Visual and Electronic Evaluations of Detached Strawberry Leaves Inoculated with<i>Colletotrichum</i>Species

Miller-Butler, Melinda A.; Curry, Kenneth J.; Kreiser, Brian R.; Smith, Barbara J.

doi:10.1080/15538362.2012.697033

Cited by 3 publications

(4 citation statements)

References 6 publications

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“…The visual 0 to 5 disease rating scale used was sufficient to separate susceptible from resistant strawberry leaves. The degree of agreement between the two methods (visual ratings and image analysis) used in this study was determined to be positively related (r p = 0.79) and supports a preliminary study (Miller-Butler et al, 2013) that also found a strong correlation between image analysis and visual disease ratings.…”

Section: Discussionsupporting

confidence: 86%

“…of the lesions before analysis improved their visibility (Miller-Butler et al, 2013). Initial screening of large strawberry seedling populations for anthracnose resistance can be accomplished by field screening; however, if the lack of natural inoculum or the possible introduction of new strains of a pathogen prevents field screening, then preliminary screening using this detached leaf assay and visual disease assessment provides a reliable alternative.…”

Section: Discussionmentioning

confidence: 99%

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Evaluation of Detached Strawberry Leaves for Anthracnose Disease Severity Using Image Analysis and Visual Ratings

Miller-Butler¹,

Smith²,

Curry³

et al. 2019

horts

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Inoculation of detached strawberry leaves with Colletotrichum species may provide an accurate, rapid, nondestructive method of identifying anthracnose-resistant germplasm. The purpose of this study was to statistically compare two methods (visual and image analysis) of evaluating disease severity of strawberry germplasm screened for anthracnose resistance. Detached leaves of 77 susceptible and resistant strawberry clones were inoculated with one Colletotrichum gloeosporioides (Penz.) Penz. & Sacc. and two C. fragariae A. N. Brooks isolates. Anthracnose disease symptoms on each leaf were assessed quantitatively via computer-based image analysis to determine percentage lesion area and qualitatively by two independent raters using a visual disease severity rating scale (0 = no symptoms to 5 = entire leaf dead). The two visual raters’ average disease severity ratings (n = 3413) were in substantial agreement with a weighted Cohen’s kappa coefficient (k) of 0.80 [95% confidence interval (CI) 0.79–0.82]. There was a strong positive correlation between percent lesion area determined by image analysis and the visual disease scores of the two raters (rp = 0.79). Image analysis provided a precise measurement of percent lesion area of infected leaves while visual assessment provided more rapid results. Our results indicate that detached leaf inoculations can be used as a rapid preliminary screen to separate anthracnose-susceptible from -resistant germplasm in large populations within breeding programs. It also may be used for assessing the resistance/susceptibility of parental breeding lines to various Colletotrichum species and isolates, for mapping germplasm for resistance genes, and in pesticide development studies.

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Section: Discussionsupporting

confidence: 86%

Section: Discussionmentioning

confidence: 99%

Evaluation of Detached Strawberry Leaves for Anthracnose Disease Severity Using Image Analysis and Visual Ratings

Miller-Butler¹,

Smith²,

Curry³

et al. 2019

horts

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“…Preliminary research indicated that anthracnose-resistant germplasm could be distinguished from susceptible germplasm using inoculated detached strawberry leaves and that a 0 to 5 ordinal scale was sufficient for rating the disease severity (Miller-Butler et al 2013). Our current research compared the disease severity ratings following inoculation of whole plants (benchmark), a single leaf from each of the whole plants, and detached leaves, and found that all three assays were able to distinguish significant differences among the resistance categories (Table 2).…”

Section: Discussionmentioning

confidence: 77%

“…Detached leaf inoculation assays are being used to determine plant germplasm resistance to disease, such as for tomato, Solanum lycopersicum L., germplasm resistant to late blight (Foolad et al 2015), apple genotypes being tested for resistance to Alternaria blotch (Abe et al 2010), and American chestnut, Castanea dentata, germplasm resistant to chestnut blight (Newhouse et al 2014). Research on the development of a detached strawberry leaf inoculation protocol has been ongoing at the USDA-ARS in Poplarville, MS (Miller-Butler et al 2013). Our goal was to develop a reliable anthracnose screening protocol using a detached leaf that would reduce the time between inoculation and disease assessment.…”

mentioning

confidence: 99%

Comparison of Whole Plant and Detached Leaf Screening Techniques for Identifying Anthracnose Resistance in Strawberry Plants

et al. 2018

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Anthracnose is a destructive disease of strawberry caused by several Colletotrichum species including C. acutatum, C. fragariae, and C. gloeosporioides. Identification of anthracnose resistant strawberry germplasm has commonly relied on inoculation of whole plants with isolates of these pathogens. In this study, whole plants and detached leaves from 81 germplasm lines were inoculated with a conidial suspension of isolates of C. acutatum, C. fragariae, and C. gloeosporioides, incubated in the dark at 30°C, 100% relative humidity, for 48 h, and assessed for disease severity based on symptoms on inoculated petioles and leaves. The correlation between the disease severity ratings of the whole plants rated 30 days after inoculation and detached leaves rated 5 days after inoculation was determined. Based on leaf symptoms and petiole lesions, the association between the whole plant leaf disease severity rating (DSR) and detached leaf DSR was positive (r = 0.70), and the association between the whole plant DSR and the detached leaf DSR was also positive (r = 0.66). Whole plant and detached leaf DSRs were used to assign each germplasm line to a resistance category, and a posthoc Tukey's test showed that the whole plant DSR means and the detached leaf DSR means for each resistance category differed significantly at p < 0.05. This research was used to develop a strawberry detached leaf assay which can reliably and quickly determine the degree of resistance of strawberry germplasm to anthracnose.

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A new approach for strawberry disease control

Takeda¹,

Janisiewicz²,

Smith³

et al. 2019

European.J.Hortic.Sci

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Organic farming is one of the fastest growing segments of U.S. agriculture. As consumer interest continues to gather momentum, many U.S. producers, manufacturers, distributors, and retailers are specializing in growing, processing, and marketing an ever-widening array of organic agricultural and food products. This report summarizes growth patterns in the U.S. organic sector in recent years, by market category, and describes various research, regulatory, and other ongoing programs on organic agriculture in the U.S. Department of Agriculture.

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Visual and Electronic Evaluations of Detached Strawberry Leaves Inoculated withColletotrichumSpecies

Cited by 3 publications

References 6 publications

Evaluation of Detached Strawberry Leaves for Anthracnose Disease Severity Using Image Analysis and Visual Ratings

Evaluation of Detached Strawberry Leaves for Anthracnose Disease Severity Using Image Analysis and Visual Ratings

Comparison of Whole Plant and Detached Leaf Screening Techniques for Identifying Anthracnose Resistance in Strawberry Plants

A new approach for strawberry disease control

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