Visual Behavior in Zebrafish

Fleisch, Valerie C.; Neuhauss, Stephan C.F.

doi:10.1089/zeb.2006.3.191

Cited by 170 publications

(113 citation statements)

References 66 publications

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“…[17][18][19][20][21][22][23][24][25][26][27] The lens in zebrafish eyes is spheroid and not ellipsoid as compared with the human eye, and as a consequence, the zebrafish eye has a much lower volume of vitreous compared with the human eye ( Figure 1a). …”

Section: Eye Structurementioning

confidence: 99%

“…As zebrafish use vision to protect themselves against predators and depend on light to search their food, their visual system develops rapidly. 23 The retinal structure starts to develop from 32 hpf and continues to develop extraordinarily fast within 5 days post fertilization (dpf). 17 By then, the zebrafish retina becomes functional.…”

Section: Eye Structurementioning

confidence: 99%

“…In contrast, the human eye lacks the UV-sensitive cones and therefore has only trichromatic vision. 23 Unlike cones that have four types of opsins as their photopigment, the rods consist of only a single type and contain rhodopsin as their photopigment. 23 During the development of the zebrafish retina, the SSCs become distinct at 5-6 dpf while LSCs appear at 7-8 dpf and the DCs at 10-12 dpf.…”

Section: Eye Structurementioning

confidence: 99%

“…23 Unlike cones that have four types of opsins as their photopigment, the rods consist of only a single type and contain rhodopsin as their photopigment. 23 During the development of the zebrafish retina, the SSCs become distinct at 5-6 dpf while LSCs appear at 7-8 dpf and the DCs at 10-12 dpf. However, all of the visual pigments are expressed between 50 and 55 hpf, so the various cone types are presumably there at 50-55 hpf but not recognizable.…”

Section: Eye Structurementioning

confidence: 99%

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Zebrafish—on the move towards ophthalmological research

Chhetri

Jacobson

Gueven

2014

Eye

138

114

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Millions of people are affected by visual impairment and blindness globally, and the prevalence of vision loss is likely to increase as we are living longer. However, many ocular diseases remain poorly controlled due to lack of proper understanding of the pathogenesis and the corresponding lack of effective therapies. Consequently, there is a major need for animal models that closely mirror the human eye pathology and at the same time allow higher-throughput drug screening approaches. In this context, zebrafish as an animal model organism not only address these needs but can in many respects reflect the human situation better than the current rodent models. Over the past decade, zebrafish have become an established model to study a variety of human diseases and are more recently becoming a valuable tool for the study of human ophthalmological disorders. Many human ocular diseases such as cataract, glaucoma, diabetic retinopathy, and age-related macular degeneration have already been modelled in zebrafish. In addition, zebrafish have become an attractive model for pre-clinical drug toxicity testing and are now increasingly used by scientists worldwide for the discovery of novel treatment approaches. This review presents the advantages and uses of zebrafish for ophthalmological research.

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Section: Eye Structurementioning

confidence: 99%

Section: Eye Structurementioning

confidence: 99%

Section: Eye Structurementioning

confidence: 99%

Section: Eye Structurementioning

confidence: 99%

See 2 more Smart Citations

Zebrafish—on the move towards ophthalmological research

Chhetri

Jacobson

Gueven

2014

Eye

138

114

View full text Add to dashboard Cite

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“…The side walls of the racetracks were opaque, thus the fish could only view the laptop screen. Larvae were tested specifically at this age because no schooling behavior is observed at this stage of development, 29 yet their vision is already well developed. Larvae were repeatedly exposed to gratings moving in two different directions; six runs of 90-sec duration for six times, three in each direction.…”

Section: Visual Testing Proceduresmentioning

confidence: 99%

Gucy2f zebrafish knockdown – a model for Gucy2d-related leber congenital amaurosis

et al. 2012

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Mutations in retinal-specific guanylate cyclase (Gucy2d) are associated with Leber congenital amaurosis-1 (LCA1). Zebrafish offer unique advantages relative to rodents, including their excellent color vision, precocious retinal development, robust visual testing strategies, low cost, relatively easy transgenesis and shortened experimental times. In this study we will demonstrate the feasibility of using gene-targeting in the zebrafish as a model for the photoreceptor-specific GUCY2D-related LCA1, by reporting the visual phenotype and retinal histology resulting from Gucy2f knockdown. Gucy2f zebrafish LCA-orthologous cDNA was identified and isolated by PCR amplification. Its expression pattern was determined by whole-mount in-situ hybridization and its function was studied by gene knockdown using two different morpholino-modified oligos (MO), one that blocks translation of Gucy2f and one that blocks splicing of Gucy2f. Visual function was assessed with an optomotor assay on 6-days-postfertilization larvae, and by analyzing changes in retinal histology. Gucy2f knockdown resulted in significantly lower vision as measured by the optomotor response compared with uninjected and control MO-injected zebrafish larvae. Histological changes in the Gucy2f-knockdown larvae included loss and shortening of cone and rod outer segments. A zebrafish model of Gucy2f-related LCA1 displays early visual dysfunction and photoreceptor layer dystrophy. This study serves as proof of concept for the use of zebrafish as a simple, inexpensive model with excellent vision on which further study of LCA-related genes is possible. Keywords: leber congenital amaurosis; animal model; GUCY2D; optomotor assay INTRODUCTION Leber congenital amaurosis (LCA) is a family of severe, early-onset, autosomal-recessive forms of pediatric blindness. LCA has been linked to more than 16 genes, often exhibiting clinical heterogeneity. 1,2 LCA1 is caused by mutations in the gene GUCY2D, which encodes the retinal Gucy2d-1 (GC1) and accounts for B20% of all cases of LCA. 3 LCA2 is caused by mutations in the RPE65 gene, which is expressed in the retinal pigment epithelium. Breakthrough research has demonstrated visual improvement following gene therapy trials in humans with LCA2. 4,5 Human trials relied on a decade of proof-of-concept studies in canine and rodent models. 6-8 Unlike LCA2, in which gene therapy is aimed at correcting an RPE defect, searching for gene therapy for LCA1 will involve an attempt at treating the photoreceptors. European Journal of Human GeneticsResearch in inherited retinal disease relies heavily on animal models, commonly mammals such as mice and dogs. These animal models have significant advantages, including the availability of knockout technology. Two specific drawbacks slow the applicability of rodent or canine models for study of relatively rare genetic diseases such as LCA and other retinal dystrophies. The first difficulty is the high cost and length of time required to create a new knockout line; thus, it is applicable only for comm...

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Assessment of Effects on Visual Function in Larval Zebrafish

Alderton¹

2011

Zebrafish

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Visual Behavior in Zebrafish

Cited by 170 publications

References 66 publications

Zebrafish—on the move towards ophthalmological research

Zebrafish—on the move towards ophthalmological research

Gucy2f zebrafish knockdown – a model for Gucy2d-related leber congenital amaurosis

Assessment of Effects on Visual Function in Larval Zebrafish

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