Blur adaptation is the improvement of visual and perceptual performance with time following viewing of a target blurred by some combination of defocus, astigmatism and higher-order aberrations. Blurred images can be produced by two different methods -source and observer. In the source method, image convolution between the point spread function of intended aberration and object produces blurred images on a display screen. In the observer method, the blurred images are produced optically such as by deformable mirrors. Previous studies on higher-order aberration blur adaptation were restricted to the source method. This study compared blur adaptation between source and observer methods for combinations of defocus and higher-order aberrations. It is important to compare blur adaptation with source and observer methods as the first one blurred the stimulus not the surroundings while the second one blurred both the stimulus and surroundings. On the basis that the observer method has phase information lacking in the source method, the main hypothesis of the study was that blur adaption would be greater with the observer method than with the source method.A custom built adaptive optics system had three channels -an illumination channel, an adaptive optics control channel with deformable mirror and Hartmann-Shack wavefront sensor, and a psychophysical channel with a display. Blurred images of a natural scene (adapting images) and tumbling Es (testing images) were generated for +2 D, −2 D and 0 D defocus with and without higher order aberrations of the eye. Five participants performed a four alternative forced choice procedure with the method of constant stimuli to obtain visual acuity. They adapted to the natural scene for 1 minute, followed by testing images for 1 second, and refreshing of the adapting image for 3 seconds.In the source method, mean ± SEM of visual acuities were 0.42 ± 0.08 logMAR without blur adaptation and 0.39 ± 0.09 logMAR with blur adaptation, giving a small, but statistically significant improvement of 0.03 ± 0.01 logMAR (p = 0.03) with blur adaptation. In the observer method, mean ± SEM of visual acuities were 0.16 ± 0.12 logMAR without blur adaptation and 0.15 ± 0.13 logMAR with blur adaptation, giving a non-significant difference of 0.01 ± 0.03 logMAR (p = 0.77). The mean ± SEM of difference in visual acuity improvement after blur adaptation between source and observer method was not significant at 0.02 ± 0.02 logMAR (p = 0.37). Visual acuities were always better with the observer method than with the source method.Blur adaptation found with both source and observer methods and the comparison of blur adaptation between source and observer methods was not significant. A longer adapting period might be necessary to achieve significant visual acuity improvement following blur adaptation. Replacement of the method of constant stimuli with an adaptive psychophysical procedure such as QUEST (Quick Estimate by iii Sequential Testing) or PEST (Parametric Estimate by Sequential Testing) will shor...