Viscosimetric study on compact form of DNA in water—salt solutions containing polyethyleneglycol

Akimenko, N. M.; Dijakowa, E.B.; Evdokimov, Yu. M.; Frisman, E.V.; Varshavsky, Ya.M.

doi:10.1016/0014-5793(73)80513-7

Cited by 16 publications

(6 citation statements)

References 9 publications

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“…The recent viscosimetric study of Akimenko et al [26] also adds weight to the exclusion interaction argument. In this regard it is of interest to compare our results with those of Laurent et al [27] who have demonstrated an exclusion interaction effect of PEO (and dextran) on the Tm for DNA secondary structure at low ionic strength.…”

Section: Resultsmentioning

confidence: 98%

The thermal transition of ‘PSI’ DNA monitored by circular dichroism

Cheng

Mohr

1974

FEBS Letters

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Section: Resultsmentioning

confidence: 98%

The thermal transition of ‘PSI’ DNA monitored by circular dichroism

Cheng

Mohr

1974

FEBS Letters

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“…Accordingly, P = 2πd/Δφ. The electric field at the point where a particular chromophore occurs is a superposition of the field of incident light and the electric fields that are [ [30][31][32][33]). This method of the phase exclusion, depending on two parameters, th molecular mass and its concentration, can be realized as the intramolecular or assembling of these molecules.…”

Section: Some Peculiarities Of the CD Spectra Of Ds Dna Liquid-crystamentioning

confidence: 99%

“…Besides, it has been shown that the mixing of the ds DNA molecules and aqueous-salt solutions of some strongly hydrophilic polymers poly (ethylene glycol), poly (acrylic acid) or poly (vinyl pyrrolidone) leads to the phase exclusion of the DNA (Figure 1). This process has been known since Lerman's experiments (1971) as "ψ-condensation" (psi is the acronym for polymer-salt-induced) [30][31][32][33]). This method of the phase exclusion, depending on two parameters, that is, ds DNA molecular mass and its concentration, can be realized as the intramolecular or intermolecular assembling of these molecules.…”

Section: Introductionmentioning

confidence: 99%

Liquid-Crystalline Dispersions of Double-Stranded DNA

et al. 2019

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In this review, we compare the circular dichroism (CD) spectra of liquid-crystalline dispersion (LCD) particles formed in PEG-containing aqueous-salt solutions with the purpose of determining the packing of ds DNA molecules in these particles. Depending on the osmotic pressure of the solution, the phase exclusion of ds DNA molecules at room temperature results in the formation of LCD particles with the cholesteric or the hexagonal packing of molecules. The heating of dispersion particles with the hexagonal packing of the ds DNA molecules results in a new phase transition, accompanied by an appearance of a new optically active phase of ds DNA molecules. Our results are rationalized by way of a concept of orientationally ordered “quasinematic” layers formed by ds DNA molecules, with a parallel alignment in the hexagonal structure. These layers can adopt a twisted configuration with a temperature increase; and as a result of this process, a new, helicoidal structure of dispersion particle is formed (termed as the “re-entrant” cholesteric phase). To prove the cholesteric pattern of ds DNA molecules in this phase, the “liquid-like” state of the dispersion particles was transformed into its “rigid” counterpart.

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“…Such condensation of a single high-molecularmass (>20 × 10 6 Da) ds DNA molecule is accompanied by formation of a toroid-like DNA particle. This intramolecular process is known since Lerman's experiments (1971) as 'ψ-condensation' (psi is the acronym for polymer-salt-induced) (36)(37)(38)(39).…”

Section: Formation Of the Deoxyribonucleic Acid Liquid-crystalline DImentioning

confidence: 99%

From particles of liquid-crystalline dispersions to rigid deoxyribonucleic acid nanoconstructions

Yevdokimov

2011

Liquid Crystals Today

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Two theoretically feasible strategies to designing the deoxyribonucleic acid (DNA) nanoconstructions are outlined. The main data on the physico-chemical properties of liquid-crystalline dispersions formed by double-stranded nucleic acid (DNA and ribonucleic acid) molecules as a result of their phase exclusion from water-salt solutions are presented and the specific features of these molecules in quasinematic layers of the liquid-crystalline particles are allocated. The basic data obtained in this field have been used as the background for the creation of the DNA nanoconstructions carrying various 'guest' molecules (biologically active compounds or chemical substances). The unique properties of these nanoconstructions determine the possibility of manipulation with these objects, as well as the areas of their practical application.

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Viscosimetric study on compact form of DNA in water—salt solutions containing polyethyleneglycol

Cited by 16 publications

References 9 publications

The thermal transition of ‘PSI’ DNA monitored by circular dichroism

The thermal transition of ‘PSI’ DNA monitored by circular dichroism

Liquid-Crystalline Dispersions of Double-Stranded DNA

From particles of liquid-crystalline dispersions to rigid deoxyribonucleic acid nanoconstructions

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