2009
DOI: 10.1021/ja806489y
|View full text |Cite
|
Sign up to set email alerts
|

Visible Light Excitable Zn2+ Fluorescent Sensor Derived from an Intramolecular Charge Transfer Fluorophore and Its in Vitro and in Vivo Application

Abstract: The UV- and sensor-induced interferences to living systems pose a barrier for in vivo Zn(2+) imaging. In this work, an intramolecular charge transfer (ICT) fluorophore of smaller aromatic plane, 4-amino-7-nitro-2,1,3-benzoxadiazole, was adopted to construct visible light excited fluorescent Zn(2+) sensor, NBD-TPEA. This sensor demonstrates a visible ICT absorption band, a large Stokes shift, and biocompatibility. It emits weakly (Phi = 0.003) without pH dependence at pH 7.1-10.1, and the lambda(ex) and lambda(… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1
1

Citation Types

6
174
1
1

Year Published

2009
2009
2016
2016

Publication Types

Select...
7
2

Relationship

0
9

Authors

Journals

citations
Cited by 413 publications
(182 citation statements)
references
References 53 publications
6
174
1
1
Order By: Relevance
“…NBD-TPEA was employed as a visible light-excitable Zn 2þ fluorescent sensor with low background fluorescence for intracellular Zn 2þ imaging, because the staining displays high quality. 32) A working solution of NBD-TPEA (10 mM) for cell staining was prepared from a 1 mM NBD-TPEA stock solution in DMSO by diluting with PBS. Cells seeded on 20-mmdiameter glass-bottom dishes were incubated as described before.…”
Section: Methodsmentioning
confidence: 99%
“…NBD-TPEA was employed as a visible light-excitable Zn 2þ fluorescent sensor with low background fluorescence for intracellular Zn 2þ imaging, because the staining displays high quality. 32) A working solution of NBD-TPEA (10 mM) for cell staining was prepared from a 1 mM NBD-TPEA stock solution in DMSO by diluting with PBS. Cells seeded on 20-mmdiameter glass-bottom dishes were incubated as described before.…”
Section: Methodsmentioning
confidence: 99%
“…The fluorescence properties of BODIPY were tunable by simple substitution at various positions of the difluoroboron dipyrromethene core [25][26][27]. DPA and its derivatives DPEA and TPEA, are well-known receptors for heavy-metal ions [28][29][30]. The DPA derivatives combine high affinities for a variety of heavy-metal ions with the capacity to perturb the fluorescence properties of BODIPY, making them the most promising candidates for the design of fluorometric sensor arrays for heavy-metal ions.…”
Section: Resultsmentioning
confidence: 99%
“…[13,16] However, compound 1 is the first Zn 2 + -sensitive off-on fluorescent sensor molecule based on the ICT mechanism on a 4-amino-1,8-naphthalimide platform, though a similar ICT working principle was reported with 7-nitrobenz-2-oxa-1,3-diazole (NBD). [17] Our design strategy should be applicable to a range of off-on fluorescent sensor molecules for Zn 2 + and other molecules of interest in biological applications.…”
Section: Discussionmentioning
confidence: 99%