2017
DOI: 10.33549/physiolres.933370
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Visfatin Is Actively Secreted In Vitro From U-937 Macrophages, but Only Passively Released From 3T3-L1 Adipocytes and HepG2 Hepatocytes

Abstract: Visfatin is a multi-functional molecule that can act intracellularly and extracellularly as an adipokine, cytokine and enzyme. One of the main questions concerning visfatin is the mechanism of its secretion; whether, how and from which cells visfatin is released. The objective of this in vitro study was to observe the active secretion of visfatin from 3T3-L1 preadipocytes and adipocytes, HepG2 hepatocytes, U-937, THP-1 and HL-60 monocytes and macrophages. The amount of visfatin in media and cell lysate was alw… Show more

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Cited by 9 publications
(5 citation statements)
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“…Western blotting was performed and evaluated as described previously (61). Each lysate was resolved by SDS-PAGE (40 g protein/lane, 10% gel) (Mini-PROTEAN Tetra Cell; Bio-Rad).…”
Section: Colocalization Of Nampt With Cell Cycle Markersmentioning
confidence: 99%
“…Western blotting was performed and evaluated as described previously (61). Each lysate was resolved by SDS-PAGE (40 g protein/lane, 10% gel) (Mini-PROTEAN Tetra Cell; Bio-Rad).…”
Section: Colocalization Of Nampt With Cell Cycle Markersmentioning
confidence: 99%
“…The source of eNAMPT and eNAPRT in blood is at present unknown, and it is likely to derive from immune cells. For example, myeloid cells are an important source for eNAMPT, as reported in several manuscripts (10,14,28). For example, myeloid cells are an important source for eNAMPT, as reported in several manuscripts (10,14,28).…”
Section: Discussionmentioning
confidence: 94%
“…For example, myeloid cells are an important source for eNAMPT, as reported in several manuscripts (10,14,28). For example, myeloid cells are an important source for eNAMPT, as reported in several manuscripts (10,14,28). On the other hand, eNAMPT and eNAPRT are also known to be released by the adipose tissue, which could be a further contributor.…”
Section: Discussionmentioning
confidence: 94%
“…Mouse 3T3-L1 preadipocytes were purchased from the American Type Culture Collection and maintained in Dulbecco’s modified Eagle’s medium (DMEM) containing 4.5 g/L d -glucose and 1 mM sodium pyruvate (Gibco, Grand Island, NY, USA), supplemented with 10% foetal bovine serum (Gibco, Grand Island, NY, USA), 4 mM l -glutamine (Gibco, Grand Island, NY, USA), and the mixture of 100 U/mL penicillin and 100 μg/mL streptomycin sulphate (Sigma-Aldrich, St. Louis, MO, USA). The cells were differentiated into mature adipocytes, as described previously [ 17 , 80 ], by the addition of differentiation mixture 0.5 mM 3-isobutyl-1-methylxanthine (IBMX; Sigma-Aldrich, St. Louis, MO, USA), 0.4 µM dexamethasone (Dex; Sigma-Aldrich, St. Louis, MO, USA), 1.7 µM insulin (Ins; figure) and 25 mM HEPES (Sigma-Aldrich, St. Louis, MO, USA). 3T3-L1 cells were differentiated into mature adipocytes for 11 days.…”
Section: Methodsmentioning
confidence: 99%