Visible Light Modulates the Expression of Cancer-Retina Antigens

Bazhin, Alexandr V.; Schadendorf, Dirk; Owen, Robert W.; Zernii, Evgeni Yu; Philippov, Pavel P.; Eichmüller, Stefan B.

doi:10.1158/1541-7786.mcr-07-0140

Cited by 12 publications

(11 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Such expression of cancer-retina antigens could generate an autoimmune response [8], which in turn might lead to development of a paraneoplastic neurological syndrome, melanoma-associated retinopathy [9]. Furthermore, we demonstrated that expression of cancer-retina antigens can be modulated by light as it proceeds in photoreceptor cells [10]. Whereas the biochemistry and physiology of visual transduction have been extensively studied, the role of the photoreceptor proteins in melanoma cells still remains to be resolved.…”

Section: Introductionmentioning

confidence: 83%

See 1 more Smart Citation

RETRACTED ARTICLE: cGMP-phosphodiesterase 6, transducin and Wnt5a/Frizzled-2-signaling control cGMP and Ca2+ homeostasis in melanoma cells

Bazhin

Tambor

Dikov

et al. 2009

Cell. Mol. Life Sci.

Self Cite

View full text Add to dashboard Cite

Malignant melanoma is one of the most aggressive human neoplasms which develop from the malignant transformation of normal epithelial melanocytes and share the lineage with retinal cells. cGMP-phosphodiesterase 6 (PDE6) is one of the cancer-retina antigens newly identified in melanoma cells. Normally, PDE6 hydrolyzes the photoreceptor second messenger cGMP allowing the visual signal transduction in photoreceptor cells. cGMP also play an important signaling role in stimulating melanogenesis in human melanocytes. Here, we present evidence that PDE6 is a key enzyme regulating the cGMP metabolism in melanoma cells. Decrease in intracellular cGMP leads to calcium accumulation in melanoma cells. In these cells, cGMP-phosphodiesterase 6 can be activated by another cancer-retina antigen, transducin, through Wnt5a-Frizzled-2 cascade, which leads to a lowering of cGMP and an increase in intracellular calcium mobilization. Thus, the aberrant expression of PDE6 may control cGMP metabolism and calcium homeostasis in melanoma cells.

show abstract

Section: Introductionmentioning

confidence: 83%

“…Western blot analysis and ELISA were performed as described elsewhere [10]. cGMP measurement cGMP concentration was measured with the HitHunter cGMP Assay (Amersham Bioscience Europe, Freiburg, Germany) as described by the manufacturer.…”

Section: Protein Analysismentioning

confidence: 99%

RETRACTED ARTICLE: cGMP-phosphodiesterase 6, transducin and Wnt5a/Frizzled-2-signaling control cGMP and Ca2+ homeostasis in melanoma cells

Bazhin

Tambor

Dikov

et al. 2009

Cell. Mol. Life Sci.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Cell lines were cultivated at 378C and 5% CO 2 . RNA isolation and analysis, RT-PCR analysis, and real-time RT-PCR were performed as described in Bazhin et al [2008].…”

Section: Cell Culturesmentioning

confidence: 99%

Expression and activity of alcohol and aldehyde dehydrogenases in melanoma cells and in melanocytes

Amann¹,

Hofmann²,

Freudenberger³

et al. 2012

J of Cellular Biochemistry

Self Cite

View full text Add to dashboard Cite

Disturbances in vitamin A metabolism are an important attribute of some cancer cells. Most evidence point that these disturbances lead to decreasing of the retinoic acid concentration in tumor cells. Up to now, in benign and malignant skin cells the features of vitamin A metabolism with its participating enzymes are not entirely understood. Alcohol and aldehyde dehydrogenases (ALDH) are involved in the retinol metabolism, oxidizing retinol, and retinal in retinoic acid or reducing retinal in retinol. In this work we investigated the expression and enzymatic activity of alcohol and ALDH in melanoma cells compared to their benign counterparts. We demonstrated that melanoma cell lines and melanocytes despite similar pattern of the enzyme expression, show different general ALDH activity. Retinal, the substrate of ALDH, could stimulate the ALDH activity through up-regulation of retinaldehyde dehydrogenase 1 and aldehyde dehydrogenase 6. Furthermore, we found that retinoids regulate alcohol dehydrogenase activity, probably via effects on alcohol dehydrogenase expression at the post-transcriptional level. We suggest that melanoma cells in contrast to melanocytes should favor the retinal reduction over its oxidation. The decreasing cellular amount of the precursor molecules of retinoic acid could result in a changed gene regulation in melanoma cells.

show abstract

“…RT-PCR primers for murine PKGI, PKGII, β-actin, and GAPDH were purchased from Qiagen (Germany). Conventional RT-PCR analysis of PKGI and PKGII was performed as described elsewhere (Bazhin et al 2008). Briefly, 1 μg of the total RNA from cell lines or tissues was reversetranscribed by using the 1st strand complementary DNA (cDNA) synthesis kit (Roche Diagnostics, Germany) at 42°C for 50 min as described by the manufacturer.…”

Section: Rna Isolation Conventional and Real-time Rt-pcr Analysesmentioning

confidence: 99%

Anti-tumor properties of the cGMP/protein kinase G inhibitor DT3 in pancreatic adenocarcinoma

Soltek

Karakhanova

Golovastova

et al. 2015

Naunyn-Schmiedeberg's Arch Pharmacol

Self Cite

View full text Add to dashboard Cite

Pancreatic ductal adenocarcinoma (PDAC) is one of the deadliest cancers in the world. Therefore, new therapeutic options are urgently needed to improve the survival of PDAC patients. Protein kinase G (PKG) conducts the interlude of cGMP signaling which is important for healthy as well as for cancer cells. DT3 is a specific inhibitor of PKG, and it has been shown to possess an anti-tumor cytotoxic activity in vitro. The main aim of this work was to investigate anti-tumor effects of DT3 upon PDAC in vivo.Expression of PKG was assessed with real-time PCR analysis in the normal and tumor pancreatic cells. In vitro cell viability, proliferation, apoptosis, necrosis, migration, and invasion of the murine PDAC cell line Panc02 were assessed after DT3 treatment. In vivo anti-tumor effects of DT3 were investigated in the murine Panc02 orthotopic model of PDAC. Western blot analysis was used to determine the phosphorylation state of the proteins of interest.Functional PKGI is preferentially expressed in PDAC cells. DT3 was capable to reduce viability, proliferation, and migration of murine PDAC cells in vitro. At the same time, DT3 treatment did not change the viability of normal epithelial cells of murine liver. In vivo, DT3 treatment reduced the tumor volume and metastases in PDAC-bearing mice, but it was ineffective to prolong the survival of the tumor-bearing animals. In addition, DT3 treatment decreased phosphorylation of GSK-3, P38, and CREB in murine PDAC.Inhibition of PKG could be a potential therapeutic strategy for PDAC treatment which should be carefully validated in future pre-clinical studies.

show abstract

Visible Light Modulates the Expression of Cancer-Retina Antigens

Cited by 12 publications

References 36 publications

RETRACTED ARTICLE: cGMP-phosphodiesterase 6, transducin and Wnt5a/Frizzled-2-signaling control cGMP and Ca2+ homeostasis in melanoma cells

RETRACTED ARTICLE: cGMP-phosphodiesterase 6, transducin and Wnt5a/Frizzled-2-signaling control cGMP and Ca2+ homeostasis in melanoma cells

Expression and activity of alcohol and aldehyde dehydrogenases in melanoma cells and in melanocytes

Anti-tumor properties of the cGMP/protein kinase G inhibitor DT3 in pancreatic adenocarcinoma

Contact Info

Product

Resources

About