Visual-Based Impedance Control of Out-of-Plane Cell Injection Systems

Huang, Haibo; Sun, Dong; Mills, James K.; Li, Wen J.; Cheng, Shuk Han

doi:10.1109/tase.2008.2010013

Cited by 97 publications

(5 citation statements)

References 22 publications

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“…Manual methods require >1 min per cell (not including locating or retrieving the sample) and suffer from high variability (standard deviation of ±30% of original total volume removed) and low viability (∼40%) . While efforts have been made to use robotically controlled micropipettes and/or computer vision to automate and enhance processes such as enucleation, − microfluidic methods offer a unique advantage in facilitating high precision and high throughput enucleation procedures.…”

Section: Sectioningmentioning

confidence: 99%

Microfluidic Surgery in Single Cells and Multicellular Systems

et al. 2022

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Microscale surgery on single cells and small organisms has enabled major advances in fundamental biology and in engineering biological systems. Examples of applications range from wound healing and regeneration studies to the generation of hybridoma to produce monoclonal antibodies. Even today, these surgical operations are often performed manually, but they are labor intensive and lack reproducibility. Microfluidics has emerged as a powerful technology to control and manipulate cells and multicellular systems at the micro- and nanoscale with high precision. Here, we review the physical and chemical mechanisms of microscale surgery and the corresponding design principles, applications, and implementations in microfluidic systems. We consider four types of surgical operations: (1) sectioning, which splits a biological entity into multiple parts, (2) ablation, which destroys part of an entity, (3) biopsy, which extracts materials from within a living cell, and (4) fusion, which joins multiple entities into one. For each type of surgery, we summarize the motivating applications and the microfluidic devices developed. Throughout this review, we highlight existing challenges and opportunities. We hope that this review will inspire scientists and engineers to continue to explore and improve microfluidic surgical methods.

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Section: Sectioningmentioning

confidence: 99%

Microfluidic Surgery in Single Cells and Multicellular Systems

et al. 2022

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“…D. Sun et al [32] and Y. Sun et al [66] proposed a cell membrane point load model to determine the relationship between the applied force and cell deformation.…”

Section: Characterization Of Cell Modelsmentioning

confidence: 99%

“…However, most injection strategies still rely on a holding pipette to immobilize a single cell, which greatly limits the efficiency of the cell injection process. Some automated suspended cell injection strategies [30,32,33] are complicated to use and involve a time-consuming injection process.…”

Section: Introductionmentioning

confidence: 99%

A Review of Automated Microinjection of Zebrafish Embryos

et al. 2018

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Cell microinjection is a technique of precise delivery of substances into cells and is widely used for studying cell transfection, signaling pathways, and organelle functions. Microinjection of the embryos of zebrafish, the third most important animal model, has become a very useful technique in bioscience. However, factors such as the small cell size, high cell deformation tendency, and transparent zebrafish embryo membrane make the microinjection process difficult. Furthermore, this process has strict, specific requirements, such as chorion softening, avoiding contacting the first polar body, and high-precision detection. Therefore, highly accurate control and detection platforms are critical for achieving the automated microinjection of zebrafish embryos. This article reviews the latest technologies and methods used in the automated microinjection of zebrafish embryos and provides a detailed description of the current developments and applications of robotic microinjection systems. The review covers key areas related to automated embryo injection, including cell searching and location, cell position and posture adjustment, microscopic visual servoing control, sensors, actuators, puncturing mechanisms, and microinjection.

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“…The cell injection process requires high precision movement of the micro-pipette to accurately perform the delicate tasks of puncture, penetration and deposition and thus the injection force is the key factor in the survivability of the injected cells [12]. Accurate measurement and control of the injection force is of vital importance because even a minute excessive force can result in missing the desired deposition point [6] and thus lead to the failure of the injection task [10].…”

Section: Introductionmentioning

confidence: 99%

Towards Probabilistic Formal Modeling of Robotic Cell Injection Systems

Sardar

Hasan

2017

Electron. Proc. Theor. Comput. Sci.

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Cell injection is a technique in the domain of biological cell micro-manipulation for the delivery of small volumes of samples into the suspended or adherent cells. It has been widely applied in various areas, such as gene injection, in-vitro fertilization (IVF), intracytoplasmic sperm injection (ISCI) and drug development. However, the existing manual and semi-automated cell injection systems require lengthy training and suffer from high probability of contamination and low success rate. In the recently introduced fully automated cell injection systems, the injection force plays a vital role in the success of the process since even a tiny excessive force can destroy the membrane or tissue of the biological cell. Traditionally, the force control algorithms are analyzed using simulation, which is inherently non-exhaustive and incomplete in terms of detecting system failures. Moreover, the uncertainties in the system are generally ignored in the analysis. To overcome these limitations, we present a formal analysis methodology based on probabilistic model checking to analyze a robotic cell injection system utilizing the impedance force control algorithm. The proposed methodology, developed using the PRISM model checker, allowed to find a discrepancy in the algorithm, which was not found by any of the previous analysis using the traditional methods.

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Visual-Based Impedance Control of Out-of-Plane Cell Injection Systems

Cited by 97 publications

References 22 publications

Microfluidic Surgery in Single Cells and Multicellular Systems

Microfluidic Surgery in Single Cells and Multicellular Systems

A Review of Automated Microinjection of Zebrafish Embryos

Towards Probabilistic Formal Modeling of Robotic Cell Injection Systems

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