Visual Biosensing of Bovine Serum Albumin Using Induced Aggregation of Gold Nanoparticles via 3-Aminopropyl Triethoxysilane

Farrell, Monique; Rutherford, Gugu; Pradhan, A. K.

doi:10.1166/jnn.2016.12398

Cited by 3 publications

(3 citation statements)

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“…This causes a systemic blue shift on the application of heat to the protein. Similar phenomenon was observed for some other proteins, such as polynucleotides [18], bovine serum albumin [23], and glutathione peptides [39] with a reproduceable results. The current results are significantly important compared to other reports as we present the size and size distribution of denatured vs. non-denatured ferritin protein, which is a real constituent of our blood.…”

Section: Resultssupporting

confidence: 83%

“…We have developed a unified method for the visual detection of denatured forms of proteins utilizing concentration-dependent aggregation of AuNps via APTES. From previously published work, it is known that the addition of an aminosilane to gold nanoparticles will induce extensive shifts in the absorbance spectra and can be exploited to visually detect stable proteins [22,23]. With the target goal of ferritin visual detection, a highly sensitivity experiment was employed to confirm viability of ferritin for the proposed study.…”

Section: Resultsmentioning

confidence: 99%

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Optical Detection of Denatured Ferritin Protein via Plasmonic Gold Nanoparticles Exposure through Aminosilane Solution

et al. 2019

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The presence of denatured proteins within a therapeutic drug product can create a series of serious adverse effects, such as mild irritation, immunogenicity, anaphylaxis, or instant death to a patient. The detection of protein degradation is complicated and expensive due to current methods associated with expensive instrumentation, reagents, and processing time. We have demonstrated here a platform for visual biosensing of denatured proteins that is fast, low cost, sensitive, and user friendly by exploiting the plasmonic properties of noble metal nanoparticles. In this study we have exposed artificially heat stressed ferritin and gold nanoparticles to 3-aminopropyl triethoxysilane, which degrades the protein by showing a systematic blue shift in the absorbance spectra of the gold nanoparticle/ferritin and aminosilane solution. This blue shift in absorbance produces a detectable visual color transition from a blue color to a purple hue. By studying the Raman spectroscopy of the gold nanoparticle/ferritin and aminosilane solution, the extent of ferritin degradation was quantified. The degradation of ferritin was again confirmed using dynamic light scattering and was attributed to the aggregation of the ferritin due to accelerated heat stress. We have successfully demonstrated a proof of concept for visually detecting ferritin from horse spleen that has experienced various levels of degradation, including due to heat stress.

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Section: Resultssupporting

confidence: 83%

Section: Resultsmentioning

confidence: 99%

Optical Detection of Denatured Ferritin Protein via Plasmonic Gold Nanoparticles Exposure through Aminosilane Solution

et al. 2019

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“…[19][20][21] From previous work, we understood that bovine serum albumin (BSA) could be detected visually using gold nanoparticles in the presence of 3-Aminopropyltreithoxysilane (APTES). 22 The presence of the APTES within the gold nanoparticle solution induces a dipole causing aggregation and subsequent visual changes in the solution from red to purple to blue depending on the concentrations of APTES. The addition of a protein to the solution before the addition of APTES dictates the extent of aggregation upon exposure of the protein/gold nanoparticles solution to APTES.…”

mentioning

confidence: 99%

Visual in Solution Detection of Denatured Insulin Coupled to Gold Nanoparticles in the Presence of an Aminosilane

Farrell

Hawley

Reaume

et al. 2016

J. Electrochem. Soc.

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The pharmaceutical industry is comprised of a myriad of active pharmaceutical ingredients, all applied to increasing human health and quality of life. One of the requirements of reaping the health benefits of these drug products is maintaining them in a native or desired form. Such non-native forms can include, aggregated or fragmented structures. Unfortunately, the most widely used methods to detect non-native or denatured proteins require trained technicians, bulky instrumentation and large amounts of reagents. Deviation from the native structures can occur at all stages; from manufacturing and processing to storage. With these limitations in mind, a simplistic and highly sensitive in solution detection method was evaluated to visually detect denatured insulin proteins, utilizing gold nanoparticle aggregation via 3-Aminopropyltreithoxysilane. The insulin in this study was heat stressed using an 80 • C water bath to create an accelerated heat stressed environment. The insulin, gold nanoparticle and aminosilane solution was then characterized utilizing, UV-Vis spectroscopy, dynamic light scattering and scanning electron microscopy. Captured images and resulting absorbance spectra of the trials demonstrated visual color changes detectable with the human eye as a function of the denaturation time. This work serves as an extended proof of concept for fast in solution detection methods for proteins that have experienced heat stress.

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Visual Detection of Biomolecules Using Concentration Dependent Induced Aggregation of Plasmonic Gold Nanoparticles

Farrell

Pradhan

2022

Micro

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Significant advancement has occurred in the detection methods of solution-based analytes. High-pressure liquid chromatography, gas chromatography, and other systems used for analyses are quite expensive. Therefore, there is a need for new methods and for the visible detection of analytes. Here, we demonstrate that 3-aminopropyl triethoxysilane (APTES) could impact the stability, optical, and morphology of gold nanoparticles (AuNps) in a colloidal solution. These impacts can be used to create a sensitive visual detection system. The strong impact of the APTES concentration on the ultraviolet–visible absorption spectra of the solutions is illustrated, which displays systematic and extensive red shifts. The presence of denatured proteins within a therapeutic drug product can induce a series of adverse effects. This report describes a fast, low cost, sensitive, and user-friendly platform where the plasmonic nanoparticles create visual biosensing of denatured proteins. Artificially heat stressed ferritin, glutathione, and insulin coupled to AuNps are exposed to ATES and upon denaturation of the protein or peptide, systematic blue or red shifts are observed in the absorbance spectra of the AuNps/biomolecules, and aminosilane solution. This serves as a proof-of-concept for a fast in-solution detection method for heat-stressed proteins or peptides.

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Visual Biosensing of Bovine Serum Albumin Using Induced Aggregation of Gold Nanoparticles via 3-Aminopropyl Triethoxysilane

Cited by 3 publications

References 0 publications

Optical Detection of Denatured Ferritin Protein via Plasmonic Gold Nanoparticles Exposure through Aminosilane Solution

Optical Detection of Denatured Ferritin Protein via Plasmonic Gold Nanoparticles Exposure through Aminosilane Solution

Visual in Solution Detection of Denatured Insulin Coupled to Gold Nanoparticles in the Presence of an Aminosilane

Visual Detection of Biomolecules Using Concentration Dependent Induced Aggregation of Plasmonic Gold Nanoparticles

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