Visible light crosslinkable chitosan hydrogels for tissue engineering

Hu, Junli; Hou, Yaping; Park, Hyejin; Choi, Bok Hee; Hou, Siying; Chung, Amy S.; Lee, Min

doi:10.1016/j.actbio.2012.01.029

Cited by 192 publications

(185 citation statements)

References 33 publications

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“…In addition, ND safety and biocompatibility have been recently demonstrated in a nonhuman primate study involving systemic administration of NDs to both genders, where no apparent toxicity or impaired organ function was observed (64). Similarly, the safety and biocompatibility of hydrogels allow for their versatile applications in tissue engineering (36)(37)(38)66). Our photo-crosslinked chitosan hydrogel was degradable by lysozyme as confirmed in previous studies (41,42).…”

Section: Discussionsupporting

confidence: 80%

“…Similarly, hydrogels have been extensively used for tissue engineering and drug delivery applications due to their high biocompatibility (36). The application of G at the site of injection allows for the local delivery of bioactive molecules to tissue defects in a minimally invasive manner without the need for any surgical incisions (37)(38)(39). In addition, injectable G facilitates attachment of bioactive agents to the defect structure (40).…”

Section: Significancementioning

confidence: 99%

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Reducing posttreatment relapse in cleft lip palatal expansion using an injectable estrogen–nanodiamond hydrogel

Hong

Song

Lee

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

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Patients with cleft lip and/or palate (CLP), who undergo numerous medical interventions from infancy, can suffer from lifelong debilitation caused by underdeveloped maxillae. Conventional treatment approaches use maxillary expansion techniques to develop normal speech, achieve functional occlusion for nutrition intake, and improve esthetics. However, as patients with CLP congenitally lack bone in the cleft site with diminished capacity for bone formation in the expanded palate, more than 80% of the patient population experiences significant postexpansion relapse. While such relapse has been a long-standing battle in craniofacial care of patients, currently there are no available strategies to address this pervasive problem. Estrogen, 17β-estradiol (E2), is a powerful therapeutic agent that plays a critical role in bone homeostasis. However, E2's clinical application is less appreciated due to several limitations, including its pleiotropic effects and short half-life. Here, we developed a treatment strategy using an injectable system with photo-crosslinkable hydrogel (G) and nanodiamond (ND) technology to facilitate the targeted and sustained delivery of E2 to promote bone formation. In a preclinical expansion/relapse model, this functionalized E2/ ND/G complex substantially reduced postexpansion relapse by nearly threefold through enhancements in sutural remodeling compared with unmodified E2 administration. The E2/ND/G group demonstrated greater bone volume by twofold and higher osteoblast number by threefold, compared with the control group. The E2/ND/G platform maximized the beneficial effects of E2 through its extended release with superior efficacy and safety at the local level. This broadly applicable E2 delivery platform shows promise as an adjuvant therapy in craniofacial care of patients.nanomedicine | cleft palate | craniofacial medicine | nanodiamond | drug delivery

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Section: Discussionsupporting

confidence: 80%

Section: Significancementioning

confidence: 99%

Reducing posttreatment relapse in cleft lip palatal expansion using an injectable estrogen–nanodiamond hydrogel

Hong

Song

Lee

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

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“…Photopolymerizable methacrylated glycol chitosan (MeGC) was prepared as previously described (26,27). ATDC5 cells were harvested and resuspended in 40 l of MeGC solution (2% [wt/vol]) at a density of 1 ϫ 10 7 cells/ml.…”

Section: Methodsmentioning

confidence: 99%

Novel Role for Cyclophilin A in Regulation of Chondrogenic Commitment and Endochondral Ossification

Guo

Shen

Kwak

et al. 2015

Molecular and Cellular Biology

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g Recent studies showed that cyclophilin A (CypA) promotes NF-B/p65 nuclear translocation, resulting in enhanced NF-B activity and altered expression of its target genes, such as the Sox9 transcriptional factor, which plays a critical role in chondrogenic differentiation and endochondral ossification. In this report, we unveil the role of CypA in signal-induced chondrogenic differentiation and endochondral ossification. Expression levels of the chondrogenic differentiation markers and transcriptional regulators Sox9 and Runx2 were all significantly lower in CypA knockdown chondrogenic cells than in wild-type cells, indicating that CypA plays a functional role in chondrogenic differentiation. In vitro differentiation studies using micromass cultures of mouse limb bud cells further supported the conclusion that CypA is needed for chondrogenic differentiation. Newborn CypAdeficient pups double stained with alcian blue and alizarin red exhibited generalized, pronounced skeletal defects, while highresolution micro-computed tomography ( Chondrogenesis is an essential process in vertebrates. It leads to the formation of cartilage growth plates, thereby driving bodily growth while providing structural templates and induction signals for the formation of long bones through endochondral ossification (1). On the other hand, defects in chondrogenesis cause various chondrodysostoses and chondrodysplasias, with such skeletal malformations accounting for a significant proportion of human birth defects that often result in embryonic and perinatal lethality (2). To identify the molecular mechanisms that drive chondrocyte differentiation and impact underlying cartilage diseases, the transcriptional mechanisms governing their cartilage-specific expression have been intensely studied. As a general overview, the chondrocyte differentiation pathway corresponds to a succession of major genetic program switches that are likely controlled by a specific set of transcriptional activators, repressors, and associated factors. While some of these factors play essential roles in determining cell fate and differentiation, other factors are found to be mutated in severe diseases of cartilage and bone malformation (3). The framework of the cartilage matrix is a collagen fiber network comprised primarily of type II collagen (Col2) (encoded by the Col2␣1 gene) and secondarily of type IX collagen (encoded by Col9␣1, Col9␣2, and Col9␣3). Collagen type X (Col10) (encoded by Col10␣1) is also produced in abundance, but exclusively by prehypertrophic and hypertrophic chondrocytes (2).Importantly, chondrocyte differentiation requires Sox family transcription factors in the early stages and Runx2 in the late stages (4, 5). In particular, Sox9 has an essential, nonredundant role in specifying the commitment and differentiation of mesenchymal cells toward the chondrogenic lineage in all developing skeletal elements. While Sox9 is turned on in chondrogenic and osteogenic mesenchymal cells prior to condensation and remains highly expressed in prechondrocytes...

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“…Owing to these groups, photopolymerization can be performed to form chitosan-based hydrogel having highly swollen three-dimensional structure [12]. Photopolymerization technique, which is used to convert a sol-state to a hydrogel form by free radical polymerization, has several advantages over conventional polymerization techniques because it provides i) polymerization in a fast and controllable manner under ambient or physiological conditions ii) spatial and temporal control over polymerization, iii) fast curing rates (from seconds to minutes) at room temperatures [13,14]. Moreover, degradation of sensitive molecules which decompose at a high reaction temperature is avoided since photopolymerization can be conducted at room temperature.…”

Section: Introductionmentioning

confidence: 99%

Synthesis of chitosan-based hydrogel by using photopolymerization technique

Alemdar

2016

AUBTD-A

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In this study, a novel route was developed to fabricate chitosan-based hydrogel by using photopolymerization technique. Firstly, glycidyl methacrylate (GMA) grafted onto the chitosan (CTS) backbone to produce chitosan derivative product having photopolymerizable groups (CTS-g-GMA). Following, CTS-g-GMA and PEGDA are subjected to photopolymerization to form chemically crosslinked hydrogel. The chemical structure of the obtained chitosan-based hydrogel was characterized by Fourier transform infrared (FT-IR) analyses. Thermal behavior of hydrogel was determined by thermal gravimetric analysis (TGA) and differential thermal analysis (DTA). The thermal analyses showed that (CTS-g-GMA)-PEGDA had higher thermal stability than neat chitosan. Water uptake capacities (swelling ratio) of hydrogels obtained by using different UV exposure time were determined gravimetrically. From the swelling test result, it was observed that exposure time has the effect on the water uptake capacity of hydrogels. Additionally, cytotoxicity test for chitosan-based hydrogel was carried out by using L929 fibroblasts. All of the obtained results demonstrated that chitosan-based hydrogel fabricated in this work could be used for biomedical applications in future works.

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Visible light crosslinkable chitosan hydrogels for tissue engineering

Cited by 192 publications

References 33 publications

Reducing posttreatment relapse in cleft lip palatal expansion using an injectable estrogen–nanodiamond hydrogel

Reducing posttreatment relapse in cleft lip palatal expansion using an injectable estrogen–nanodiamond hydrogel

Novel Role for Cyclophilin A in Regulation of Chondrogenic Commitment and Endochondral Ossification

Synthesis of chitosan-based hydrogel by using photopolymerization technique

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