both HS-bound CXCL12a and the intercellular adhesion molecule 1 (ICAM-1) synergistically promote cell adhesion. Our surface biofunctionalization strategy should be broadly applicable for functional studies that require a well-defined supramolecular presentation of GAGs along with other matrix or cell-surface components.

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Viscoelasticity of Thin Biomolecular Films: A Case Study on Nucleoporin Phenylalanine-Glycine Repeats Grafted to a Histidine-Tag Capturing QCM-D Sensor

Cited by 86 publications

References 54 publications

Incorporation of Pentraxin 3 into Hyaluronan Matrices Is Tightly Regulated and Promotes Matrix Cross-linking

Incorporation of Pentraxin 3 into Hyaluronan Matrices Is Tightly Regulated and Promotes Matrix Cross-linking

Sensor Based on Aptamer Folding to Detect Low-Molecular Weight Analytes

Well-defined biomimetic surfaces to characterize glycosaminoglycan-mediated interactions on the molecular, supramolecular and cellular levels

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