Visual and real-time imaging focusing for highly sensitive laser-induced fluorescence detection at yoctomole levels in nanocapillaries

Zhang, Wenmei; Liu, Lei; Zhang, Qi; Zhang, Dongtang; Hu, Qin; Wang, Yanan; Wang, Xiayan; Pu, Qiaosheng; Guo, Guangsheng

doi:10.1039/c9cc09594b

Cited by 14 publications

(8 citation statements)

References 37 publications

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“…Based on our previous work on narrow-bore capillary chromatography, 35–41 we developed a single-cell MS analysis method using a novel ILCEI technique. This analysis method aims to realize high-throughput single-cell online dispersion, dilution-free MS injection, and high-sensitivity MS detection.…”

Section: Resultsmentioning

confidence: 99%

Intact living-cell electrolaunching ionization mass spectrometry for single-cell metabolomics

et al. 2022

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Section: Resultsmentioning

confidence: 99%

Intact living-cell electrolaunching ionization mass spectrometry for single-cell metabolomics

et al. 2022

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“…Combined with qualitative scattering imaging, it is possible to realize the qualitative and quantitative analyses of mixed label-free high-density nanoplastics as small as a few nm in the subregions of living cells in the future. In addition, the new injection-transfer method is compatible with a variety of living single-cell sampling techniques such as dielectrophoresis, SPME, and electrowetting. − It is also suitable for many online detectors based on laser-induced fluorescence, electrochemistry, and contactless conductivity measurement. , This method features less invasive sampling, high sample utilization rate, and high ability to separate substances, and thus, it has great potential in studying subcellular metabolism or proteomics . The limitation of this work is that the system requires complex devices.…”

Section: Discussionmentioning

confidence: 99%

Quantitative Analysis of Nanoplastics in Single Cells by Subcellular Chromatography

et al. 2023

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The accumulation and spatial distribution of intracellular nanoplastic particles provide useful information about their spatiotemporal toxicological effects mediated by the physicochemical parameters of nanoplastics in living cells. In this study, a sample injection-transfer method was designed with an accuracy of up to femtoliters to attoliters to match the volume required for ultranarrow-bore open-tubular liquid chromatography. The separation and concentration quantification of mixed polystyrenes in different regions in living cells were achieved by directly transferring picoliter/femtoliter volumes of intracellular cytoplasm to an ultranarrow-bore open-tubular chromatographic column. The measurement of pollutant concentration in different areas of a small-volume target (single cell) was realized. This method is expected to be used in the qualitative and quantitative analyses of complex, mixed, and label-free nanoplastics (a few nm in size) in the subregions of living cells.

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“…The samples were prepared by drop-casting an appropriate amount of ethanol dispersion of the products on silicon wafers for XRD measurement, or carbon-coated copper grids for TEM and SEM imaging, followed by drying in a fume hood and at room temperature. Laser scattering of microfluidic flow was recorded using the home-built microscopic system detailed in our previous work [ 21 ].…”

Section: Methodsmentioning

confidence: 99%

Spontaneous Phase Segregation Enabling Clogging Aversion in Continuous Flow Microfluidic Synthesis of Nanocrystals Supported on Reduced Graphene Oxide

et al. 2022

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Eliminating clogging in capillary tube reactors is critical but challenging for enabling continuous-flow microfluidic synthesis of nanoparticles. Creating immiscible segments in a microfluidic flow is a promising approach to maintaining a continuous flow in the microfluidic channel because the segments with low surface energy do not adsorb onto the internal wall of the microchannel. Herein we report the spontaneous self-agglomeration of reduced graphene oxide (rGO) nanosheets in polyol flow, which arises because the reduction of graphene oxide (GO) nanosheets by hot polyol changes the nanosheets from hydrophilic to hydrophobic. The agglomerated rGO nanosheets form immiscible solid segments in the polyol flow, realizing the liquid–solid segmented flow to enable clogging aversion in continuous-flow microfluidic synthesis. Simultaneous reduction of precursor species in hot polyol deposits nanocrystals uniformly dispersed on the rGO nanosheets even without surfactant. Cuprous oxide (Cu2O) nanocubes of varying edge lengths and ultrafine metal nanoparticles of platinum (Pt) and palladium (Pd) dispersed on rGO nanosheets have been continuously synthesized using the liquid–solid segmented flow microfluidic method, shedding light on the promise of microfluidic reactors in synthesizing functional nanomaterials.

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Visual and real-time imaging focusing for highly sensitive laser-induced fluorescence detection at yoctomole levels in nanocapillaries

Abstract: We developed a highly sensitive laser-induced fluorescence detection system, involving visual and real-time imaging focusing instead of the use of fluorescent reagents, for the detection of analytes in nanocapillaries.

Cited by 14 publications

References 37 publications

Intact living-cell electrolaunching ionization mass spectrometry for single-cell metabolomics

Intact living-cell electrolaunching ionization mass spectrometry for single-cell metabolomics

Quantitative Analysis of Nanoplastics in Single Cells by Subcellular Chromatography

Spontaneous Phase Segregation Enabling Clogging Aversion in Continuous Flow Microfluidic Synthesis of Nanocrystals Supported on Reduced Graphene Oxide

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