Visible light driven efficient N and Cu co-doped ZnO for photoinactivation of Escherichia coli

Gupta, Rimzhim; Eswar, Neerugatti KrishnaRao; Modak, Jayant M.; Madras, Giridhar

doi:10.1039/c6ra16739j

Cited by 43 publications

(31 citation statements)

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“… 44 − 46 Absolute cell death occurs probably by reactive oxygen species mechanism due to the synergetic effect of both metallic Cu clusters and ZnO in the case of E. coli , as already confirmed by Yu et al 46 We attribute this greater inhibitive effect for E. coli than that for B.cereus to the difference in cell-membrane structures, as supported by Yu et al 46 An additional advantage of Cu 9,18 @ZnO reported here is that the synthetic procedure is very simple and consumes less time and energy. The antibacterial activity could further be activated with the application of visible light, as reported by Gupta et al, 45 wherein the Cu plasmon is expected to play a major role along with ZnO, which will be investigated in the near future.…”

Section: Resultsmentioning

confidence: 96%

“…A recent antibacterial study of E. coli , Staphylococcus aureus , and Streptococcus pyogenes by Bhuyan et al 44 using pure and Cu-doped ZnO NPs showed that Cu-doped ZnO NPs have superior antibacterial action than that of pure ZnO NPs. In another study by Gupta et al, 45 on exposing the E. coli cells to N and Cu co-doped ZnO NPs under visible light irradiation, E. coli becomes inactive. The synergistic antibacterial effect of Cu@T-ZnO nanocomposites for both Gram-negative and Gram-positive bacteria was shown by Yu et al 46 Most importantly, the authors have positively proven them to have significant potential as bactericidal agent, 7 exemplifying a biocidal action of these particles against E. coli ATCC-25922 and Bacillus cereus ATCC-10876.…”

Section: Introductionmentioning

confidence: 97%

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Zinc Oxide-Supported Copper Clusters with High Biocidal Efficacy for Escherichia coli and Bacillus cereus

et al. 2017

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Cu clusters on ZnO have been prepared by a simple low-temperature solid-state reaction from their respective acetate precursors. The formation of metallic Cu along with a small quantity of CuO was influenced by the presence of the zinc acetate precursor. Although there is a lack of formation of any metallic Cu in the absence of zinc acetate, increase in the heating duration helps in the formation of increased metallic Cu. A mechanism for formation of the Cu@ZnO nanocomposite has been suggested. The prepared Cu@ZnO nanocomposite, with metallic Cu, was identified by X-ray diffraction studies followed by confirmation of clusters of the kind Cu9 and Cu18 by transmission electron microscopy and matrix-assisted laser desorption ionization time-of-flight mass spectrometry. The photoelectron spectroscopy is able to clearly distinguish the Cu from CuO, which is very well complimented by electron spin resonance analysis. The morphological feature of ZnO changes from flakes to rods on increasing the duration of heating, as shown by scanning electron microscopy (SEM) analysis. The observed Cu plasmonic band in UV–vis diffuse reflectance gets blue-shifted to 463 nm from its normally observed position of 550–580 nm possibly due to cluster formation and interaction with ZnO, the band gap of the latter getting red-shifted to 3.2–3.0 eV. The antibacterial activity of the synthesized Cu cluster–ZnO nanocomposites was investigated against Escherichia coli ATCC-25922 for Gram-negative and Bacillus cereus ATCC-10876 for Gram-positive bacteria. Tests were performed on a nutrient agar medium and liquid broth supplemented with different concentrations of nanoparticles. SEM analysis of the native and treated Gram-positive and Gram-negative bacteria established a high efficacy of biocide activity in 24 h, with 200 μg/mL of Cu@ZnO nanocomposites.

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Section: Resultsmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 97%

Zinc Oxide-Supported Copper Clusters with High Biocidal Efficacy for Escherichia coli and Bacillus cereus

et al. 2017

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“…In a recent study of Gupta et al, N and Cu co-dopants narrow the bandgap of ZnO NPs effectively [135]. Figure 6a shows the Tauc plots of the Kubelka-Munk function vs photon energy for ZnO NPs, and Cu-doped ZnO NPs with 0.5%, 1.5%, 2.5% and 5.0% Cu, denoting as Cu0.5Z, Cu1.5Z, Cu2.5Z and Cu5.0Z, respectively.…”

Section: Metal/non-metal Co-dopantsmentioning

confidence: 94%

“…Figure 6c reveals that the (N, Cu) co-doped Cu5.0NZ exhibits a higher absorbance than Cu5.0Z in the visible light region. This is due to the synergistic action of both Cu 2+ and N dopants in Cu5.0NZ during the photoexcitation process [135]. Similarly, synergistic effects of both Mn 2+ and N dopants also enhance the absorption of ZnO nanofibers in the visible-light region [136].…”

Section: Metal/non-metal Co-dopantsmentioning

confidence: 99%

Recent Advances in Zinc Oxide Nanostructures with Antimicrobial Activities

Liao

Tjong

2020

IJMS

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This article reviews the recent developments in the synthesis, antibacterial activity, and visible-light photocatalytic bacterial inactivation of nano-zinc oxide. Polycrystalline wurtzite ZnO nanostructures with a hexagonal lattice having different shapes can be synthesized by means of vapor-, liquid-, and solid-phase processing techniques. Among these, ZnO hierarchical nanostructures prepared from the liquid phase route are commonly used for antimicrobial activity. In particular, plant extract-mediated biosynthesis is a single step process for preparing nano-ZnO without using surfactants and toxic chemicals. The phytochemical molecules of natural plant extracts are attractive agents for reducing and stabilizing zinc ions of zinc salt precursors to form green ZnO nanostructures. The peel extracts of certain citrus fruits like grapefruits, lemons and oranges, acting as excellent chelating agents for zinc ions. Furthermore, phytochemicals of the plant extracts capped on ZnO nanomaterials are very effective for killing various bacterial strains, leading to low minimum inhibitory concentration (MIC) values. Bioactive phytocompounds from green ZnO also inhibit hemolysis of Staphylococcus aureus infected red blood cells and inflammatory activity of mammalian immune system. In general, three mechanisms have been adopted to explain bactericidal activity of ZnO nanomaterials, including direct contact killing, reactive oxygen species (ROS) production, and released zinc ion inactivation. These toxic effects lead to the destruction of bacterial membrane, denaturation of enzyme, inhibition of cellular respiration and deoxyribonucleic acid replication, causing leakage of the cytoplasmic content and eventual cell death. Meanwhile, antimicrobial activity of doped and modified ZnO nanomaterials under visible light can be attributed to photogeneration of ROS on their surfaces. Thus particular attention is paid to the design and synthesis of visible light-activated ZnO photocatalysts with antibacterial properties

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“…This method includes sampling of the bacterial solution and its serial dilution to visualize the countable number of colonies on the solid growth media. CFU/mL is counted by the given formula

true CFU / mL = \frac{normaln normalu normalm normalb normale normalr normalo normalf normalc normalo normall normalo normaln normali normale normals (normalf normalo normalr 100 μ normalL normals normala normalm normalp normall normale)}{normald normali normall normalu normalt normali normalo normaln \times normalv normalo normall normalu normalm normale normalo normalf normalt normalh normale normals normala normalm normalp normall normale normalp normall normala normalt normale normald ((), m, L)}

…”

Section: Recent Advancement In the Area Of Disinfection Researchmentioning

confidence: 99%

Bacterial Lysis via Photocatalysis ‐ A Critical Mechanistic Review

Gupta

Modak

2020

ChemCatChem

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This Review discusses one of the most important area of photocatalytic reactions. In current research scenario, photocatalysis is well utilized strategy for a wide variety of applications including organic/ inorganic decontamination, solar fuel generation, microbial disinfection etc. This Review touches upon the mechanistic aspect of microbial disinfection, the growth in the research, issues to be addressed in future to make it a sustainable technology for this application. This Review discusses the fundamental understanding of photocatalysis, important strategies to tune their optical properties to enhance the spectral range of absorption in visible and near infrared wavelength region. Various photocatalysis reports these days don't focus on the stability, reusability and recovery of the photocatalysts. However, these issues are very important to deal with for large scale viability of the process. Therefore, we have tried to address these issues along with the detailed experimental information and eliminate the misconceptions about bacterial disinfection. The details provided in this Review would help the researchers working in this area to a large extent and would open various questions for them to answer.

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Visible light driven efficient N and Cu co-doped ZnO for photoinactivation of Escherichia coli

Abstract: N and Cu co-doped ZnO shows outstanding antibacterial activity for E. coli inactivation under visible light.

Cited by 43 publications

References 64 publications

Zinc Oxide-Supported Copper Clusters with High Biocidal Efficacy for Escherichia coli and Bacillus cereus

Zinc Oxide-Supported Copper Clusters with High Biocidal Efficacy for Escherichia coli and Bacillus cereus

Recent Advances in Zinc Oxide Nanostructures with Antimicrobial Activities

Bacterial Lysis via Photocatalysis ‐ A Critical Mechanistic Review

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