Visual Color-Switch Electrochemiluminescence Biosensing of Cancer Cell Based on Multichannel Bipolar Electrode Chip

Abstract

In this work, we developed a visual electrochemiluminescence (ECL) sensing platform based on a dual-bipolar electrode (D-BPE) array chip. The chip was composed of two arrays of BPEs and three separated arrays of reservoirs filled with buffer, Ru(bpy)3(2+)-TPrA and luminol solutions, respectively. Both BPEs served as ECL reporting platforms. By applying 6.0 V voltage, an array of orange electrochemiluminescence (ECL) signals belonging to Ru(bpy)3(2+) turned on. After adding DNAzyme and H2O2 in Ru(bpy)3(2+) and luminol reservoirs, the orange Ru(bpy)3(2+) signals decreased until vanished due to the quenching effect; meanwhile, a new array of blue ECL signals turned on because of the luminol-H2O2 ECL reaction. The designed D-BPE owns superior properties compared with the three-electrode system benefiting from the quantitative relation of bipolar systems, which greatly enhanced the ECL detection sensitivity. Meanwhile, the visual color-switch ECL and the ratiometric detecting principle made the results easier to evaluate and more accurate. Quantitative detection of HL-60 cancer cells from 320 cells/mL to 2.5 × 10(5) cells/mL with two linear ranges was achieved. The detection limit was down to 80 cells/mL. Finally, this D-BPE chip could distinguish the tumor cells from normal cells and provide a prospect for cancer diagnosis in a high-throughput, visual way.

Dual-electrochromic bipolar electrode-based universal platform for the construction of various visual advanced logic devices

“…ABTS can be effectively oxidized by H 2 O 2 and turns green in the typical colorimetric reaction. 39 In addition, as H 2 O 2 has a low redox potential, 40 the supplied driving voltage can also effectively promote the oxidation of ABTS in Rb and the deposition of PB at the Figure S4.…”

Capillary‐assisted bipolar electrochemistry: A focused mini review

“…Such a platform coupled to an amplification strategy allows to detect very small amounts of specific cell surface proteins . The detection of cancer biomarkers thanks to a BPE array chip was also demonstrated, thus opening the door to sensitive and reasonably cheap high‐throughput analytical detection on biochips .…”

Design and Application of Multi-functional Electrogenerated Chemiluminescence Imaging Analyzer

“…An electrochemical cell (ECL cell, 20 × 12 mm, 2 mm thick, 600 μL) was designed by using ethylene-vinyl acetate copolymer as the insulating dielectric material. A 2 × 3 bipolar gold electrode was fabricated according to the reference 25 and the preparation process of the bipolar electrode system in this study is shown in Fig. S2 (Supporting Information).…”