Viscum album Induces Apoptosis by Regulating STAT3 Signaling Pathway in Breast Cancer Cells

Park, Ye-Rin; Jee, Wona; Park, So-Mi; Kim, Seok Woo; Bae, Hyun Jin; Jung, Ji Hoon; Kim, Hyungsuk; Kim, Sang‐Ki; Chung, Jong Sup; Jang, Hyeung-Jin

doi:10.3390/ijms241511988

Cited by 8 publications

(4 citation statements)

References 35 publications

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“…The new compounds 2f,g and 3c increased the cellular apoptosis in both LoVo and MCF-7 cells in a similar manner, with a higher impact after 48 h of incubation compared with the 24 h experiments, but with little differences between the 5 µM and 50 µM doses. The effect on apoptosis is consistent with the predicted mechanisms of action [40,41]. The diminished impact of the compounds on LoVo cells in contrast to their effects on MCF-7 cells can be explained by the intrinsic resistance of the LoVo cells stemming from inactivating mutations in BAX, a pro-apoptotic member of the Bcl-2 protein family [42].…”

Section: Discussionsupporting

confidence: 81%

Synthesis of 1,3,4-Thiadiazole Derivatives and Their Anticancer Evaluation

Stecoza,

Nitulescu,

Draghici

et al. 2023

IJMS

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Thiadiazole derivatives have garnered significant attention in the field of medicinal chemistry due to their diverse pharmacological activities, including anticancer properties. This article presents the synthesis of a series of thiadiazole derivatives and investigates their chemical characterization and potential anticancer effects on various cell lines. The results of the nuclear magnetic resonance (NMR) analyses confirmed the successful formation of the target compounds. The anticancer potential was evaluated through in silico and in vitro cell-based assays using LoVo and MCF-7 cancer lines. The assays included cell viability, proliferation, apoptosis, and cell cycle analysis to assess the compounds’ effects on cancer cell growth and survival. Daphnia magna was used as an invertebrate model for the toxicity evaluation of the compounds. The results revealed promising anticancer activity for several of the synthesized derivatives, suggesting their potential as lead compounds for further drug development. The novel compound 2g, 5-[2-(benzenesulfonylmethyl)phenyl]-1,3,4-thiadiazol-2-amine, demonstrated good anti-proliferative effects, exhibiting an IC50 value of 2.44 µM against LoVo and 23.29 µM against MCF-7 after a 48-h incubation and little toxic effects in the Daphnia test.

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Section: Discussionsupporting

confidence: 81%

Synthesis of 1,3,4-Thiadiazole Derivatives and Their Anticancer Evaluation

Stecoza,

Nitulescu,

Draghici

et al. 2023

IJMS

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“…The cells were harvested and lysed, and the same protein amounts were calculated using a Bradford assay. The subsequent procedures were the same as those mentioned before [ 14 ].…”

Section: Methodsmentioning

confidence: 99%

“…It has been reported that the inhibition of STAT3 activity could induce apoptosis and autophagy [ 14 , 15 , 16 ]. Apoptosis plays a key role in achieving cellular homeostasis and is characterized by chromatin condensation, cell shrinkage, and DNA fragmentation [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

Euphorbiasteroid Induces Apoptosis as Well as Autophagy through Modulating SHP-1/STAT3 Pathway in Hepatocellular Carcinoma Cells

Kim,

Sethi,

et al. 2023

IJMS

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Euphorbiasteroid (EPBS) has gained attention for its activity against human lung cancer and sarcoma; however, its impact on hepatocellular carcinoma has not yet been elucidated. Here, we investigated the cytotoxic effect of EPBS on human hepatocellular carcinoma (HCC) cells. We found that EPBS induced both apoptosis and autophagy in HCC cells. Additionally, we observed that EPBS treatment suppressed the constitutive as well as the inducible activation of a signal transducer and activator of transcription 3 (STAT3) protein expression. Moreover, EPBS promoted the expression of SHP-1 protein and the production of reactive oxidative stress (ROS). Furthermore, the knockdown of SHP-1 by siRNA transfection reversed the effects of EPBS, which have inductive effects related to apoptosis and autophagy. Therefore, EPBS can potentially function as an anti-cancer agent by inducing apoptosis and autophagy when targeting the SHP-1/STAT3 pathway.

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“…Immunoblotting was performed as described previously [29]. Briefly, ACN-treated cells (1 × 10 4 cells/well) were lysed in a lysis buffer (Cell Signaling Technology, Beverly, MA, USA).…”

Section: Western Blottingmentioning

confidence: 99%

Acetylcorynoline Induces Apoptosis and G2/M Phase Arrest through the c-Myc Signaling Pathway in Colon Cancer Cells

Park,

Jee,

Park

et al. 2023

IJMS

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Colorectal cancer (CRC) is the third most commonly diagnosed cancer worldwide, and despite advances in treatment, survival rates are still low; therefore, the development of novel drugs is imperative. Acetylcorynoline (ACN) is derived from Corydalis ambigua Cham. et Schltdl tubers. The effect of ACN on colon cancer is still unknown. Therefore, we investigated its potential effects. Our data showed that ACN inhibited cell viability and proliferation. Moreover, ACN induced apoptosis and cell cycle arrest by inhibiting cell growth. In the present study, we hypothesized that ACN regulates c-Myc through CNOT2 or MID1IP1. ACN reduced the protein expression of oncogenic genes, decreased c-Myc half-life, and rapidly inhibited the serum stimulation response. Moreover, knockdown of CNOT2 and MID1IP1 with ACN increased apoptosis and further reduced the expression of oncogenes. In addition, ACN exhibited a synergistic effect with low-dose 5-fluorouracil (5-FU) and doxorubicin (Dox). Collectively, our data demonstrate that ACN inhibited c-Myc expression through CNOT2 and MID1IP1, and induced apoptosis. These findings indicate the potential of ACN as a therapeutic agent against colon cancer.

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Viscum album Induces Apoptosis by Regulating STAT3 Signaling Pathway in Breast Cancer Cells

Cited by 8 publications

References 35 publications

Synthesis of 1,3,4-Thiadiazole Derivatives and Their Anticancer Evaluation

Synthesis of 1,3,4-Thiadiazole Derivatives and Their Anticancer Evaluation

Euphorbiasteroid Induces Apoptosis as Well as Autophagy through Modulating SHP-1/STAT3 Pathway in Hepatocellular Carcinoma Cells

Acetylcorynoline Induces Apoptosis and G2/M Phase Arrest through the c-Myc Signaling Pathway in Colon Cancer Cells

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